Activity of Debaryomyces hansenii UFV-1 α-galactosidases against α-d-galactopyranoside derivatives

Viana, Pollyanna Amaral; Rezende, Sebastião Tavares de; Alves, Arianne de A.; Manfrini, Rozângela Magalhães; Alves, Ricardo José; Bemquerer, Marcelo P.; Santoro, Marcelo M.; Guimarães, Valéria Monteze

doi:10.1016/j.carres.2011.01.024

Cited by 9 publications

(7 citation statements)

References 20 publications

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“…Debaryomyces hansenii is prevalent in seawater, and sequences affiliated with D. hansenii have been reported in hydrothermal sediments (Bass et al ., ) which may explain its high incidence in fish. A major biotechnological advantage of D. hansenii is its osmotolerance and its huge enzymatic potential (Breuer and Harms, ), reflected by the synthesis of inulinase (Gao et al ., ), protease (Bolumar et al ., ), superoxide dismutase (Garcia‐Gonzalez et al ., ), lipase (Takac and Sengel, ), catalase (Segal‐Kischinevzky et al ., ) and α‐galactosidase (Viana et al ., 2006; 2007; 2009; 2011a,b). The α‐galactosidase from D. hansenii has been useful in the in vitro treatment of soybean products to reduce raffinose oligosaccharides (Viana et al ., ), which are recognized anti‐nutritional factors (ANF) for mammals and fish.…”

Section: Discussionmentioning

confidence: 99%

Debaryomyces hansenii and Rhodotorula mucilaginosa comprised the yeast core gut microbiota of wild and reared carnivorous salmonids, croaker and yellowtail

Raggi

López

Díaz

et al. 2014

Environmental Microbiology

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This is the first study using molecular and culture-based methods aimed at investigating the composition of the intestinal yeast microbiota of wild and reared carnivorous salmonids, croaker and yellowtail, to characterize their cores and to evaluate the enzymatic activities of the cultivated yeast. Among 103 samples from salmonids, croaker and yellowtail, yeast were detected in 85.4%, with 43 species identified. The core of reared fish was composed of eight species, in contrast to the wild fish core, which consisted of two species: Debaryomyces hansenii and Rhodotorula mucilaginosa. Despite the smaller diversity of the wild fish core, similar enzymatic profiles were detected for the species from the wild and reared cores. For principal component analysis, samples grouped together independently of host species, domestication status and location. A high proportion of yeast produced aminopeptidases and lipases, which may be explained by the high proportion of protein and lipids in the carnivorous diet. This study reveals the presence of a yeast community in the fish gut that appears to be strongly shaped by a carnivorous diet. Yeast in the gut increases the repertoire of microorganisms interacting with the host intestine, which could influence health and disease.

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Section: Discussionmentioning

confidence: 99%

Debaryomyces hansenii and Rhodotorula mucilaginosa comprised the yeast core gut microbiota of wild and reared carnivorous salmonids, croaker and yellowtail

Raggi

López

Díaz

et al. 2014

Environmental Microbiology

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“…D. hansenii has been extensively studied because of its significant enzymatic potential [52]. For example, several enzymes of biological and biotechnological interest have been identified and characterized in this yeast, including inulinase [53], protease [54], superoxide dismutase (SOD) [55], lipase [56], catalase [57] and α-galactosidase [58][59][60][61][62]. Additionally, β-glucosidase from D. pseudopolymorphus [63] and phytase from D. castellii [64] has also been identified.…”

Section: Debaryomyces Hansenii An Ubiquitous Yeast Frequently Associmentioning

confidence: 99%

Use of Yeasts as Probiotics in Fish Aquaculture

Navarrete¹,

Tovar‐Ramírez²

2014

Sustainable Aquaculture Techniques

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Yeasts are unicellular eukaryotic microorganisms that are taxonomically placed within the phyla Ascomycota and Basidiomycota within the Kingdom Fungi [7]. Ascomycete yeasts comprise approximately 1,000 phylogenetically diverse species that have recently been assigned to 14 different lineages on the basis of multigene sequence analysis [8]. The other species of yeasts are classified as the basidiomycetes [9]. Some of the general characteristics and ecological properties of each phylum include the following: 1) the cell wall polysaccharide composition is dominated by chitin in the basidiomycetes and by β-glucans in the ascomycetes; 2) the guanine + cytosine (G + C) composition of the nuclear DNA tends to be higher than 50% in basidiomycetes and lower than 50% in ascomycetes; 3) ascomycetes yeasts are generally more fermentative, more copiotrophic (but at the same time nutritionally specialized), more fragrant, and mostly hyaline, while basidiomycete yeasts more often form mucoid colonies, Sustainable Aquaculture Techniques 136 Marine samples Identified yeast Identification method Reference

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“…More importantly, our data suggest that broader changes in community structure may be important to consider when investigating the metabolic consequences of the gut microbiota. Methyl-galactoside itself is an intriguing metabolite as it has anti-fungal activity towards certain fungi 42 . Since gut bacteria and fungi community live together and influence each other 43 , it is highly likely that the alteration of certain fungal abundance can influence a number of gut bacteria and vice versa.…”

Section: Discussionmentioning

confidence: 99%

Microbial modulation of host body composition and plasma metabolic profile

Huda

Winnike

Crowell

et al. 2020

Sci Rep

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The gut microbiota is a critical mediator of nutrition and disease risk. Like most complex traits, the microbiome is under genetic regulation and differs between inbred strains of mice. We tested the effect of fecal microbiota transplantation (FMT) on obesity, and plasma glucose. For this study, we collected microbiota from 2 inbred strains of mice which differ in adiposity and glucose tolerance, C57BL/6J and WSB/EiJ. C57BL/6J female mice (n = 18) were first treated with antibiotics for 4 weeks to ablate the microbiota. Following ablation, the mice were transplanted with microbiota from a C57BL/6J or a WSB/ EiJ mouse and clinical traits and plasma metabolomic profiles were interrogated at 2-and 4-weeks post-transplantation. Unexpectedly, the mice receiving WSB/EiJ microbiota increased adiposity but decreased plasma glucose. Metabolomic and 16S microbiota profiling indicated broad metabolic changes occurred during and after FMT. Detailed analysis of these interactions demonstrated specific microbiota-host metabolite interactions which may alter disease susceptibility.Bacteria is heavily colonized in the gastrointestinal tract, often referred to as the gut microbiota (hereafter referred to as microbiota), which can modulate nutritional status, health, and diseases of the host 1 . The diversity and composition of the gut bacteria have been intensely studied, as well as the microbiota's impact on the health status of the host 2 . The microbiota has been found to be associated with susceptibility to multiple diseases including: obesity 3 , cardiovascular diseases 4 , renal diseases 5 , and metabolic syndrome (MetSyn) 6 , all of which have impact on major public health. For example, 32% of American adults are obese 7 and nearly 35% have MetSyn 8 and the prevalence of these diseases are increasing. However, there are still critical gaps in our knowledge regarding how alterations of the microbiota (dysbiosis) can alter metabolism and thus disease susceptibility. A better understanding of the effect of host genetic-microbiota interactions on the composition of the microbiota and disease susceptibility is needed. A holistic view of the metabolic status of an individual, including both microbiota and host genetics, may provide new insights into the underlying mechanisms of pathobiology which may allow us to modulate disease onset, prognosis, and survival 9,10 .Metabolomic profiling may provide additional insight into the processes affected by specific bacteria or when the composition of the microbiota is altered 11 . It has been shown that the gut microbiota can have a significant effect on plasma metabolic profile, which can modulate host health 12 . Therefore, pairing metabolomics and microbiota analysis may yield important mechanistic insights. For example, trimethylamine N-oxide (TMAO) was identified as a risk factor for cardiovascular disease through a metaorganismal pathway involving the microbiota and diet 13 .Laboratory mice are often used to investigate both genetic and microbiota to understand the underlying mecha...

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Activity of Debaryomyces hansenii UFV-1 α-galactosidases against α-d-galactopyranoside derivatives

Cited by 9 publications

References 20 publications

Debaryomyces hansenii and Rhodotorula mucilaginosa comprised the yeast core gut microbiota of wild and reared carnivorous salmonids, croaker and yellowtail

Debaryomyces hansenii and Rhodotorula mucilaginosa comprised the yeast core gut microbiota of wild and reared carnivorous salmonids, croaker and yellowtail

Use of Yeasts as Probiotics in Fish Aquaculture

Microbial modulation of host body composition and plasma metabolic profile

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