Activity of antioxidant enzymes in seminal plasma and their relationship with lipid peroxidation of spermatozoa

Abstract

Purpose: To determine the activity of seminal plasma catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GPX) and their relationship with malondialdehyde (MDA), as a marker of lipid peroxidation, content of spermatozoa and seminal plasma in normozoospermic and asthenozoospermic males. Materials and Methods: Semen samples were obtained from 15 normozoospermic and 30 asthenozoospermic men. Results: We observed inverse correlations between activities of CAT (k/mL) and SOD (U/mL) in seminal plasma with MDA content of spermatozoa from normozoospermic samples (r =-0.43, p < 0.05 and r =-0.5, p < 0.05, respectively). Significant correlations were observed between total activity CAT (k/total seminal plasma) with total SOD (U/total seminal plasma) and GPX activity (mU/total seminal plasma) in seminal plasma from normozoospermic samples (r = 0.67, p = 0.008 and r = 0.455, p = 0.047, respectively). Furthermore, we found positive correlations between total activities of CAT, SOD and GPX with total content of MDA in seminal plasma (nmoL/total seminal plasma) from normozoospermic samples (r = 0.67, p = 0.003; r = 0.73, p = 0.003; r = 0.74, p = 0.004, respectively). In asthenozoospermic samples, there were no significant correlations observed between activities of CAT (k/mL), SOD (U/mL) and GPX (mU/mL) of seminal plasma with MDA content of spermatozoa. However, we found significant correlations between total activities of CAT (k/total seminal plasma) and SOD (U/total seminal plasma) with total content of MDA in seminal plasma (r = 0.4, p = 0.018 and r = 0.34, p = 0.03, respectively). Conclusion: These findings indicate a protective role for antioxidant enzymes of seminal plasma against lipid peroxidation of spermatozoa in normozoospermic samples.

Zinc and Iron Concentration and SOD Activity in Human Semen and Seminal Plasma

“…These findings are in accordance with the study of Murawski et al [34]. It must be emphasized that SOD seminal plasma provides protection against lipid peroxidation of phospholipid and phospholipid-bound fatty acids in normozoospermic samples [35,36]. The results of other studies, however, indicate that SOD activity in seminal plasma is lower in men with normozoospermia than in men with pathological spermiogram [37,38].…”

Glutathione peroxidase activity in seminal plasma and its relationship to classical sperm parameters and in vitro fertilization-intracytoplasmic sperm injection outcome

“…Although a number of studies have investigated the relationship between human seminal plasma GPX and semen parameters, results have not been consistent. Lower GPX levels have been reported among infertile men (27,28), as well as in patients with asthenozoospermia (29), while in other studies no relationship between these parameters was detected (30)(31)(32), and, surprisingly, in another study, higher levels of GPX were reported in males with oligozoospermia or asthenozoospermia (33). These discrepancies may be explained by sample sizes.…”

Efecto del isoespintanol y el timol en la actividad antioxidante de semen equino diluido con fines de congelación

“…La actividad de la GPx y la superóxido dismutasa (SOD) provenientes de la fracción conservada de plasma seminal se midieron mediante los kits Ransel ® y Ransod ® (Randox Laboratories, Crumlin, UK), respectivamente, de acuerdo con los procedimientos descritos por Tavilani y colaboradores (25). Para la GPx se preparó una solución de reacción con glutatión (4 mmol/L), NADPH (0,34 mmol/L) y glutatión reductasa (0,5 U/L).…”