Acute and sub-chronic toxicity of an aqueous extract of the leaves of Herniaria glabra in rodents

Rhiouani, Hamid; El-Hilaly, Jaouad; Israili, Zafar H.; Lyoussi, Badiaâ

doi:10.1016/j.jep.2008.05.009

Cited by 176 publications

(105 citation statements)

References 26 publications

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“…The present study recorded that AFP or AFG did not show any adverse effect on weight of these vital organs. Moreover, the present study assesses some liver function markers as liver is the chief target organ of acute toxicity (Rhiouania et al, 2008). The existing study revealed that AFP did not cause any functional change to the hepatocytes, as no obvious difference was observed between AFP and control rats.…”

Section: Discussionmentioning

confidence: 83%

Prospective Effect of Red Algae,actinotrichia Fragilis, Against Some Osteoarthritisaetiology

Sayed¹,

Sadek²,

Soliman³

et al. 2016

AJTCAM

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Background: Osteoarthritis (OA) is a progressive disease characterized by joints pain and articular cartilage destruction. Most of the current treatment strategies for OA are effective for symptoms relief but are accompanied with adverse side effect. Thus, the present investigation aims to evaluate the potential influence of red algae, Actinotrichia fragilis, in the dry powder form (AFP) or gel form (AFG) on some relevant factors of OA progression as well as assess its safety through in vitro and in vivo experiments. Materials and Methods: In vitro, AFP was analyzed for its chemical constituents screening, amino acid, proteinase inhibitory activity, HRBC membrane stabilization activity, free radical scavenging activity, total antioxidant potency, nitric oxide radical scavenging power. In vivo, Organization for Economic Co-operation and Development (OECD) toxicity test was performed to test the safety of AFP on rats.Results: The present findings revealed that AFP and AFG can be considered as inflammatory-proteinase-oxidant inhibitor and considered to be safe according to the OECD guideline. Conclusion: AFP and AFG may have the potency to become the therapeutic candidate for OA disease as it prevents the key causes of OA initiation.

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Section: Discussionmentioning

confidence: 83%

Prospective Effect of Red Algae,actinotrichia Fragilis, Against Some Osteoarthritisaetiology

Sayed¹,

Sadek²,

Soliman³

et al. 2016

AJTCAM

View full text Add to dashboard Cite

show abstract

“…As part of their defensive roles during inflammation, these cells release their lysosomal contents such as bactericidal enzymes and proteases exacerbating tissue damage (Chou, 1997;Okoli et al, 2008). Previous work done in inflammatory studies involving animal models had shown a clinical relevance in risk evaluation as the changes in hematological and immunological systems do have a higher predictive value when the data are translated from the animal studies to humans (Rhiouani et al, 2008). Further study showed that the CAHLF-treated groups had a significantly (p50.05) decreased total cholesterol, LDLcholesterol, VLDL-cholesterol, and triglycerides compared with the saline-treated control group induced arthritis for 21 d while HDL-cholesterol was found to be elevated in the treated groups.…”

Section: Discussionmentioning

confidence: 99%

Hematological and lipid profile evaluation of a hexane fraction ofCostus aferleaves in arthritic rats

Anyasor

Onajobi

Osilesi

et al. 2015

Pharmaceutical Biology

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Context: Costus afer Ker Gawl. (Costaceae) is an ethnomedical plant used as therapy against inflammatory disorders. Objective: The objective of this study was to evaluate the hematological and lipid profile analysis of hexane fraction of C. afer leaves (CAHLF) in arthritic rats. Materials and methods: Male albino rats were randomly distributed into seven groups of six rats each. Rats were induced with arthritis using formaldehyde and Complete Freund's Adjuvant (CFA) for 7 and 21 d, respectively. The animals were administered orally with 50, 100, and 250 mg/kg CAHLF, 10 mg/kg diclofenac and prednisolone, 0.9% NaCl (control), and 0.9% NaCl (normal). At the end of treatment periods, blood samples were withdrawn and subjected to hematological and biochemical analysis using auto-analyzer and spectrophotometric methods. Results: Hematological analysis revealed that in formaldehyde-and CFA-induced arthritic rat models, 250 mg/kg CAHLF-treated groups had significantly reduced (p50.05) hematocrit counts (HC) (30.98 ± 1.59% and 33.55 ± 1.10%), white blood cell counts ( /L). Furthermore, blood analysis showed that CAHLF-treated groups had reduced total cholesterol, low-density lipoprotein cholesterol, and triglycerides while they had an elevated high-density lipoprotein compared with the control group. Discussion and conclusion: Findings from this study indicated that CAHLF could possess immunomodulatory and hypolipidemic properties in arthritic rats. CAHLF could be considered as a source of biopharmaceutical agents in anti-arthritis drug discovery process.

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“…14 Body weight changes are indicators of adverse effects of chemical drugs and products, and it will be important if the loss of body weight is more than 10% from the initial weight. 15,16 However, it is reported that increases in body weights of the animals are more closely related to the accumulation of fat rather than toxic effects of drugs or chemicals 17 The reductions in body weights observed the first days of treatment may be associated with normal physiological responses of adaptation of animals to the extract 18 but also to stress caused by treatment. 19 Treatment of rats with ETDE at repeated doses for 28 days, caused no significant change (p<0.001) in hematological and biochemical parameters in rats treated compared to the control group.…”

Section: Discussionmentioning

confidence: 99%

Assessment of Acute and Subacute Toxicity of the Total Dichloromethane-Ethanol Extract of Morinda morindoides (Baker) Milne-Redh (ETDE) on Rats

Lucien¹,

Calixte²,

Gilles³

et al. 2015

Phcog J

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Context: ETDE shown good antihypertensive and antioxidant activities in rats made hypertensive. This present study aims to assess its toxicity. Aims: This study was designed to study the toxicity of dichloromethaneethanol extract of Morinda morindoides. Settings and Design: Toxicological activity in vivo. Methods and Material: Alkaloids were characterized from reagents of Bouchardat, flavonoids by reacting the cyanidrine, tannins by the reagent Stiasny, polyphenols by reacting ferric chloride, quinones by the reagent Bornstraëgen, sterols and polyterpenes by the reaction of Libermann and saponins by observing the foam after agitation of the extract. Acute and subacute toxicity were studied using respectively 423 and 407 OECD guidelines for testing of chemicals. Statistical analysis used:The graphical representation of the data was performed using the Graph Pad Prism 5.0. The mean value is accompanied by the standard error of the mean (Mean ± SEM). The difference between the two values is considered significant when P<0.001. Statistical analysis of results was performed using analysis of variance (ANOVA). Results: The phytochemical screening showed the presence in the ETDE of polyphenols, alkaloids, flavonoids, sterols and polyterpenes. The toxicological study shows that ETDE has a LD 50 between 2000 and 5000 mg/kg bw therefore classified in the hazard category 5. The administration of ETDE at repeated dose for 28 days did not significantly affect the weight gain, hematological and biochemical parameters of rats. Conclusion: ETDE toxicity is relatively low with LD 50 between 2000 and 5000 mg/kg bw. It does not cause damage to the heart, liver and kidney. ETDE can be used without risk of intoxication.

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Acute and sub-chronic toxicity of an aqueous extract of the leaves of Herniaria glabra in rodents

Cited by 176 publications

References 26 publications

Prospective Effect of Red Algae,actinotrichia Fragilis, Against Some Osteoarthritisaetiology

Prospective Effect of Red Algae,actinotrichia Fragilis, Against Some Osteoarthritisaetiology

Hematological and lipid profile evaluation of a hexane fraction ofCostus aferleaves in arthritic rats

Assessment of Acute and Subacute Toxicity of the Total Dichloromethane-Ethanol Extract of Morinda morindoides (Baker) Milne-Redh (ETDE) on Rats

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