Activity Staining for Detection of Ribonucleases Using Dried Agarose Film Overlay Method After Isoelectric Focusing

Yasuda, Toshihiro; Takeshita, Hitoshi; Kishi, Koichiro

doi:10.1016/s0076-6879(01)41147-5

Cited by 6 publications

(2 citation statements)

References 37 publications

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“…Previously, we developed a zymogram method for detection of RNase in serum and urine after isoelectric focusing, allowing reproductive demonstration of isoenzyme patterns derived from the enzyme. 18 However, screening of Japanese subjects revealed only the common isoenzyme pattern, and no alternative one was encountered (data not shown), compatible with the findings of the present study. Needless to say, SNPs could be suitable for clinicalgenetic analysis such as survey of potential diseasesusceptibility genes.…”

Section: Resultssupporting

confidence: 91%

Three single nucleotide polymorphisms leading to non‐synonymous amino acid substitution in the human ribonuclease 2 and angiogenin genes exhibit markedly less genetic heterogeneity in six populations

Ueki

Takeshita

Fujihara

et al. 2008

Cell Biochemistry & Function

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Angiogenin and ribonuclease 2 (RNase 2) are members of the human RNase superfamily. Although three potential single nucleotide polymorphisms (SNPs) in these genes, which could give rise to an amino acid substitution in the protein, have been identified, relevant population data are not available, and accordingly they have not been applied to clinical-genetic analysis. For this purpose, a novel genotyping method for each SNP using the mismatched PCR-restriction fragment length polymorphism technique has been developed. Using this method, the genotype distribution of each SNP was investigated in six populations: Japanese (n ¼ 167), Korean (n ¼ 90), Mongolian (n ¼ 92), Ovambos (n ¼ 86), Turkish (n ¼ 87), and German (n ¼ 70). In all the populations, only one genotype was found in each SNP. Irrespective of differences in ethnic groups, the angiogenin and RNase 2 genes appear to exhibit markedly less genetic heterogeneity with regard to these SNPs.

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Section: Resultssupporting

confidence: 91%

Three single nucleotide polymorphisms leading to non‐synonymous amino acid substitution in the human ribonuclease 2 and angiogenin genes exhibit markedly less genetic heterogeneity in six populations

Ueki

Takeshita

Fujihara

et al. 2008

Cell Biochemistry & Function

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“…Therefore, it appears that irrespective of differences in ethnic groups, RNASE1 shows remarkably low heterogeneity of its genetic structure with regard to these SNPs. Previously, we developed a zymogram method for detection of pancreatic RNase in serum and urine after isoelectric focusing, allowing reproducible demonstration of isoenzyme patterns derived from the enzyme (Yasuda et al 2001). Screening of Japanese subjects, however, revealed only the common isoenzyme pattern, and no alternative one was encountered (Yasuda et al unpublished data), compatible with the findings of the present study.…”

Section: Gmentioning

confidence: 99%

Development of Genotyping Methods for Single Nucleotide Polymorphism in the Human Pancreatic Ribonuclease Gene (RNASE1) and Their Application to Population Studies

et al. 2008

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Two potential single nucleotide polymorphisms [SNPs; rs1804215 (G979T) and rs11545379 (G1169T)] have been identified in the human pancreatic ribonuclease, RNase 1, gene (RNASE1) that could give rise to an amino acid substitution in the protein, but relevant population data are not available. We have developed genotyping methods for each SNP using the mismatched PCR-restriction fragment length polymorphism technique. These methods are advantageous in comparison with other SNP genotyping methods because they are technically simpler and do not require specialized instruments. We applied these genotyping methods to examine the genotype distribution of each SNP in four populations, including Japanese populations living in two prefectures, an Ovambo population, and a Turkish population. In all the populations studied, however, only a single genotype for each SNP was found. Therefore, irrespective of differences in ethnic groups, RNASE1 might show markedly low heterogeneity in its genetic structure with regard to these SNPs.

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Production and characterization of murine monoclonal anti-human DNase II antibodies, and their use for immunoaffinity purification of DNase II from human liver and urine

Nakajima

Yasuda

Takeshita

et al. 2002

Biochimica et Biophysica Acta (BBA) - General Subjects

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Activity Staining for Detection of Ribonucleases Using Dried Agarose Film Overlay Method After Isoelectric Focusing

Cited by 6 publications

References 37 publications

Three single nucleotide polymorphisms leading to non‐synonymous amino acid substitution in the human ribonuclease 2 and angiogenin genes exhibit markedly less genetic heterogeneity in six populations

Three single nucleotide polymorphisms leading to non‐synonymous amino acid substitution in the human ribonuclease 2 and angiogenin genes exhibit markedly less genetic heterogeneity in six populations

Development of Genotyping Methods for Single Nucleotide Polymorphism in the Human Pancreatic Ribonuclease Gene (RNASE1) and Their Application to Population Studies

Production and characterization of murine monoclonal anti-human DNase II antibodies, and their use for immunoaffinity purification of DNase II from human liver and urine

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