Activity of immobilized lipase from Candida antarctica (Lipozyme 435) and its performance on the esterification of oleic acid in supercritical carbon dioxide

Santos, Philipe dos; Rezende, Camila A.; Martínez, Julián

doi:10.1016/j.supflu.2015.08.011

Cited by 40 publications

(19 citation statements)

References 56 publications

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“…Furthermore, some researchers suggest that pressurized CO 2 forms covalent complexes with free amine groups on the surface of enzymes. These complexes can remove the charges of residues of lysine, which influences the enzyme activity …”

Section: Resultsmentioning

confidence: 99%

“…It is important to point out that immobilization of CALB in polyurethane was shown to be effective in terms of increasing residual activity. Santos, Rezende and Martínez evaluated the activation of CALB immobilized in a macroporous anionic resin. Activation was performed in supercritical CO 2 , but the residual activity reduced whether compared to fresh activity.…”

Section: Resultsmentioning

confidence: 99%

“…This conversion of oleic acid in esters was corroborated by other studies. For example, Santos, Rezende and Martínez obtained an oleic acid conversion yield of 67.7% when using lipase immobilized on macroporous anionic resin as a catalyst in reactions performed at 50 °C. The esterification reactions were conducted in supercritical CO 2 medium for 120 min.…”

Section: Resultsmentioning

confidence: 99%

“…Recently, scientists started using pressurized fluids in subcritical or supercritical states for activating enzymes . The objective is to maintain or to increase the enzymatic activity under high‐pressure conditions.…”

Section: Introductionmentioning

confidence: 99%

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Activation of Candida antarctica lipase B in pressurized fluids for the synthesis of esters

Nyari

Zabot

Zamadei

et al. 2017

J of Chemical Tech & Biotech

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BACKGROUND The objective of this work was to evaluate the activation of Candida antarctica lipase B (CALB) in pressurized CO2 or liquefied petroleum gas (LPG) and, thereafter, to evaluate the synthesis of esters using activated CALB as a biocatalyst. Before activation, CALB was immobilized in polyurethane. For the esterification reactions, mechanical or ultrasonic agitations were studied. The stability of CALB after reuse cycles was also evaluated. RESULTS Before activation, the enzymatic activity of immobilized CALB was 789 U g‐1. After activation in pressurized CO2 and LPG, the activities increased to 2486 U g‐1 and 1413 U g‐1, respectively. Consequently, the residual activities of CALB after activation in pressurized CO2 and LPG were 315% and 179%, respectively. For the best condition of reaction under ultrasonic agitation, oleic acid conversion was 51.2% when using non‐activated CALB. Otherwise, oleic acid conversions were 66.5% and 77.1% when using CALB activated in LPG and CO2, respectively. This biocatalyst maintained more than 80% of its residual activity when reused for up to 13 cycles. CONCLUSION Activation of CALB in pressurized CO2 provided enzymatic activities 1.76 times higher than activation in pressurized LPG did. Immobilized CALB had good performance as a stable biocatalyst for esterification reactions under ultrasonic agitation. © 2017 Society of Chemical Industry

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Activation of Candida antarctica lipase B in pressurized fluids for the synthesis of esters

Nyari

Zabot

Zamadei

et al. 2017

J of Chemical Tech & Biotech

View full text Add to dashboard Cite

show abstract

“…In these experiments the temperature and pressure were fixed at 40 • C and 10 MPa, respectively. In these conditions the immobilized lipases have shown their highest residual activities [24][25][26]. Moreover, the stirring rate was fixed in a high value to ensure that the reaction would be kinetically controlled and the inter-particle diffusion and external mass transfer effects were negligible [27,28].…”

Section: Effect Of the Enzyme Concentrationmentioning

confidence: 99%

Synthesis of eugenyl acetate by enzymatic reactions in supercritical carbon dioxide

Santos

Zabot

Meireles

et al. 2016

Biochemical Engineering Journal

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Supercritical transesterification route for biodiesel production: Effect of parameters on yield and future perspectives

Singh

Kumar

Gautam

2021

Env Prog and Sustain Energy

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Catalyst‐free biodiesel production through supercritical transesterification is faster with the high conversion and a more environment‐friendly route than conventional catalyst transesterification. Improving and exploring the new possibilities in this alternative biodiesel production method by including newly available technology will lead us to a more sustainable future. In this paper, supercritical transesterification of biodiesel production has been reviewed in detail. The different parameters of this method, such as temperature, pressure, molar ratio, alcohol types, and residence time which affect the yielding and quality of fuel, have been reviewed. Besides, recycling the energy and suitable feedstock for this method has also been explored to reduce the intensive energy demand and operating cost. Furthermore, light has also thrown on co‐solvents effect on this method to reduce the extreme reaction conditions required. At last, the review concludes future recommendations in supercritical transesterification along with the challenges in energy integration and recycling.

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Activity of immobilized lipase from Candida antarctica (Lipozyme 435) and its performance on the esterification of oleic acid in supercritical carbon dioxide

Cited by 40 publications

References 56 publications

Activation of Candida antarctica lipase B in pressurized fluids for the synthesis of esters

Activation of Candida antarctica lipase B in pressurized fluids for the synthesis of esters

Synthesis of eugenyl acetate by enzymatic reactions in supercritical carbon dioxide

Supercritical transesterification route for biodiesel production: Effect of parameters on yield and future perspectives

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