Acute and Sub-acute Oral Toxicity Study of Drepanoalpha® (A Poly-Herbal Formula Used in the Management of Sickle Cell Disease) in Guinea-pigs

Mpiana, Pius T.; Kasali, Félicien Mushagalusa; Bwirhonde, F.; Gbolo, Benjamin Z.; Tshibangu, Damien S. T.; Ngbolua, K. N.; Memvanga, Patrick B.; Kadima, Justin Ntokamunda

doi:10.9734/bjpr/2016/24200

Cited by 6 publications

(20 citation statements)

References 26 publications

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“…Indeed, the extract is toxic when it leads to a weight loss of about 10% or more (Schilter et al ., 2003, Chanda, 2015). These results corroborate with those obtained previously on lyophilisate and Drepanoalpha powder (Mpiana et al ., 2016; Ngbolua et al ., 2014).…”

Section: Discussionsupporting

confidence: 93%

“…However, this use of medicinal plants as medicines is simply based on traditional folklore use that has been perpetuated over several generations. With the increasing use of medicinal plants, a thorough scientific study of these plants is imperative, based on the need to validate their folkloric use (Mpiana et al ., 2016; Sofowora, 1989). Plants are supposed to be safe, but several dangerous and fatal side effects have been reported (Whitto et al ., 2003; Izzo, 2004).…”

Section: Introductionmentioning

confidence: 99%

“…Nevertheless, these products contain bioactive principles that may cause adverse effects (Bent and Ko, 2004). Thus, ensuring the safety, efficacy and quality of herbal products has become a key issue for the WHO (Mpiana et al ., 2016).…”

Section: Introductionmentioning

confidence: 99%

“…Pre-clinical studies have reported that Drepanoalpha is endowed with antifalcemic, antihemolytic, antiradical and antioxidant properties in vitro (Gbolo et al ., 2017b-c; Ngbolua et al ., 2014d). It has also shown the ability to increase red blood cell count, hemoglobin accumulation rate and platelets in rats, Guinea pigs and sickle cell patients (Gbolo et al ., 2017b-c; Ngbolua et al ., 2014d) and has no toxic effect on either rats or Guinea pigs (Mpiana et al ., 2016; Ngbolua et al ., 2014).…”

Section: Introductionmentioning

confidence: 99%

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In vivo Evaluation and in silico Prediction of the Toxicity of Drepanoalpha Hard capsules

Zoawe

Koto-Te-Nyiwa

Sha-Tshibey

et al. 2020

Preprint

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Drepanoalpha® hard capsules, a dry ethanolic extract (drug-extract ratio, 100/11) of a mixture of Justicia secunda Vahl and Moringa oleifera Lam dried leaves (1: 1, w/w) are used for the management of sickle cell disease in the Democratic Republic of Congo. Aim of the study: This phytomedicine safety was investigated by acute and sub-acute administration in Guinea pigs. Materials and methods: Healthy, male and nulliparous and non-pregnant female Guinea pigs were obtained from Laboratory of Animal Experimentation and Toxicology of the Department of Biology, Faculty of Sciences, University of Kinshasa. The animals were randomly selected, marked and divided into 2 groups of 5 animals each (3 males and 2 females) and 4 groups of 3 animals each for acute and sub-acute toxicity studies, respectively. The contents of hard capsules were dissolved in normal saline solution (NaCl 0.9 %). Animals received by gavage a single dose of 5000 mg/ kg of body weight (B.W.) of Drepanoalpha® hard capsules (acute toxicity) and 125 mg/ kg, 250 mg/ kg and 500 mg/ kg of B.W. twice daily for 28 days (sub-acute toxicity). Normal saline solution was used as control. Hematological, biochemical and histopathological analyses were performed and the behavior of the animals was observed after treatment. Results: The median lethal dose (LD50) is higher than 5000 mg/ kg of B.W., and the relative weights of vital organs (kidneys, liver and heart) collected from Guinea pigs at the end of treatment on D14 (acute toxicity) and D28 (sub-acute toxicity) has not undergone significant changes (p > 0.05). The results of haematological (red and white blood cells counts, haemoglobin, haematocrit) and biochemical (ALT, AST, albumin, total protein) tests did not show significant differences between control and test groups (α=0.05 for acute toxicity), while the histopathological study revealed no damage to the various organs excised. Conclusion: The results indicate the safety of Drepanoalpha® hard capsules, confirming previous studies, in rats and Guinea pigs, based on aqueous decoction of its raw herbs mixtures and the corresponding lyophilizate. Keywords: Acute toxicity, Sub-acute toxicity, Drepanoalpha® hard capsules

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Section: Discussionsupporting

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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In vivo Evaluation and in silico Prediction of the Toxicity of Drepanoalpha Hard capsules

Zoawe

Koto-Te-Nyiwa

Sha-Tshibey

et al. 2020

Preprint

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“…Serum creatinine is a good indicator of kidney function because any elevated serum is associated with failure of nephron function [34]. Therefore, our results showed that daily administration of Physalisperuviana is not affected in general, renal functions.…”

Section: Discussionmentioning

confidence: 54%

Evaluation of the Antidiabetic Properties of Hydro-Alcoholic Extract and Its Fractions from <i>Physalis peruviana</i> L. Leaves on Streptozotocin-Induced Diabetic Wistar Rats

Kasali¹

2016

JDMP

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The purpose of this study was to investigate the antidiabetic activity of extracts from leaves of Physalis peruviana L. used in the Eastern part of the Democratic Republic of the Congo used against diabetes. Different fractions with hexane, ethyl acetate and residue were obtained from the hydroalcoholic extract of Physalis peruviana leaves. The antidiabetic evaluation of hydroalcoholic and its fractions was evaluated in diabetic rats by a single administration of streptozotocin (50 mg/kg body weight) intravenously. The Reference group received glibenclamide (6.5 mg/kg body weight) and each test group received 100 mg/kg of body weight. Those groups were compared with a control group which received only a Tween 20 solution (1 ml per 100 g body weight). Serum biochemical profiles were evaluated by some blood markers including serum glucose, serum alanine transaminase (ALT), serum aspartate transaminase (AST) serum creatinine, total protein, triglyceride, total cholesterol, HDL-cholesterol and LDL-cholesterol. Antidiabetic profile evaluation showed no significant variation (P < 0.05) in blood glucose between groups after 28 days of treatment. There was no significant difference in the biochemical markers change including creatinine, ALT, AST, total protein, triglycerides, total cholesterol, HDL-cholesterol and LDL-cholesterol. The hydroalcoholic extract from the leaves of Physalis peruviana L. and its fractions showed antidiabetic activity suggesting future detailed studies for new chemical entities lead drug discovery.

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The Use of Natural Products in the Treatment of Sickle Cell Disease

de Paula,

Ribeiro,

de Melo Borges

et al. 2024

Rev. Bras. Farmacogn.

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Acute and Sub-acute Oral Toxicity Study of Drepanoalpha® (A Poly-Herbal Formula Used in the Management of Sickle Cell Disease) in Guinea-pigs

Cited by 6 publications

References 26 publications

In vivo Evaluation and in silico Prediction of the Toxicity of Drepanoalpha Hard capsules

In vivo Evaluation and in silico Prediction of the Toxicity of Drepanoalpha Hard capsules

Evaluation of the Antidiabetic Properties of Hydro-Alcoholic Extract and Its Fractions from <i>Physalis peruviana</i> L. Leaves on Streptozotocin-Induced Diabetic Wistar Rats

The Use of Natural Products in the Treatment of Sickle Cell Disease

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Acute and Sub-acute Oral Toxicity Study of Drepanoalpha® (A Poly-Herbal Formula Used in the Management of Sickle Cell Disease) in Guinea-pigs

Cited by 6 publications

References 26 publications

In vivo Evaluation and in silico Prediction of the Toxicity of Drepanoalpha Hard capsules

In vivo Evaluation and in silico Prediction of the Toxicity of Drepanoalpha Hard capsules

Evaluation of the Antidiabetic Properties of Hydro-Alcoholic Extract and Its Fractions from &lt;i&gt;Physalis peruviana&lt;/i&gt; L. Leaves on Streptozotocin-Induced Diabetic Wistar Rats

The Use of Natural Products in the Treatment of Sickle Cell Disease

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Product

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About

Evaluation of the Antidiabetic Properties of Hydro-Alcoholic Extract and Its Fractions from <i>Physalis peruviana</i> L. Leaves on Streptozotocin-Induced Diabetic Wistar Rats