Acute and chronic toxicity assessment of benzylpenicillin G residue in heat-treated animal food products

Abstract

The current level of penicillin use and its persisting residue in livestock is potentially concerning; the toxicity of penicillin residue in heat-treated animal food products (HAFP) is yet to be elucidated. In this study, the acute and chronic toxicity of benzylpenicillin G (BPG) residue in HAFP was investigated in a mouse model. The calculated LD of BPG heated to cooking temperature (BPHCT) was 933.04 mg kg [b.w.] intraperitoneally corresponding to 3.75 times lower than its prototype. Mice fed on the experimental diet containing heat-treated beef with high BPG levels for 6 months displayed a reduction in body weight and altered serum values indicating for liver and renal function. Further, the organ ratios of intestinal and spleen were increased. Histopathological changes were observed in the liver, lung and parenchyma testis tissue. BPHCT residue induced sperm aberration and micronucleated polychromatic erythrocytes formation. Present results indicate that prolonged exposure of BPHCT at higher residue levels might have an impact on public health. Importantly the toxic concentrations of BPHCT are relatively high compared with levels that would result from the degradation of antibiotic residues in meat from animals that have received a therapeutic dose of BPG.

Cytotoxicity and degradation product identification of thermally treated ceftiofur

“…Previous studies in our laboratory also conrmed that penicillin, another b-lactam antibiotic, posed a public health risk aer cooking. 41,67 Considering the cytotoxicity of CEF-1, we should strengthen the detection of CEF or DFC in food.…”

Assessment of attractancy and safeness of (E)‐coniferyl alcohol for management of female adults of Oriental fruit fly, Bactrocera dorsalis (Hendel)

“…This study was performed under the auspices of the Institutional Animal Care and Use Committee at Henan University of Science and Technology, which consented to the experimental use of mice (Ethical Approval Number: 2021‐2105‐B006). The acute oral toxicity test was conducted according to the established methods 22,24,28,29 . In this experiment, ten female and ten male healthy‐looking mice were selected and divided randomly into two experimental groups.…”

“…The acute oral toxicity test was conducted according to the established methods. 22,24,28,29 In this experiment, ten female and ten male healthy-looking mice were selected and divided randomly into two experimental groups. Each group consisted of five males and five females.…”

Search for Biomarkers for the LC-ESI-QqQ Determination of Phenoxymethylpenicillin Treatment in Raw or Cooked Chicken Meat Samples

“…Moreover, the extensive illicit use of antibiotics may foster the appearance of antibiotic-resistant bacteria and constitutes a potential threat to human health . In this context, some studies have been devoted to the evaluation of the toxic effects of benzylpenicilloic acid or benzylpenicillin (PENG). , …”

“…3 In this context, some studies have been devoted to the evaluation of the toxic effects of benzylpenicilloic acid or benzylpenicillin (PENG). 4,5 To protect humans from uncontrolled exposure to any veterinary drugs, a withdrawal time has been imposed. The withdrawal time has been defined as the interval of time between the administration of a drug to the animal until its slaughter.…”