Activity of type IV collagenases (MMP-2 and MMP-9) in primary pulmonary carcinomas: a quantitative analysis

Hrabec, Elżbieta; Strek, Malgorzata; Nowak, Dariusz; Greger, Janusz; M, Suwalski; Hrabec, Zbigniew

doi:10.1007/s00432-001-0320-3

Cited by 73 publications

(59 citation statements)

References 23 publications

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“…Matrix metalloproteinase-2 has been associated with tumor progression, metastasis, and poor prognosis in various different cancers, including those of pancreatic, liver, lung, renal, bladder, gastric, ovarian, and mucosal squamous cell carcinoma origins. [6][7][8][9][10][11][12][13][14][15][16][17][18][19] Matrix metalloproteinase-2 appears to play a role in the degradation of extracellular matrix components, including type IV collagen. [1][2][3] Compromise of the basement membrane, of which type IV collagen is a key component, is a vital step in invasion and metastasis.…”

Section: Discussionmentioning

confidence: 99%

“…1 Collagenase IV activity is particularly associated with basement membrane compromise. 4,6 Matrix metalloproteinase-2 overexpression has been correlated with tumor invasiveness and metas-tasis in a wide variety of cancer types, [6][7][8][9][10][11][12][13][14][15] including squamous cell carcinoma arising on mucous membranes. [16][17][18][19] A significant increase in matrix metalloproteinase-2 expression has been observed in invasive and metastatic oral squamous cell carcinoma 6,17,19 and has been associated with a shorter disease-free survival.…”

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confidence: 99%

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Metalloproteinase-2 expression correlates with aggressiveness of cutaneous squamous cell carcinomas

2004

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Matrix metalloproteinases compose a family of enzymes involved in degradation of the extracellular matrix. Tumor cells must penetrate the basement membrane and traverse the extracellular matrix in order to invade surrounding structures and metastasize to distant sites. Gelatinases, particularly gelatinase A (matrix metalloproteinase-2), demonstrate degradative activity against components of the basement membrane and may be involved in the progression of in situ squamous cell carcinoma lesions. Matrix metalloproteinase-2 overexpression has been correlated with tumor invasiveness and metastasis in a wide variety of cancer types, including squamous cell carcinoma arising on mucous membranes. However, correlation between matrix metalloproteinase-2 overexpression and the spread and prognosis of cutaneous squamous cell carcinoma has not been characterized in the literature at present. In this study, we used immunohistochemical techniques to examine the expression of matrix metalloproteinase-2 in 10 actinic keratosis, 15 in situ, 13 invasive, 13 primary (with documented metastatic disease), 11 metastatic, and 8 recurrent squamous cell carcinoma cases. We found that while the average staining intensity (scale 0-3 þ , 3 þ being strongest) of actinic keratosis and in situ lesions was not statistically significant (0.87-1.3 and 0.75-1.4, respectively), the average staining intensity of invasive squamous cell carcinomas (1.6-2.5) was significantly greater than that of actinic keratosis and in situ lesions. Likewise, while the average staining intensity of primary squamous cell carcinomas and metastatic squamous cell carcinomas was not found to be statistically significant (3.1-3.5 and 3.1-3.8, respectively), the average staining intensity of these lesions was significantly higher than that of invasive lesions. We also found that the intensity of matrix metalloproteinase-2 staining correlates with cellular atypia, inflammation, neovascularization, and the invasive tumor front, as well as tumor aggressiveness, and may play a role in the pathogenesis, invasion, and metastasis of cutaneous squamous cell carcinoma.

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Section: Discussionmentioning

confidence: 99%

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confidence: 99%

Metalloproteinase-2 expression correlates with aggressiveness of cutaneous squamous cell carcinomas

2004

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“…After 24 h, the cells were washed twice with PBS. Next, the cells were incubated in serum-free media in the presence of the above-mentioned stimulant, with or without of EPFP, for 48 h. Gelatin zymography of cell culture media samples was performed as described previously [19]. Briefly, 20 µL volumes of media were dissolved in an electrophoresis sample buffer containing sodium dodecyl sulfate (SDS) and subjected to electrophoresis in a 10% polyacrylamide gel embedded with gelatin at a concentration of 1.5 mg mL -1 in the absence of β-mercaptoethanol.…”

Section: Gelatin Zymographymentioning

confidence: 99%

Differentiated impact of procyanidins from evening primrose on human breast cancer cells

et al. 2014

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This study examines some of the biological activities of an evening primrose flavanol preparation (EPFP) against non-invasive human breast cancer cells (MCF-7). The results are compared with those obtained for highly invasive human breast cancer cells (MDAMB-231). The results show, for the first time, that EPFP reduces MCF-7 cell number, IC50 = 75 µM gallic acid equivalents/GAE for 72 h incubation, and reduces migration to 52% of the control value at 100 µM L−1 GAE. EPFP caused favorable changes in Bcl-2/Bax mRNA ratio, which rendered MCF-7 cells more sensitive to apoptosis: the number of apoptotic cells increased 2.2-fold vs. control at 100 µM GAE. Furthermore, 100 µ M L−1 GAE EPFP caused a 1.8-fold reduction in the activity of metalloproteinase-9 (MMP-9) secreted to the culture medium by MCF-7 cells. Moreover, EPFP suppressed the expression of selected genes of matrix metalloproteinases (MMPs), a proliferation marker (Ki67), and vascular endothelial growth factor (VEGF). In conclusion, the results of this study suggest that EPFP may exhibit proapoptotic, antiproliferative, antimigratory, and antimetastatic potential towards both selected human breast cancer cell lines, which is more pronounced in the case of the highly invasive MDA-MB-231 cells.

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“…31 Briefly, the extracts of tumor tissues were dissolved in electrophoresis sample buffer containing SDS and subjected to electrophoresis in a 10% polyacrylamide gel embedded with 1.5 mg/ml gelatin in the absence of b-mercaptoethanol. After electrophoresis the enzymes were renatured by incubation with 2.5% Triton X-100, and the enzyme reaction was allowed to proceed at 37°C for 21 hr.…”

Section: Gelatin and Fibrin Zymographymentioning

confidence: 99%

RNAi targeting urokinase‐type plasminogen activator receptor inhibits metastasis and progression of oral squamous cell carcinoma in vivo

Zhou

Tang

Liang

et al. 2009

Intl Journal of Cancer

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It has been admitted that urokinase-type plasminogen activator receptor (u-PAR) is overexpressed in many human malignant tumors including oral squamous cell carcinoma (OSCC) and plays an important role in a variety of cancer key cellular events as a versatile signaling orchestrator. In our study, a retroviral vector expressing u-PAR-specific siRNA was injected into OSCC xenografts of nude mice to observe its inhibitory effects on OSCC. Our data demonstrate that siRNA targeting u-PAR markedly suppressed tumor growth, reduced the expression of proliferation-related gene, Ki-67 and increased cell apoptosis, accompanying with the efficient and specific inhibition of endogenous u-PAR expression in OSCC. More importantly, the mRNA and protein expression of MMP-2, MMP-9, VEGF-C, VEGF-D and VEGFR-3 that are intimately involved in oral cancer invasion and metastasis, was simultaneously downregulated significantly as determined by quantitative real-time RT-PCR, Western blot and immunohistochemistry; and Gelatin and fibrin zymography showed that MMP-9, MMP-2 and u-PA enzymatic activities were significantly reduced in u-PAR-specific siRNA group, compared to those in control groups. In addition, the expression of MDR-1 gene related to drug resistance was obviously inhibited by silencing of u-PAR. These findings suggest that RNAi targeting u-PAR could effectively inhibit the metastasis and progression of OSCC in vivo. Thus, it may be used as a potent and specific therapy for oral cancer, especially in inhibiting and preventing cancer cell invasion and metastasis. ' UICCKey words: RNA interference; urokinase-type plasminogen activator receptor; oral squamous cell carcinoma; metastasis; cancer progression Oral squamous cell carcinoma (OSCC) accounts for about 80% of all oral malignant tumors. The 5-year survival rate for patients with OSCC was still poor, approximately 56%, despite advances in chemotherapy, radiotherapy and surgical therapy in the past 20 years.1,2 The relatively high mortality of OSCC is predominantly associated with widespread lymphatic and distant metastasis.

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Activity of type IV collagenases (MMP-2 and MMP-9) in primary pulmonary carcinomas: a quantitative analysis

Cited by 73 publications

References 23 publications

Metalloproteinase-2 expression correlates with aggressiveness of cutaneous squamous cell carcinomas

Metalloproteinase-2 expression correlates with aggressiveness of cutaneous squamous cell carcinomas

Differentiated impact of procyanidins from evening primrose on human breast cancer cells

RNAi targeting urokinase‐type plasminogen activator receptor inhibits metastasis and progression of oral squamous cell carcinoma in vivo

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