Activity of digestive enzymes along the gut of juvenile red abalone, Haliotis rufescens, fed natural and balanced diets

García-Esquivel, Zaúl; Felbeck, Horst

doi:10.1016/j.aquaculture.2006.08.022

Cited by 60 publications

(47 citation statements)

References 28 publications

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“…3). For abalone species, it has been reported that types and/or levels of digestive enzymes in the gut change in response to chemical compositions of ingested food (e.g., Knauer et al 1996;Johnston et al 2005;Garcia-Esquivel and Felbeck 2006). In the present study, relatively high activities of alginate lyase and CMCase were detected in sea urchins over ≈3 mm in TD (and especially those ≥6 mm in TD) that had consumed the macroalgal diets (Fig.…”

Section: Discussionmentioning

confidence: 49%

Dietary shifts in the juvenile sea urchin Strongylocentrotus intermedius associated with the development of digestive enzymes

et al. 2015

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ingested diets were observed for urchins >3 mm in TD. Our results suggest that the primary nutrient source of S. intermedius shifts from microalgae to macroalgae at ≈6 mm TD, and that macroalgae digestion efficiency in urchins ≥3 mm TD gradually improves because of activation of the enzymes. Highly activated enzymes enable sea urchins to efficiently digest nutrients stored in the bodies of macroalgae, allowing the primary nutrient source of S. intermedius to shift from microalgae to macroalgae.

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Section: Discussionmentioning

confidence: 49%

Dietary shifts in the juvenile sea urchin Strongylocentrotus intermedius associated with the development of digestive enzymes

et al. 2015

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“…The three phosphohydrolases showed uniformly high activities, and there were no consistent rankings among the species or parts of the digestive system. Four carbohydrases (␤-galactosidase, ␤-glucuronidase, N-acetyl-␤-glucosaminidase, and ␣-fucosidase) exhibited very high activities throughout the guts of the abalone and keyhole limpet (Garcia-Esquivel and Felbeck, 2006;Martin et al, 2011) as well as in the calyptraeid hatchlings. ␤-galactosidase and ␤-glucuronidase were also high in the periwinkle, but ␣-fucosidase activity was low compared to the other species.…”

Section: Discussionmentioning

confidence: 99%

“…␤-galactosidase and ␤-glucuronidase were also high in the periwinkle, but ␣-fucosidase activity was low compared to the other species. Since none of these Data from Aplysia punctata (Taïeb, 2001), Haliotis rufescens (Garcia-Esquivel and Felbeck, 2006), Megathura crenulata (Martin et al, 2011), and Littorina irrorata (Bärlocher et al, 1989). Entries in bold are the highest activities in each enzyme category.…”

Section: Discussionmentioning

confidence: 99%

“…However, other herbivorous gastropods have been assayed with the qualitative API system. These include the digestive gland of adult Aplysia sea hares (Taïeb, 2001) and different parts of the digestive system of juvenile red abalone, Haliotis rufescens (Garcia-Esquivel and Felbeck, 2006); the giant keyhole limpet Megathura (Martin et al, 2011); and the periwinkle Littorina (Bär-locher et al, 1989). The values reported for Aplysia do not fit the 0 -5 scale used by the other assays, and they are very different from the other published results (Table 3).…”

Section: Discussionmentioning

confidence: 99%

“…Here we describe a modification of a semi-quantitative color-based enzyme assay (the API-ZYM system) to quantify the activity of 19 enzymes, and use it to survey patterns of enzyme activity during the development of eight species of calyptraeid gastropods. The API-ZYM system shows good agreement with quantitative determinations of enzyme activities in juvenile abalone (Garcia-Esquivel and Felbeck, 2006). Our aims were to determine if the API-ZYM system can be used to detect statistically significant differences in enzyme activities; to document common ontogenetic patterns of enzyme activities; and to identify differences in enzyme expression that may be associated with different modes of development.…”

Section: Introductionmentioning

confidence: 99%

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Comparative Ontogenetic Changes in Enzyme Activity During Embryonic Development of Calyptraeid Gastropods

Collin

Starr

2013

The Biological Bulletin

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Abstract.A modification of a semi-quantitative colorbased enzyme assay was used to quantify the activity of 19 enzymes (5 peptidases, 3 lipases, 3 phosphotases, and 8 carbohydrases) during five stages of development in eight species of calyptraeid gastropods. Sixteen of the 19 enzymes showed a significant effect of mode of development on the concentration of the reaction product after incubation of homogenates standardized for protein content. The overall pattern was that planktotrophs showed the highest activities, followed by adelphophages, and nonfeeding embryos, which had the lowest enzyme activities. Thirteen enzymes showed significant differences across developmental stages. Of these, eight showed a clear increase during development. Only one of the enzymes showed a sudden jump in activity between the unfed, pre-hatching stage and post-hatching stages that were fed Isochrysis galbana. In three cases, ANOVA identified two exclusive, significantly different groups of species. In naphthol-AS-BI-phosphohydrolase, the measured absorbance of Crucibulum spinosum samples was significantly higher than in all of the other species. The activity of ␣-fucosidase in Crepipatella occulta was significantly greater than in the other seven species. Finally, the activity of ␤-galactosidase was significantly higher in C. occulta, Crucibulum spinosum, and Bostrycapulus calyptraeformis than in the four Crepidula species. This is the only enzyme for which there is an indication of a phylogenetic effect. Relative enzyme activities were similar to those reported for other herbivorous gastropods, with the three phosphohydrolases, four carbohydrases (␤-galactosidase, ␤-glucuronidase, N-acetyl-␤-glucosaminidase, and ␣-fucosidase), and leucine arylamidase showing high activities.

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Digestive enzymes of postweaned greenlip abalone (Haliotis laevigata) are influenced by water temperatures and dietary protein levels

Bansemer,

Salini,

Qin

et al. 2023

Aquaculture Fish & Fisheries

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Digestive enzyme activities are known to fluctuate in abalone grown under different environmental conditions however the effects of different dietary regimes are less clear. Therefore, in a 12‐week study, the effects of water temperature and dietary protein levels on digestive enzyme activity in postweaned (6‐month‐old) greenlip abalone (Haliotis laevigata) were investigated. The abalone were fed diets with crude protein levels that ranged from 27% to 36% and cultured at 14°C, 17°C and 20°C. Diets were formulated to be isoenergetic (12.5 MJ kg−1 digestible energy) and isolipidic (3.6% crude lipid). Trypsin, α‐amylase and lipase activities were measured in the gastrointestinal region. Trypsin, α‐amylase and lipase activities significantly increased as water temperatures were raised. In addition, significantly higher α‐amylase activities were observed as protein levels increased, potentially indicating a compensatory effect to increase carbohydrate utilisation for energy metabolism when in short supply. However, abalone fed high dietary protein levels may also utilise protein for energy metabolism. Further research investigating the dietary carbohydrate source for greenlip abalone is recommended. This study provides useful information on the digestive enzyme activity in postweaned greenlip abalone to improve diet strategies for sustainable production.Abbreviated abstract: Post‐weaned greenlip abalone digestive enzyme activity was examined after feeding four dietary protein levels at three different temperatures for a 12‐week period. Enzyme activities were significantly increased with increasing water temperature. Additionally, significantly higher α‐amylase activities were observed as protein levels increased.

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Activity of digestive enzymes along the gut of juvenile red abalone, Haliotis rufescens, fed natural and balanced diets

Cited by 60 publications

References 28 publications

Dietary shifts in the juvenile sea urchin Strongylocentrotus intermedius associated with the development of digestive enzymes

Dietary shifts in the juvenile sea urchin Strongylocentrotus intermedius associated with the development of digestive enzymes

Comparative Ontogenetic Changes in Enzyme Activity During Embryonic Development of Calyptraeid Gastropods

Digestive enzymes of postweaned greenlip abalone (Haliotis laevigata) are influenced by water temperatures and dietary protein levels

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