2001
DOI: 10.1046/j.1198-743x.2001.00298.x
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Activity of quinupristin–dalfopristin in invasive isolates of Streptococcus pneumoniae from Italy

Abstract: Eighty-five recent isolates of Streptococcus pneumoniae from patients with invasive disease were examined for their susceptibility to erythromycin, clindamycin, penicillin and quinupristin-dalfopristin by E test. A novel duplex PCR assay was used to detect the presence of the erm(B) or mef(A) genes in all of the erythromycin-resistant isolates. All of the strains tested were susceptible to the combination quinupristin-dalfopristin, regardless of their susceptibility to penicillin or to erythromycin. By duplex … Show more

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Cited by 5 publications
(3 citation statements)
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“…In brief, selected isolates were subject to PCR using primers for the genes int and xis , and tnpR and tnpA to detect the presence of transposons in the Tn 916 and Tn 917 families respectively [29]. Depending on their resistance gene profile, some isolates positive for only Tn916 were subject to PCR using the following primer pairs: SG1 and LTf [30] to substantiate the presence of Tn 2009 or Tn 2010 with a 1 kb PCR product, EB2 [31] and TN2 [32] to confirm Tn 2010 with a 3.3 kb PCR product, and J12 and J11 to detect and differentiate Tn 6002 (3.6 kb PCR product) from Tn 6003/ Tn 1545 (7.9 kb PCR product) [33]. Isolates positive for both transposon families were subject to PCR using primers J12 and J11 to detect Tn 3872 with an 800 bp PCR product.…”
Section: Methodsmentioning
confidence: 99%
“…In brief, selected isolates were subject to PCR using primers for the genes int and xis , and tnpR and tnpA to detect the presence of transposons in the Tn 916 and Tn 917 families respectively [29]. Depending on their resistance gene profile, some isolates positive for only Tn916 were subject to PCR using the following primer pairs: SG1 and LTf [30] to substantiate the presence of Tn 2009 or Tn 2010 with a 1 kb PCR product, EB2 [31] and TN2 [32] to confirm Tn 2010 with a 3.3 kb PCR product, and J12 and J11 to detect and differentiate Tn 6002 (3.6 kb PCR product) from Tn 6003/ Tn 1545 (7.9 kb PCR product) [33]. Isolates positive for both transposon families were subject to PCR using primers J12 and J11 to detect Tn 3872 with an 800 bp PCR product.…”
Section: Methodsmentioning
confidence: 99%
“…Macrolide-nonsusceptible (clarithromycin MIC of 0.5 µg/mL or greater) respiratory (n=97) and bacteremic (n=27) S pneumoniae isolates were analyzed for the presence of ermB and/or mefA genes using a multiplex polymerase chain reaction assay as described by Monaco et al (13) and Pantosti et al (14). mefA, ermB and dual mefA and ermB positive controls as well as ATCC 49619 S pneumoniae (susceptible to macrolides; negative control) were used.…”
Section: Genotypingmentioning
confidence: 99%
“…Pneumococcal isolates were examined by PCR for the presence of the resistance determinants erm(B), mef(A), tet(M), cat, and aphA-3. A duplex PCR was used to amplify the genes erm(B) and mef(A) with primer pairs EB1-EB2 and ME1-ME2, as described previously (22). Internal fragments of the tet(M) and cat genes were amplified with primer pairs TETMd-TETMr and CATd-CATr, respectively, described by Marchese et al (15).…”
Section: Methodsmentioning
confidence: 99%