Acute effects of cadmium on liver phase I and phase II enzymes and metallothionein accumulation on sea bream Sparus aurata

Bouraoui, Zied; Bannı, Mohamed; Ghedira, Jihene; Clérandeau, Christelle; Guerbej, Hamadi; Narbonne, Jean‐François; Boussetta, Hamadi

doi:10.1007/s10695-007-9177-y

Cited by 45 publications

(17 citation statements)

References 47 publications

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“…Moreover, the data obtained from the study are supported by other several findings (Ahmad et al 2000;Pandey et al 2001;Ali et al 2004;Nwani et al 2010) which show that C. punctatus used in our experiment are original fish species. Our results are also supported by some other investigators' findings (Atli et al 2006;Asagba et al 2008;Bouraoui et al 2008;Soares et al 2008Osman et al 2009) who worked on cadmiuminduced oxidative stress in various fish species.…”

Section: Discussionsupporting

confidence: 92%

Assessment of tissue-specific effect of cadmium on antioxidant defense system and lipid peroxidation in freshwater murrel, Channa punctatus

Dabas

Nagpure

Kumar

et al. 2011

Fish Physiol Biochem

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The effects of different concentrations of cadmium chloride on the extent of lipid peroxidation (LPO) and alterations in the antioxidant enzyme activities were studied in liver, kidney and gill tissues of freshwater murrel, Channa punctatus. The fish specimens were exposed to 6.7, 13.4 and 20.1 mg l(-1) sublethal concentrations of cadmium chloride and the oxidative stress was assessed after 24, 48, 72 and 96 h post-exposure. The biomarkers selected for the study were thiobarbituric acid reactive substances for assessing the extent of lipid peroxidation and antioxidant defense system such as reduced glutathione (GSH), glutathione reductase (GR), glutathione peroxidase (GP(X)), glutathione-S-transferase (GST), catalase (CAT) and superoxide dismutase (SOD) activities. In general, the cadmium exposure elevated the LPO in subject tissues of treated group and modulated the activities of GPx, GST, SOD, CAT, GR and level of GSH after given exposure as compared to the control. All enzymes activities, except CAT (in kidney and gills), and amount of LPO elevated significantly (P < 0.05) in treated group with respect to control in all tissues, while significant difference was not observed between the exposed concentrations and within exposure duration. The results indicated that increase in LPO level and the fluctuation in antioxidant defense system in fish could be due to cadmium-induced increase in the production of reactive oxygen species (ROS). The potential role of these parameters as biomarkers of heavy metal pollution in aquatic system is discussed.

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Section: Discussionsupporting

confidence: 92%

Assessment of tissue-specific effect of cadmium on antioxidant defense system and lipid peroxidation in freshwater murrel, Channa punctatus

Dabas

Nagpure

Kumar

et al. 2011

Fish Physiol Biochem

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“…GST catalyzes conjugation of many xenobiotics to the tripeptide glutathione while GPx provides optimal protection against oxidative stress (Hermes-Lima 2004) by scavenging H 2 O 2 or ROOH, and has been considered to be complementary to CAT (Halliwell and Gutteridge 1999). In the present study, GPx and GST activity increased significantly in gills with increased concentration and exposure duration, which was consistent with most investigations (Atif et al 2005;Bouraoui et al 2008), suggesting that GPx and GST activity could be elevated to resist the Cd-induced toxicity. The positive correlation between GSH and both enzymes (GPx and GST) also shows that increased amount of GSH as well as elevated activity of GPx and GST in Cd-treated fish groups may protect biological systems from oxidative stress.…”

supporting

confidence: 87%

Investigation of Cadmium-Induced Genotoxicity and Oxidative Stress Response in Indian Major Carp,Labeo rohita

Dabas

Nagpure

Mishra

et al. 2013

Human and Ecological Risk Assessment: An International Journal

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We investigated genotoxicity and oxidative stress in the gills of Labeo rohita exposed to 33.6, 67.1, and 100.6 mg L -1 of cadmium chloride at 96 h. Genotoxicity was assessed using single cell gel electrophoresis whereas oxidative stress was monitored through lipid peroxidation induction and antioxidant response parameters, namely reduced glutathione (GSH), glutathione peroxidase, glutathione-S-transferase, superoxide dismutase, and catalase (CAT) activities. Significant (p < .05) effect of both concentration and time of exposure was observed on the extent of DNA damage in treated fish. Similarly, malondialdehyde content, level of GSH, and activities of antioxidant enzymes were significantly elevated in treated groups, except CAT. The increased DNA damage and lipid peroxidation (LPO) content along with fluctuation in antioxidant defense system in fish indicated the interaction of cadmium (Cd) with DNA repair processes and production of reactive oxygen species. Thus, Cd is liable for induction of LPO, alteration of antioxidant defenses, and DNA damage in gills of L. rohita.

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“…In fish, a number of studies have described occurrence and xenobiotic induction of CYP1A in the liver (Stegeman and Hahn 1994;Sarasquete and Segner 2000;Gravato and Santos 2002;Bouraoui et al 2008). However, xenobiotic induction of CYP1A and GST in hepatic tissues and their relation with DNA damage after an acute exposure is less studied.…”

Section: Discussionmentioning

confidence: 99%

Acute effects of benzo[a]pyrene on liver phase I and II enzymes, and DNA damage on sea bream Sparus aurata

Bannı¹,

Bouraoui²,

Ghedira³

et al. 2008

Fish Physiol Biochem

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In the present study biotransformation and detoxification responses to acute exposure to the polycyclic aromatic hydrocarbons benzo[a]pyrene (B[a]P) were investigated in the liver of Sparus aurata (sea bream). Sexually immature gilthead sea bream were treated by intraperitoneal injection of B[a]P (20 mg kg(-1)) for 6, 12, 24, and 48 h. B[a]P accumulation was quantified in sea bream liver by mean of gas phase chromatography (GPC-MS) after the various exposure periods. The following biological responses were measured: (1) ethoxyresorufin-O-deethylase (EROD) activity, as a phase I biotransformation parameter; (2) liver glutathione S-transferase (GST) activity as a phase II conjugation enzyme. DNA damage was assessed over time using the single-cell gel electrophoresis comet assay. B[a]P bioaccumulation in the liver resulted in a biphasic curve with an increasing uptake up to 5.55 +/- 0.67 microg g(-1) dry weight after only 6 h exposure and 4.67 +/- 0.68 microg g(-1) dry weight after 48 h exposure. EROD activity showed a nonsymmetrical bell-shaped kinetic with a maximum at 24 h and lower but significant activities at 12 and 48 h with respect to control animals. Hepatic GST activities were only significant after 48 h exposure. Comet assay showed an increase in liver cells DNA damage with a maximum after 48 h exposure reaching up to 12.17 % DNA in the tail.

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Acute effects of cadmium on liver phase I and phase II enzymes and metallothionein accumulation on sea bream Sparus aurata

Cited by 45 publications

References 47 publications

Assessment of tissue-specific effect of cadmium on antioxidant defense system and lipid peroxidation in freshwater murrel, Channa punctatus

Assessment of tissue-specific effect of cadmium on antioxidant defense system and lipid peroxidation in freshwater murrel, Channa punctatus

Investigation of Cadmium-Induced Genotoxicity and Oxidative Stress Response in Indian Major Carp,Labeo rohita

Acute effects of benzo[a]pyrene on liver phase I and II enzymes, and DNA damage on sea bream Sparus aurata

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