Investigation of Cadmium-Induced Genotoxicity and Oxidative Stress Response in Indian Major Carp,Labeo rohita

Abstract

We investigated genotoxicity and oxidative stress in the gills of Labeo rohita exposed to 33.6, 67.1, and 100.6 mg L -1 of cadmium chloride at 96 h. Genotoxicity was assessed using single cell gel electrophoresis whereas oxidative stress was monitored through lipid peroxidation induction and antioxidant response parameters, namely reduced glutathione (GSH), glutathione peroxidase, glutathione-S-transferase, superoxide dismutase, and catalase (CAT) activities. Significant (p < .05) effect of both concentration and time of exposure was observed on the extent of DNA damage in treated fish. Similarly, malondialdehyde content, level of GSH, and activities of antioxidant enzymes were significantly elevated in treated groups, except CAT. The increased DNA damage and lipid peroxidation (LPO) content along with fluctuation in antioxidant defense system in fish indicated the interaction of cadmium (Cd) with DNA repair processes and production of reactive oxygen species. Thus, Cd is liable for induction of LPO, alteration of antioxidant defenses, and DNA damage in gills of L. rohita.

Effects of Cadmium Chloride and Glyphosate on Antioxidants as Biochemical Biomarkers in Nile Tilapia

“…Also, significant reduction in serum GSH, increase in SOD, MDA of Nile tilapia via exposure period 96h-LC 50 concentration [44]. Gills of Labeo rohita exposed to 33.6, 67.1, and 100.6 mg/l of cadmium chloride at 96 hrs led to oxidative stress where activity of LPO increased in all treated groups but in case of catalase decreased [45]. CAT activity has been inhibited in fish species exposed to pesticides such as deltamethrin [23].…”

Expression of the PEPT1, CAT, SOD2 and GPX1 genes in the zebrafish intestine supplemented with methionine dipeptide under predation risk

“…Therefore, we assume that after 20 days under predation stress, there was excessive H 2 O 2 formation. Previous studies showed a reduction in Sod, Cat and Gpx activities in fish with increasing concentrations of chemical agents (Dabas et al, 2014;Yadav et al, 2015;Yang et al, 2014). These results suggest the existence of a threshold concentration and exposure time to a stressing agent above which enzyme activity is significantly decreased.…”

Assessment of Oxidative Stress in Zebrafish (Danio rerio) Exposed to Fenvalerate: Analysis of Antioxidant Enzyme Gene Expression and Enzyme Activity

“…This is thought to be a re ex developed in sh to adapt to oxidative stress. Studies conducted earlier indicate that in sh, the activities of enzymes such as SOD, CAT, and GPX tend to decline as the concentrations of chemical substances increase (Dabas et al 2014;Yadav et al 2015). Dabas et al (2014) conducted a study investigating oxidative stress in the muscles of Nile Tilapia sh.…”

“…Studies conducted earlier indicate that in sh, the activities of enzymes such as SOD, CAT, and GPX tend to decline as the concentrations of chemical substances increase (Dabas et al 2014;Yadav et al 2015). Dabas et al (2014) conducted a study investigating oxidative stress in the muscles of Nile Tilapia sh. The study revealed that when certain chemical agents were introduced, there was a notable reduction in the activities of enzymes like SOD, CAT, GR, GPx, and GST in the exposed groups.…”