Activity of diastase α-amylase immobilized on polyanilines (PANIs)

Ashly, P.C.; Joseph, Mini; Mohanan, Pezholil

doi:10.1016/j.foodchem.2011.02.068

Cited by 56 publications

(25 citation statements)

References 31 publications

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“…The immobilization procedure shifted the optimum pH value for the α ‐amylase to a higher pH value, which indicates that the distribution of the hydrogen and hydroxyl ions were different in the area close to the surface from the remainder of the solution . The similar observation was reported by Ashly et al and Nwagu et al for covalently immobilized α ‐amylase. The pH profile of the immobilized α ‐amylase was broader than that of the free α ‐amylase.…”

Section: Resultssupporting

confidence: 82%

Immobilization of α‐amylase on reactive modified fiber and its application for continuous starch hydrolysis in a packed bed bioreactor

Temoçin

2013

Starch Stärke

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In this study, the enzyme α‐amylase was immobilized on reactive modified poly (ethylene terephthalate) fiber. The activities of free α‐amylase and immobilized α‐amylase were compared in a batch starch hydrolysis system. Optimum pH and Michaelis–Menten constants were determined for both free α‐amylase and immobilized α‐amylase. The immobilization shifted the optimum pH to a higher level. The Michaelis–Menten constant values (Km) of the immobilized α‐amylase and free α‐amylase were obtained as 11.94 and 6.61 mg/mL, respectively. The experiments indicated that the immobilization increased the thermal stability of the α‐amylase. The immobilized α‐amylase, easily separated from the reaction media, could be used effectively for 15 cycles with 65% retention of the initial activity. Moreover, the immobilized α‐amylase was effectively used in packed bed bioreactor for continuous starch hydrolysis throughout 6.5 h without loss of activity. Different starches (e.g., potato and wheat) were hydrolyzed by the immobilized α‐amylase in continuous process. The results indicate a potential use of this immobilized enzyme system for the construction of packed bed bioreactors to be used in the hydrolysis of the starch.

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Section: Resultssupporting

confidence: 82%

Immobilization of α‐amylase on reactive modified fiber and its application for continuous starch hydrolysis in a packed bed bioreactor

Temoçin

2013

Starch Stärke

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“…It was depicted in the figure 1c. The effect of enzyme concentration on activity also exhibited in the figure 1d and the decrease in the activity after the saturation point is due to conformational changes of the enzyme or the steric hindrance caused by the support at the active site [38]. The immobilization yield, activity yield and immobilization efficiency were calculated and the results are given in the table1.…”

Section: Optimization Of Immobilization Parametersmentioning

confidence: 97%

“…The subsequent decrease in activity after every cycle could be due to desorption, denaturation or conformational changes of the enzyme molecule upon uses. The reusability of immobilized α-amylase was investigated in several reports [47][48][49]. …”

Section: Effect Of Frequent Use On Activity Of Immobilized Enzymesmentioning

confidence: 99%

Enhanced Stability of Α-Amylase via Immobilization onto Chitosan-Tio2 Nanocomposite

Mohanan¹

2017

NSTOA

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“…Some synthetic polymers used as enzyme supports are stated as follows: polyvinyl chloride that prevents enzyme, cyclodextrin glucosyltransferase from thermal inactivation; polyurethane microparticles derived from polyvinyl alcohol and hexamethyl diisocyanate in the ratio of 1:3 with high enzyme loading and efficiency; UV-curable methacrylated/fumaric acid-modified epoxy that is proposed to be useful for industrial applications; polyaniline in two different forms, viz. emeraldine salt and emeraldine base powder used for covalent binding of α- amylase; glutaraldehyde-activated nylon for immobilizing lipase and UV-activated polyethylene glycol having high porosity employed for wastewater treatment (Abdel-Naby 1999; Kahraman et al 2007; Pahujani et al 2008; Romaskevic et al 2010; Xiangli et al 2010; Ashly et al 2011). …”

Section: Materials Used For Fabrication Of Immobilization Supportsmentioning

confidence: 99%

Enzyme immobilization: an overview on techniques and support materials

2012

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The current demands of the world’s biotechnological industries are enhancement in enzyme productivity and development of novel techniques for increasing their shelf life. These requirements are inevitable to facilitate large-scale and economic formulation. Enzyme immobilization provides an excellent base for increasing availability of enzyme to the substrate with greater turnover over a considerable period of time. Several natural and synthetic supports have been assessed for their efficiency for enzyme immobilization. Nowadays, immobilized enzymes are preferred over their free counterpart due to their prolonged availability that curtails redundant downstream and purification processes. Future investigations should endeavor at adopting logistic and sensible entrapment techniques along with innovatively modified supports to improve the state of enzyme immobilization and provide new perspectives to the industrial sector.

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Activity of diastase α-amylase immobilized on polyanilines (PANIs)

Cited by 56 publications

References 31 publications

Immobilization of α‐amylase on reactive modified fiber and its application for continuous starch hydrolysis in a packed bed bioreactor

Immobilization of α‐amylase on reactive modified fiber and its application for continuous starch hydrolysis in a packed bed bioreactor

Enhanced Stability of Α-Amylase via Immobilization onto Chitosan-Tio2 Nanocomposite

Enzyme immobilization: an overview on techniques and support materials

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