Activity of catalase enzyme in Paulownia tomentosa seeds during the process of germination after treatments with low pressure plasma and plasma activated water

Abstract

In this work we present results of two significantly different types of plasma treatment on Paulownia tomentosa Steud. seeds. In the first type, seeds were directly treated in low‐pressure plasma and then imbibed with distilled water. In the second type, an atmospheric pressure plasma was used for obtaining plasma activated water (PAW) which is then used for imbibition of seeds. The CAT activity and protein content is evaluated during 4 d following the imbibition process, i.e., immediately after the phytochrome activation and in the 3 subsequent days. Comparison of results of treated seeds to the control group allows to correlate the enzyme activity and protein content during the initial stages of germination with plasma treatment types and treatment conditions.

Effects of plasma activated water on wheat: Germination, growth parameters, photosynthetic pigments, soluble protein content, and antioxidant enzymes activity

“…On the contrary, seeds germinated in the PAW or plants irrigated with the PAW, as it is in our experiments, showed decrease of antioxidant enzymes activity. However, the activity can also be affected by others parameters, for example, growth phase of plants . Unfortunately, there are no studies dealing with the monitoring of antioxidant enzymes activity in plants irrigated with the PAW (related to indirect plasma treatment) to make more specific conclusion.…”

“…Several papers reported antioxidant enzymes activity and concluded that plasma in direct contact with plant cell increases the activity . On the contrary, seeds germinated in the PAW or plants irrigated with the PAW, as it is in our experiments, showed decrease of antioxidant enzymes activity. However, the activity can also be affected by others parameters, for example, growth phase of plants .…”

“…The indirect plasma treatment/activation of seeds and plants by the CP, that is, the effect of plasma activated gas or plasma activated water (PAW) is far less studied than the direct plasma effect even though it can also enhance seed germination and plant growth. The PAW can be generated by operating electric discharges directly in water but more often in gas in a contact with water, usually above the water surface, for example, gliding arc, glow discharge, dielectric barrier discharge, or plasma jets . Besides pure water also other liquid media, such as drainage water from pots, nutrient solution, or fertilizer have been activated and used.…”

Plasma‐activated water to improve the stress tolerance of barley

“…In the last few years, studies have been conducted on selected species to test the effect of PAW on seed germination and plant growth …”

From patent to product? 50 years of low‐pressure plasma sterilization

“…While additional techniques can be considered to observe protein folding (circular dichroism spectroscopy reference) or the introduction of backbone breaks (e.g., gel electrophoresis, besides other techniques), such experiments require additional facilities and expertise. To circumvent this, some studies use enzymes which have easy‐to‐monitor functions as models such as GapDH, RNaseA, or catalase …”