Activity of glutathione-dependent enzymes in spermatozoa in patients with pathospermia

Onufrovych, O. K.; Фафула, Р. В.; Nakonechnyi, Y.A.; Vorobets, D. Z.; Iefremova, U. P.; Воробец, З. Д.

doi:10.11603/mcch.2410-681x.2016.v0.i4.7246

Cited by 4 publications

(5 citation statements)

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“…The intensity of free radical reactions and processes depends on the concentration of oxygen and the functioning of the enzymatic and nonenzymatic antioxidant systems. In our previous studies (Onufrovych et al, 2016;Fafula et al, 2017) we found depletion of antioxidant systems in particular inhibition of glutathione peroxidase, glutathione reductase and glutathione transferase in spermatozoa of infertile men. Despite the fact that the enzymatic component of the antioxidant protection system is more sensitive to changes in prooxidant-antioxidant balance than nonenzymatic, both systems are closely related.…”

Section: Discussionmentioning

confidence: 74%

“…Thus, the changes in GSH content and activities of enzymes of its metabolism found in sperm cells (Onufrovych et al, 2016;Fafula et al, 2017) in patients with pathospermia may be interpreted as a response to development of oxidative stress. Decrease in intracellular GSH level and inhibition of glutathione peroxidase leads to overproduction of ROS which results in oxidative stress and injury in sperm cells.…”

Section: Discussionmentioning

confidence: 99%

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Glutathione content in sperm cells of infertile men

Фафула

Onufrovych

Iefremova

et al. 2017

Regul. Mech. Biosyst.

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Hyperproduction of reactive oxygen species can damage sperm cells and is considered to be one of the mechanisms of male infertility. Cell protection from the damaging effects of free radicals and lipid peroxidation products is generally determined by the degree of antioxidant protection. Glutathione is non-enzymatic antioxidant which plays an important protective role against oxidative damages and lipid peroxidation. The aim of the present work is to determine the content of reduced and oxidized glutathione in sperm cells of infertile men. Semen samples from 20 fertile men (normozoospermics) and 72 infertile patients (12 oligozoospermics, 17 asthenozoospermics, 10 oligoasthenozoospermics and 33 leucocytospermic) were used. The total, oxidized (GSSG) and reduced (GSH) glutathione levels were measured spectrophotometrically. The levels of total glutathione were significantly lower in the spermatozoa of patients with oligozoo-, asthenozoo- and oligoasthenozoospermia than in the control. Infertile groups showed signiﬁcantly decreased values of reduced glutathione in sperm cells vs. fertile men, indicating an alteration of oxidative status. The oxidized glutathione levels in sperm cells of infertile men did not differ from those of normozoospermic men with proven fertility. The GSH/GSSG ratio was signiﬁcantly decreased in the oligo-, astheno- and oligoasthenozoospermic groups compared to the normozoospermic group. In patients with leucocytospermia the GSH/GSSG ratio was lower but these changes were not significant. In addition, glutathione peroxidase activity in sperm cells was decreased in patients with oligozoo-, astenozoo-, oligoastenozoospermia and with leucocytospermia. The most significant changes in glutathione peroxidase activity were observed in infertile men with leucocytospermia. Decreased GSH/GSSG ratio indicates a decline in redox-potential of the glutathione system in sperm cells of men with decreased fertilizing potential. Redistribution between oxidized and reduced forms of glutathione can be caused by depletion of intracellular stores of glutathione and intensification of lipid peroxidation processes. This leads to increased production of reactive oxygen species, further depletion of antioxidant pools and disturbances of structure and function of spermatozoa. Our results indicate that the evaluation of reduced glutathione level and GSH/GSSG ratio in sperm cells of infertile men can be helpful in fertility assessment.

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Section: Discussionmentioning

confidence: 74%

Section: Discussionmentioning

confidence: 99%

Glutathione content in sperm cells of infertile men

Фафула

Onufrovych

Iefremova

et al. 2017

Regul. Mech. Biosyst.

Self Cite

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“…Thus, it has been shown that the concentration of MDA in sperm is largely related to the number of immobile sperm [10,11]. A number of researchers [8,9,13,15,16] has also identified elevated levels of MDA in pathospermia. In our study, we also found significantly higher lеvels of MDA in seminal plasma and sperm from abnormal groups (including oligozoospermia, asthenozoospermia, oligoastenozoospermia, leukocytospermia, and nonobstructive azoospeermia).…”

Section: характеристика оксидативного стресу та неензиматичної ланки ...mentioning

confidence: 99%

Сharacteristics of oxidative stress and non-enzymatic link of the glutathione system in sperm plasma and spermatozoa in men with different fertilization potential

VOROBETS¹

2022

Eksp.klin.fiziol.biohim

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Section: характеристика оксидативного стресу та неензиматичної ланки ...mentioning

confidence: 99%

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2022

Eksp.klin.fiziol.biohim

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Activity of glutathione-dependent enzymes in spermatozoa in patients with pathospermia

Cited by 4 publications

References 9 publications

Glutathione content in sperm cells of infertile men

Glutathione content in sperm cells of infertile men

Сharacteristics of oxidative stress and non-enzymatic link of the glutathione system in sperm plasma and spermatozoa in men with different fertilization potential

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