Acupuncture Activates ERK-CREB Pathway in Rats Exposed to Chronic Unpredictable Mild Stress

Recent studies have demonstrated a scrutinized association of diabetes mellitus with depressive symptoms and major depression. Glycogen synthase kinase-3 (GSK-3) is a protein kinase enzyme constitutively active in non-stimulated cells and in multiple signalings. Independent lines of research provide a converging evidence for an involvement of GSK-3 in the regulation of behavior and hyperglycemia. The present study revealed that streptozotocin (STZ)-induced diabetic rats were found to show lengthened duration of immobility in the forced-swimming test (FST) and reduced locomotor and exploratory activities in the open-field test (OFT). Imipramine (15 mg/kg), Paroxetine (10 mg/kg) and lithium carbonate (36.94 mg/kg) for 14 days reduced immobility behavior in FST. Paroxetine and lithium carbonate increased the locomotor and exploratory activities, while imipramine decreased the locomotor activity in the OFT. Imipramine and lithium carbonate reduced the blood glucose level while paroxetine didn't alter it. STZ-induced diabetes increased GSK-3 gene expression which was determined using the reverse transcription-quantitative polymerase chain reaction test, while the three drugs decreased its expression. It can be concluded that lithium carbonate and imipramine can control both hyperglycemia and the associated symptoms of depression at the same time by inhibiting GSK-3 activity. On the other hand, paroxetine may only manage the depressive-like symptoms associated with diabetes through modulating the enzyme GSK-3, without changing blood glucose levels.

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“…The present findings are in accordance with the results of Lu et al [47]. Current work is not in agreement with the study of Cunha et al [48].…”

Section: Discussionsupporting

confidence: 73%

Effect of Imipramine, Paroxetine, and Lithium Carbonate on Neurobehavioral Changes of Streptozotocin in Rats: Impact on Glycogen Synthase Kinase-3 and Blood Glucose Level

Nadeem¹,

Ahmed

El-Denshary

2015

Neurochem Res

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“… Lu 2008 47 CUMS & Solitary SD rats Male 200 ± 20 8/8 mention not mention SAME EA GV20,GV29,ST36,ST40 mention 15 min 11 22d Maprotiline OFT:NC,NR; W:FW; SI:CSI. Lu 2013 48 CUMS SD rats Male 180–200 10/10 mention not mention SAME MA GV20,PC6 mention 10 min 14 28d Paroxetine OFT:NC,NR;W:FW; SI:RSI. Lu 2016 49 CUMS SD rats Male 180–200 8/8 mention not mention SAME MA GV20,PC6 mention 10 min 14 28d Fluoxetine OFT:NC,NR;W:FW; SI:CSI.…”

Section: Resultsmentioning

confidence: 99%

Acupuncture for behavioral changes of experimental depressive disorder: a systematic review and meta-analysis

Kou

Chen

et al. 2017

Sci Rep

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Acupuncture is considered to be a promising alternative therapy for depression. Nevertheless, up to now, it remains controversial on the effects exerted by acupuncture on behavioral changes in depression models. Consequently, it’s necessary to develop a systematic review and meta-analysis to assess the effect of acupuncture for model rats of depression. Ultimately, 90 studies involving 1861 models were identified. Behavioral indicators including the number of crossings (NC) and the number of rearings (NR) in open field test (OFT), the capacity of sucrose intake (CSI) and the rate of sucrose intake (RSI) in sucrose intake test (SIT), final weight (FW) and gain weight (GW) were employed as main outcomes in depression model rats. The pooled results showed that acupuncture had not less effect than western medicine on improving NC, NR, FW, GW, RSI (P > 0.05). However, the CSI improvement was poorer compared with west medicine (P < 0.05). In conclusion, acupuncture has not less effect on behavior changes than western medicine, including improving NC, NR, RSI, FW and GW in depression models.

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“…However, some results may be used as a reference. For example, in depression model rats, EA could reverse CUS induced considerable upregulation of p-ERK expression, ratio of p-ERK1/2 to ERK1/2 and the ratio of p-CREB to CREB in the hippocampus [ 47 ], or enhanced the activation of hippocampal ERK signaling pathway [ 48 ], suggesting an involvement of hippocampal ERK–CREB signaling in EAS-induced antidepressant-like effects. At the spinal level, EAS could suppress complete Freund's adjuvant- (CFA-) induced activation or phosphorylation of p38MAPK in rats with inflammatory pain [ 49 , 50 ].…”

Section: Discussionmentioning

confidence: 99%

Effect of Repeated Electroacupuncture Intervention on Hippocampal ERK and p38MAPK Signaling in Neuropathic Pain Rats

Wang

Chen

Gao

et al. 2015

Evidence-Based Complementary and Alternative Medicine

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Results of our past studies showed that hippocampal muscarinic acetylcholine receptor (mAChR)-1 mRNA and differentially expressed proteins participating in MAPK signaling were involved in electroacupuncture (EA) induced cumulative analgesia in neuropathic pain rats, but the underlying intracellular mechanism remains unknown. The present study was designed to observe the effect of EA stimulation (EAS) on hippocampal extracellular signal-regulated kinases (ERK) and p38 MAPK signaling in rats with chronic constrictive injury (CCI) of the sciatic nerve, so as to reveal its related intracellular targets in pain relief. After CCI, the thermal pain thresholds of the affected hind were significantly decreased compared with the control group (P < 0.05). Following one and two weeks' EAS of ST 36-GB34, the pain thresholds were significantly upregulated (P < 0.05), and the effect of EA2W was remarkably superior to that of EA2D and EA1W (P < 0.05). Correspondingly, CCI-induced decreased expression levels of Ras, c-Raf, ERK1 and p-ERK1/2 proteins, and p38 MAPK mRNA and p-p38MAPK protein in the hippocampus tissues were reversed by EA2W (P < 0.05). The above mentioned results indicated that EA2W induced cumulative analgesic effect may be closely associated with its function in removing neuropathic pain induced suppression of intracellular ERK and p38MAPK signaling in the hippocampus.

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Acupuncture Activates ERK-CREB Pathway in Rats Exposed to Chronic Unpredictable Mild Stress

Cited by 45 publications

References 34 publications

Effect of Imipramine, Paroxetine, and Lithium Carbonate on Neurobehavioral Changes of Streptozotocin in Rats: Impact on Glycogen Synthase Kinase-3 and Blood Glucose Level

Effect of Imipramine, Paroxetine, and Lithium Carbonate on Neurobehavioral Changes of Streptozotocin in Rats: Impact on Glycogen Synthase Kinase-3 and Blood Glucose Level

Acupuncture for behavioral changes of experimental depressive disorder: a systematic review and meta-analysis

Effect of Repeated Electroacupuncture Intervention on Hippocampal ERK and p38MAPK Signaling in Neuropathic Pain Rats

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