Activity of enzymes immobilized on plasma treated polyester

Mohamed, Arfaoui; Behary, Nemeshwaree; Mutel, B.; Perwuelz, Anne; Belhacene, Kalim; Dhulster, Pascal; Mamède, Anne-Sophie; Froidevaux, Rénato

doi:10.1016/j.molcatb.2016.09.015

Cited by 14 publications

(26 citation statements)

References 19 publications

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“…Our previous studies explained the formation of hydroxyl and carboxylic groups on the polyester surface aer plasma treatment using the same set-up through various analysis techniques includes; capillary uptake, Fourier transform infrared spectroscopy, energy dispersive spectroscopy and X-ray photoelectron spectroscopy. 37,39,40 All the results clearly correlated to the analysis of sessile water droplet test.…”

Section: Resultssupporting

confidence: 60%

“…12A). 39 As found in the results (discussed in Section 3.2.1) upon oxidation, the dye becomes colourless, indicating the removal of the color bearing group and possible conversion into degraded intermediates. In the initial a Reaction time required for complete colour removal.…”

Section: Part 2: Analysis Of Heterogeneous Fenton-like Removal Of Crymentioning

confidence: 72%

“…37,38 The removal of impurities was assessed by the water break test where surface tension of sample water (from PET rinsing bath) was compared with freshwater (72.6 mN m À1 ). 39 Aer that, twosteps surface modication of polyester membranes was conducted before the immobilization of iron particles. In the rst step, air atmospheric plasma treatment was used to activate the hydrophobic surface of polyester and in a second step, incorporation of PAMAM, APTES, and SH on plasma-treated membranes as explained below;…”

Section: Plasma Treatment and Chemical Graing Of Pamam Aptes And Smentioning

confidence: 99%

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Modification of fibrous membrane for organic and pathogenic contaminants removal: from design to application

et al. 2020

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Section: Resultssupporting

confidence: 60%

Section: Part 2: Analysis Of Heterogeneous Fenton-like Removal Of Crymentioning

confidence: 72%

Section: Plasma Treatment and Chemical Graing Of Pamam Aptes And Smentioning

confidence: 99%

See 1 more Smart Citation

Modification of fibrous membrane for organic and pathogenic contaminants removal: from design to application

et al. 2020

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“…The removal of impurities was assessed by the water break test where the surface tension of sample water (from PN rinsing bath) was measured. The highest cleanliness of PN sample was reached when the surface tension of the rinsing water was that of distilled water (72 mN/m) 25 .…”

Section: Methodsmentioning

confidence: 99%

“…34,35 . The change in nano-roughness (~10 nm) has been shown to have a significant affect on the protein attachment 34 when oxygen 25 as well as nitrogen 36 containing plasma has been used 37 . Therefore, the plasma-discharge parameters should be carefully selected to reach the desired modification that further ensures high surface energy, and therefore improves the biocompatibility without altering substance primary properties.…”

Section: Introductionmentioning

confidence: 99%

Surface modification of polyester fabric using plasma-dendrimer for robust immobilization of glucose oxidase enzyme

Morshed

Behary

Bouazizi

et al. 2019

Sci Rep

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Robust immobilization of glucose oxidase (GOx) enzyme was achieved on poly(ethylene terephthalate) nonwoven fabric (PN) after integration of favourable surface functional groups through plasma treatments [atmospheric pressure-AP or cold remote plasma-CRP (N2 + O2)] and/or chemical grafting of hyperbranched dendrimers [poly-(ethylene glycol)-OH or poly-(amidoamine)]. Absorption, stability, catalytic behavior of immobilized enzymes and reusability of resultant fibrous bio-catalysts were comparatively studied. Full characterization of PN before and after respective modifications was carried out by various analytical, instrumental and arithmetic techniques. Results showed that modified polyester having amine terminal functional groups pledged better surface property providing up to 31% enzyme loading, and 81% active immobilized enzymes. The activity of the enzyme was measured in terms of interaction aptitude of GOx in a given time to produce hydrogen peroxide using colorimetric assay. The immobilized GOx retained 50% of its original activity after being reused six (06) times and exhibited improved stability compared with the free enzyme in relation to temperature. The reaction kinetics, loading efficiency, leaching, and reusability analysis of enzyme allowed drawing a parallel to the type of organic moiety integrated during GOx immobilization. In addition, resultant fibrous bio-catalysts showed substantial antibacterial activity against pathogenic bacteria strains (Staphylococcus epidermidis and Escherichia coli) in the presence of oxygen and glucose. These results are of great importance because they provide proof-of-concept for robust immobilization of enzymes on surface-modified fibrous polyester fabric for potential bio-industrial applications.

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Influence of remote plasma on PEDOT:PSS‐coated carbon felt for improved activity of glucose oxidase

Kahoush

Behary

Cayla

et al. 2019

J of Applied Polymer Sci

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Increasing wettability of carbon felts is an important strategy to improve their efficiency in bio‐electrochemical applications. Herein, influence of cold remote plasma (N2 + O2) treatment on surface properties of carbon felts with poly(3,4‐ethylenedioxythiophene) polystyrene sulfonate (PEDOT:PSS) coating was tested, aiming to improve immobilizing of glucose oxidase enzyme (GOx). Spectra of N 1s and O 1s confirmed the integration of carbonyl and ether as well as amide and amine groups on bare carbon fiber surface, while on coated fibers, carbonyl groups were pre‐dominant. S 2p spectra confirmed oxidation of PEDOT:PSS coating with reduction of (S−) compared to (SO3−) group. GOx immobilized on different samples showed highest activity for PEDOT:PSS coating subjected to plasma with 2% O2, maintaining up to 60% after immobilization, and 37% of its activity after six cycles for some samples. Enzymes immobilized on samples without plasma treatment lost their activity after four cycles. © 2019 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2020, 137, 48521.

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Activity of enzymes immobilized on plasma treated polyester

Cited by 14 publications

References 19 publications

Modification of fibrous membrane for organic and pathogenic contaminants removal: from design to application

Modification of fibrous membrane for organic and pathogenic contaminants removal: from design to application

Surface modification of polyester fabric using plasma-dendrimer for robust immobilization of glucose oxidase enzyme

Influence of remote plasma on PEDOT:PSS‐coated carbon felt for improved activity of glucose oxidase

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