Activity of glutathione S-transferase in Spodoptera exigua larvae exposed to cadmium and zinc in two subsequent generations

Kafel, Alina; Bednarska, Katarzyna; Augustyniak, Maria; Witas, Ilona; Szulińska, Elżbieta

doi:10.1016/s0160-4120(02)00111-3

Cited by 31 publications

(16 citation statements)

References 6 publications

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“…40 The effects may be observed in a non-liner manner in subsequent generations. 41 Similarly report Kafel et al 42 on different effect of Cd (44 and 66 µg/g dry weight of diet) and Zn (200 µg/g dry weight of diet) in fat body of S. exigua showing significantly increses at higher concentration. In our results GST activity of metal treated Spodoptera litura midgut tissues shows significantly increased activity in Cd 0.44 mg, Pb 9.6 mg, Pb 4.8 mg, as compared to control.…”

Section: Discussionmentioning

confidence: 71%

Effect of Cadmium and Lead Exposure on Tissue Specific Antioxidant Response in Spodoptera litura

Suganya

Karthi

Shivakumar

2016

FRA

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Introduction: Oxidative stress in insects may result from an imbalance of oxidants and antioxidants under a significant impact of metals. Reactive oxygen species (ROS), such as superoxide, hydrogen peroxide, and hydroxyl radical, are generated from normal metabolic processes in all oxygen-utilizing organisms. In the present study was designed to the impact of heavy metal (Cadmium and Lead) toxicity and its free radical scavenging enzymes in Spodoptera litura larvae. Methods: Heavy metals were administered using artificial diet method having different concentrations. The metal toxicity was observed after 24 and 48 hrs post treatment. In antioxidant parameters were SOD, CAT, POX, GST, LPO, ACP and AKP were assessed. In additionally metal accumulation analysis using ICP-MS. Results: In particular, after exposure to diets containing environmentally relevant concentration (Cd 0.3 mg, Cd 0. 44 mg, Cd 0.50 mg or Pb 0.48 mg, Pb 4.8 mg, Pb 9.6 mg) at 48 hrs changes in the activity of antioxidant enzymes were measured in larvae. The results showed that compared with control, the based on concentration significantly increased of antioxidant enzymes activities. In ICP-MS analysis results shows based on the concentration Pb 9.6 mg, 325.61 ppm/mg -1 and Cd 0.44 mg, 36.5 ppm/mg -1 will be increased. Conclusion: In conclusion antioxidant systems of S. litura were altered by Cd and Pb exposure as determined by measuring in the enzymes activities. All of Cd and Pb result in strong oxidative stress and toxicity to S. litura larvae. Based on the results we suggest that metal toxicity and metal accumulation of insects influenced by Cadmium chloride and Lead nitrate in S. litura larvae.

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Section: Discussionmentioning

confidence: 71%

Effect of Cadmium and Lead Exposure on Tissue Specific Antioxidant Response in Spodoptera litura

Suganya

Karthi

Shivakumar

2016

FRA

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“…Similarly Adamski et al (2003) and Yu (2004) found fluctuations in the activities of different enzymes in fat bodies after the exposure of insects to different chemicals. Fat bodies are supposed to be sensitive to toxicity and play a vital role in the detoxification of insecticides with increased enzyme activities, as Kafel et al (2003) (Timmins and Reynolds, 1992). Enhanced activity of antioxidant enzymes play an important role in the insect immune system and can be a cause of resistance.…”

Section: Discussionmentioning

confidence: 99%

Study of destruxin B and tea saponin, their interaction and synergism activities with Bacillus thuringiensis kurstaki against Spodoptera exigua (Hübner) (Lepidoptera: Noctuidae)

Rizwan-ul-Haq¹,

Hu²,

Mei³

et al. 2009

Appl. Entomol. Zool.

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This study reveals the individual and synergistic effects of destruxin B (DB) (mycotoxin from Metarhizium anisoplae), tea saponin (Ts) and Bacillus thuringiensis (Bt) against Spodoptera exigua. DB, Ts and Bt reduced the growth of neonate larvae by up to 91.30, 89.17 and 77.17%. EC 50 values of DB against 4th and 5th instars were 0.17 and 0.22 mg ml Ϫ1, 0.35 and 0.41 mg ml Ϫ1 against 4th and 5th instars for Ts and 0.0031 and 0.0035 mg ml Ϫ1 after being treated with Bt. The synergism of DB and Ts with Bt resulted in the increased efficiency of these chemicals as mortality percentages significantly increased up to 94.2% with DBϩTsϩBt followed by DBϩBt with 91.99% and were significantly higher than individual treatments of Bt 65.81% and Ts 76.66%. Nutritional analysis revealed the increased toxicity of DB and Ts in combination with Bt, but DBϩBt showed higher efficiency with minimal relative consumption rate (RCR) 2.69 mg/mg/day, relative growth rate (RGR) 1.33 mg/mg/day and efficiency of conversion of ingested food (ECI) 35.77%, respectively. Changes in antioxidant enzymes, superoxidase dismutase (SOD) and catalases (CAT), were noticed to some extent over different exposure times in all treatments. The highest SOD activity was observed in individual DB treatment, while individual Bt treatment caused the highest variation in CAT values. In all treatments, SOD and CAT values fell sharply on the final day except for individual Bt treatment, with which the values were relatively stable on the final day.

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“…Exposure of fish to heavy metals has been shown to compromise several aspects of humoral and cell-mediated immunity and many enzymatic activities (Lemaire-Gony et al 1995). Cd, in particular, has been shown to affect phase I (cytochrome P450) (Viarengo et al 1997a) and phase II [glutathione-S-transferase (GST)] enzymes (Kafel et al 2003). GSTs are essential components of the cellular antioxidant defence system, as they catalyze the conjugation of GSH to several dangerous compounds (Arrigo 1999;Rahaman et al 1999).…”

Section: Introductionmentioning

confidence: 99%

Acute effects of cadmium on liver phase I and phase II enzymes and metallothionein accumulation on sea bream Sparus aurata

Bouraoui¹,

Bannı

Ghedira³

et al. 2007

Fish Physiol Biochem

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This research was designed to study Sparus aurata (sea bream) biotransformation and detoxification responses to acute exposure to cadmium (Cd). Sexually immature gilthead sea bream were treated by intraperitoneal injection of Cd chloride (200 microg kg(-1)) for 6, 12, 24 and 48 h. Cd accumulation was quantified in sea bream liver by graphite furnace atomic absorption spectroscopy after the various exposure periods. The following biological responses were measured: (1) ethoxyresorufin-O-deethylase (EROD) activity as phase I biotransformation parameter, (2) liver glutathione-S-transferase (GST) activity as a phase II conjugation enzyme and metallothionein (MT) content as specific response to Cd contamination. Cd bioaccumulation in the liver resulted in an increasing uptake up to 10.3 microg g(-1) wet weight after 48 h of exposure. EROD showed a significant activation only after 6 h exposure and a return to control levels after 12 h. GST revealed significant activation starting from 12 h exposure. MT accumulation in liver showed the same behavior as GST activation.

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Activity of glutathione S-transferase in Spodoptera exigua larvae exposed to cadmium and zinc in two subsequent generations

Cited by 31 publications

References 6 publications

Effect of Cadmium and Lead Exposure on Tissue Specific Antioxidant Response in Spodoptera litura

Effect of Cadmium and Lead Exposure on Tissue Specific Antioxidant Response in Spodoptera litura

Study of destruxin B and tea saponin, their interaction and synergism activities with Bacillus thuringiensis kurstaki against Spodoptera exigua (Hübner) (Lepidoptera: Noctuidae)

Acute effects of cadmium on liver phase I and phase II enzymes and metallothionein accumulation on sea bream Sparus aurata

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