Activity of two Exometabolites produced by Escherichia coli on the Synthesis of Pyocyanin

Abstract

The secretion of metabolites with antimicrobial activity is one of the strategies employed by bacteria to respond to negative stimuli promoted during interspecies competition. In a long -term stationary phase. Pseudomonas aeruginosa and Escherichia coli can synthesize diffusible exometabolites whose action is to mutually inhibit the exposed cells, guaranteeing the balance of both populations in a certain site. The P. aeruginosa may have an advantage in that it produces pyocyanin. Howeve r, the excretion of indole and acetate by E. coli may reduce this advantage. This work aims to detect the influence of different concentrations of these two exometabolites on the synthesis of pyocyanin in two wild isolates of P. aeruginosa. After incubatio n under shaking for 72 h at 29°C, reduction of up to 50% of the concentration of pyocyanin in the presence of indole was observed. On the other hand, no change was observed in the production of the pigment with the acetate, alone or when in combination with concentrations of less than 0.5 mM indole. It reduced the inhibitory effect of the compound, reflecting an increase in pyocyanin production of more than 20%. The results contribute to help understanding the ecological mechanisms of competition between t he two species.

A purified and lyophilized Pseudomonas aeruginosa derived pyocyanin induces promising apoptotic and necrotic activities against MCF-7 human breast adenocarcinoma

“…The designed modified semisynthetic (MS) media for pyocyanin yield enhancement led to increasing pyocyanin yield up to 6 μg/ml [ 31 ]. The study established by [ 32 ] showed a pyocyanin reduction by indole addition and no change was detected in the pyocyanin yield with acetate addition.…”

Ensaio rápido, simples e confiável para detectar o potencial degradador de triazinas por Pseudomonas aeruginosa hidrocarbonoclásticas / Fast, simple and reliable assay to detect the degrading potential of triazines by hydrocarbonoclastic Pseudomonas aeruginosa

“…Sugere-se apenas que o resultado mais rápido de TGC02, comparada ao isolado TGC04, ocorreu em função do pigmento produzido. A piocianina pode ser sintetizada em situações de estresse sofrido pela P. aeruginosa, incluindo redução de nutrientes, presença de competidores e exposição à moléculas complexas (ARRUDA et al, 2019). Uma das funções mais importantes da piocianina está relacionada à mitigação do estresse oxidativo, garantindo a persistência de P. aeruginosa em ambientes mais hostis (GONÇALVES; VASCONCELOS, 2021).…”

Antifungal Coating Based on Pyocyanin Nanoparticles (Np-Pyo)

“…Pyocyanin is a secondary metabolite involved in natural ecological phenomena of antibiosis and amensalism of P. aeruginosa against its competitors (Jameel et al, 2017;Ghoul & Mitri, 2016). Depending on the concentration of pyocyanin, as well as the susceptibility of the organism exposed to the substance, a biocidal or biostatic effect may result (Arruda et al, 2020). Due to the antimicrobial properties and wide spectrum of action of pyocyanin (Vasconcelos et al, 2010), the molecule is a promising naturally active compound with potential and physicochemical characteristics that may be incorporated into nanosystems for the formulation of coatings.…”