Actual Ratios of Triacylglycerol Positional Isomers and Enantiomers Comprising Saturated Fatty Acids and Highly Unsaturated Fatty Acids in Fishes and Marine Mammals

Comparison of the Distribution of Unsaturated Fatty Acids at the <i>Sn</i>-2 Position of Phospholipids and Triacylglycerols in Marine Fishes and Mammals

“…We reported that the abundance ratio of PDP and PEP were null, and DHA 22:6n-3 and EPA 20:5n-3 were mainly located at the sn-3 position of TAG in marine mammals. The previous reports, which analyzed the distribution of HUFAs at the sn-1, -2, and -3 positions of TAG, also support this result 3,16,17 . This could be caused by the reaction preference of substrates for diacylglycerol acyltransferase.…”

“…However, that these fatty acids mainly locate at the sn-1,3 position of TAG in marine mammals was revealed in this study and the obtained results were the same as those of a previous report. We previously analyzed the abundance ratio among TAG isomers, such as 1,2-dipalmitoyl-3-docosahexaenoyl-sn-glycerol sn-PPD , 1,3-dipalmitoyl-2-docosahexaenoyl-sn-glycerol PDP , and 2,3-dipalmitoyl-1-docosahexaenoyl-sn-glycerol sn-DPP and 1,2-dipalmitoyl-3-eicosapentaenoyl-sn-glycerol sn-PPE , 1,3-dipalmitoyl-2-eicosapentaenoyl-sn-glycerol PEP , and, 2,3-dipalmitoyl-1-eicosapentaenoyl-sn-glycerol sn-EPP in marine mammals 16 . We reported that the abundance ratio of PDP and PEP were null, and DHA 22:6n-3 and EPA 20:5n-3 were mainly located at the sn-3 position of TAG in marine mammals.…”

Separation of Enantiomeric Triacylglycerols by Chiral‐Phase HPLC

“…The latter two are considered essential FA for humans. Chiral chromatography of TAG containing these predominantly C18 acids has been described several times [15,[25][26][27]; the most detailed study was published by Lisa and Holcapek [14], who analyzed more than 120 TAG.…”

Regioisomeric and enantiomeric analysis of triacylglycerols

“…In the case of the enantiomers it is always necessary to obtain a standard of at least one of the two possible enantiomers, because the order of elution from the column is practically impossible to predict. Just a minor modification of the stationary phase, e.g., Cl replacement for methyl (cellulose-tris-(3,5-dimethylphenylcarbamate) versus cellulose-tris-(3-chloro-4-methylphenyl carbamate)) suffices to reverse the order of elution [58].…”