Activity of ATPases in postmitichondtial fraction of lymphoma NK/Ly cells under bafilomicine and NAADP presence

Bychkova, S.; Hreniuh, V. P.

doi:10.30970/sbi.0902.413

Cited by 4 publications

(12 citation statements)

References 4 publications

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“…These data suggested that bafilomycin A1 may inhibit complex I of respiratory chain or α‐ketoglutarate dehydrogenase leading to decrease in mitochondrial oxygen consumption. Effects of bafilomycin A1 on respiration of mitochondria may be related to the fact that mitochondria fraction contains some portion of autophagosomes . We hypothesized that bafilomycin A1 targeting H + ‐ATPase can acidify extramitochondrial volume and decrease the Ca 2 + uptake by mitochondria.…”

Section: Resultsmentioning

confidence: 99%

“…Mitochondria present a unique set of key intracellular functions such as ATP synthesis, production of ROS, and Ca 2 + buffering. It has to be pointed that “unwashed” mitochondrial suspension used in this work contains several other subcellular organelles such as autophagosome and other . It is not surprising because autophagy has important role in selective elimination of damaged mitochondria.…”

Section: Resultsmentioning

confidence: 99%

“…Effects of bafilomycin A1 on respiration of mitochondria may be related to the fact that mitochondria fraction contains some portion of autophagosomes. 40 We hypothesized that bafilomycin A1 targeting H + -ATPase can acidify extramitochondrial volume and decrease the Ca 2+ uptake by mito- Table 1. ADP/O did not differ in control group with both α-ketoglutarate and succinate.…”

Section: Effects Of Naadp and Bafilomycin A1 On Parameters Of Respimentioning

confidence: 99%

“…It has to be pointed that "unwashed" mitochondrial suspension used in this work contains several other subcellular organelles such as autophagosome and other. 40 It is not surprising because autophagy has important role in selective elimination of damaged mitochondria.…”

Section: Effects Of Naadp and Bafilomycin A1 On Parameters Of Respimentioning

confidence: 99%

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Effect of bafilomycin and NAADP on membrane‐associated ATPases and respiration of isolated mitochondria of the murine Nemeth‐Kellner lymphoma

Hreniukh

Bychkova

Kulachkovsky

et al. 2016

Cell Biochemistry & Function

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Bafilomycin A1 potentiates the effect of NAADP by inhibiting the mitochondrial energetic process in lymphoma cells and activity of Na /K -ATPase. The obtained data show promising possibility to use bafilomycin A1 and NAADP as chemotherapeutic agents for lymphoma cells treatment. This is important because lymphomas are seventh most common form of cancer. Today the lymphoma mortality is 15% to 30%, whereas the effectiveness of malignant neoplasms treatment is less than 50%.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Effects Of Naadp and Bafilomycin A1 On Parameters Of Respimentioning

confidence: 99%

Section: Effects Of Naadp and Bafilomycin A1 On Parameters Of Respimentioning

confidence: 99%

See 2 more Smart Citations

Effect of bafilomycin and NAADP on membrane‐associated ATPases and respiration of isolated mitochondria of the murine Nemeth‐Kellner lymphoma

Hreniukh

Bychkova

Kulachkovsky

et al. 2016

Cell Biochemistry & Function

Self Cite

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“…Assay of ATPase activity. ATPase activity was determined according to the content of orthophosphate that was released after ATP hydrolysis [8,9]. At the beginning of the experiment 200 μlof post-mitochondrial subcellular fraction of patients' colon mucous was transferred to a standard incubation medium containing (mM) NaCI -50.0; KCI -100.0; Tris-НСІ -20.0; MgCI 2 -3.0; CaCI 2 -0.01; рН 7.4 at 37 °С.…”

Section: Methodsmentioning

confidence: 99%

Peculiarities of the Effects of Bile Acids on Atpase Activity of the Colon Mucosa in Patients With Overweight and Irritable Bowel Syndrome

2020

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The aim is to investigate the effect of bile acids on the ATPase activity of the colon mucosa in patients with overweight and irritable bowel syndrome (IBS). Materials and methods: Completely examined 12 patients with IBS and overweight.We estimated the ATPase activity of colon mucous of the patients with IBSspectrophotometrically by determined the content of orthophosphate that was released after ATP hydrolysis. We studied the effect of 3-sulphate of taurolitocholate (TLC-S) on specific activities of Na+/ K+-ATPase, Ca2+-ATPase of endoplasmatic reticulum (EPR),Ca2+-ATPase of plasmatic membrane (PM) and basal Mg2+-ATPase of postmitochondrial subcellular fraction of colon mucous of the patients with IBS. Results: We establishedthe specific activities of Na+/K+-ATPase, Ca2+-ATPase of EPR,Ca2+-ATPase of PM and basal Mg2+-ATPase. Therewere(6.06 ± 1.61), (5.88 ± 1.19), (8.86 ± 1.56) (6.44 ± 2.02)μmol Pi/ mg protein per hour, respectively. TLC-S (50 μM) did not causedany change of Na+/K+-ATPase , as well as Ca2+-ATPasesactivities, but statistically significant increased activity of Mg2+-ATPase of postmitochondrial subcellular fraction of colon mucous of the patients with IBS by 4 fold. Conclusions: TLC-S increased basal Mg2+-ATPase in the postmitochondrial fraction of colon mucous of the patients with overweight and IBS, but had no effect on Na+/K+-ATPase and Ca2+-ATPases activities. It has been suggested that activation of basal Mg2+-ATPase under by TLC-S may indicates the role of the endo-lysosomal system of epitheliocytes of colon mucous in developing of pathology IBS.

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The role of bafilomycin as a therapeutic agent in the modulation of endo-lysosomal store of rat hepatocytes

2022

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Endo-lysosomal system through the process of autophagy is involved in the pathogenesis of many diseases. Acidification of these organelles is carried out by V-type H+-ATPases, which is inhibited by bafilomycin A1. Endosomes and lysosomes are also important Ca2+-storage in a cell. Nіcotіnіc acіd adenіne dіnucleotіde phosphate (NAADP) releases Cа2+ from endo-lysosomes. The main purpose of the study was to found out the effect of bafilomycin A1 and NAADP on stored Ca2+ and on the ATPase activity of rat hepatocytes. The stored Ca2+ was estimated using chlorotetracycline in permeabilized hepatocytes of rats. ATPase activity was determined by level of orthophosphate spectrophotometrically. It was found that bafilomycin A1 reduces stored Ca2+ in permeabilized hepatocytes of rats in the micromolar range of concentration (20 and 0.04 mkM) and averted the effect of NAADP on calcium content. Lower concentrations of bafilomycin A1 (0.001 mkM) did not alter the content of stored calcium, but prevented the influence of NAADP in permeabilized hepatocytes of rats. In the subcellular fraction of rat liver bafilomycin A1 (0.001 mkM) increased Ca2+-ATPase and basal Mg2+-ATPase activities and reduced Na+/K+-ATPase activity. Preincubation of the subcellular fraction with bafilomycin A1 completely averts any changes in the activity of estimated ATPases by means of NAADP. It was concluded that the bafilomycin-sensitive store in hepatocytes of rats is NAADP-sensitive endo-lysosomal Ca2+-store. Using of bafilomycin A1 may be useful in treating autophagy-depended diseases.

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Activity of ATPases in postmitichondtial fraction of lymphoma NK/Ly cells under bafilomicine and NAADP presence

Cited by 4 publications

References 4 publications

Effect of bafilomycin and NAADP on membrane‐associated ATPases and respiration of isolated mitochondria of the murine Nemeth‐Kellner lymphoma

Effect of bafilomycin and NAADP on membrane‐associated ATPases and respiration of isolated mitochondria of the murine Nemeth‐Kellner lymphoma

Peculiarities of the Effects of Bile Acids on Atpase Activity of the Colon Mucosa in Patients With Overweight and Irritable Bowel Syndrome

The role of bafilomycin as a therapeutic agent in the modulation of endo-lysosomal store of rat hepatocytes

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