Activity of Naturally Derived Antimicrobial Peptides against Filamentous Fungi Relevant for Agriculture

Abstract

The search for environmentally biocompatible and cost-effective methods to control filamentous fungi in agriculture is becoming increasingly urgent. In vitro antimicrobial activity of three synthetic peptides was investigated against some filamentous fungi with agricultural relevance. The peptides were an analog of Temporin called Temporizina, a fragment from Pleurocidin termed Plc-2, and a peptide identified from sesame seeds named Pses3. Antimicrobial activity of these peptides towards filamentous fungi has not been previously reported. Seven plant pathogenic or mycotoxigenic fungal species, isolated from plant tissues were assayed: Alternaria solani, Colletotrichum gloesporioides, Fulvia fulvum, Fusarium oxisporum, Aspergillus niger, A. ochraceus and Penicillium digitatum. Values of Minimum Inhibitory Concentration (MIC) and Minimum Fungicidal Concentration (MFC) were determined and compared with the commercially available fungicide Captan as a positive control. The peptides showing greatest inhibition were Pses3 and Plc-2 and C. gloesporioides was the most sensitive of the evaluated fungi. The MIC values for Plc-2 and Pses3 peptides ranged from 0.64 µM to 10.25 µM. These values were much lower than those observed for Captan, suggesting the potential of these peptides as fungicides. In particular, Pses3 is a novel peptide derived from sesame seeds not reported in databases.

Studying of cellular interaction of hairpin‐like peptide EcAMP1 from barnyard grass (Echinochloa crusgalli L.) seeds with plant pathogenic fungus Fusarium solani using microscopy techniques

“…Plant diseases cause significant economic losses in the agricultural sector (Larrañaga et al ., ). In this regard, much attention has been drawn to the development of efficient strategies to protect plants from different plant pathogenic organisms (Butts and Krysan, ).…”

Direct identification of fungi associated with indoor and outdoor ornamental plants by utilizing PCR assay

“…Many fungal infections can survive in the soil as saprophytes feeding on dead organic debris, or as dormant spores resistant to extreme climatic conditions such as heat or dehydration (Schippers and Gams, 1979). Phytopathogenic fungi are an important infectious agent in plants, as more than 70% of important crop diseases caused by fungi (Agrios and Pichat, 2005); (Larrañaga et al, 2012). Ornamental plants have worldwide importance due to their vital role in different communities.…”