Acute and Subacute Oral Toxicity Evaluation of Eriobotrya japonica Leaf Triterpene Acids in ICR Mice

Abstract

The interest focusing on Eriobotrya japonica leaf triterpene acid (ELTA) has increased recently because of its beneficial effects on health. However, there has been a lack of experimental data on its toxicity. The present study therefore was conducted to evaluate its toxicity in ICR mice. The results showed that ELTA produced neither mortality nor toxicity of the main organs in ICR male and female mice in both acute (0.30, 0.65, 1.39, and 3.00 g·kg−1 body weight) and subacute (150, 300, and 600 mg·kg−1 BW) 28-day toxicity studies. Because of lacking apparently adverse effects found in the hematology, clinical biochemistry, and histopathology evaluation, results of the present study together with the beneficial effects make ELTA as a promising dietary supplement and indicated that ELTA administered orally might have a large safety margin for human.

Targeted delivery of ursolic acid and oleanolic acid to lungs in the form of an inhaler for the management of tuberculosis: Pharmacokinetic and toxicity assessment

“…An acute toxicity study was performed here to understand the adverse effects of these drugs during the two weeks of inhalation exposure. Treatment of rats with OA and UA was found safe at the doses tested in an acute toxicity study [ 8 ]. In this study, no adverse effect of inhalation exposure was observed on rats.…”

“…This extract was tested for acute and subacute toxicity in ICR mice. No mortality or adverse clinical symptoms have been reported at 0.3 to 3.0 gm/kg body weight [ 8 ]. LD 50 > 300 mg/kg has been reported in mice with subcutaneous exposure to these drugs mixture obtained from Bouvardia ternifolia extract [ 9 ].…”

Vochysia tucanorum Mart. butanol fraction presents antitumoral activity in vivo and prevents the installation of cachexia in solid Ehrlich tumor model

“…Even though natural products may represent effective alternatives in the treatment of various diseases, it can also be a problem for health [ 30 ]. Ethnobotanical studies report the risks of intoxication and the side effects of those compounds [ 31 , 32 ]. Gomes et al (2009) demonstrated the absence of acute toxicity with single oral administration of 5000 mg/kg of V. tucanorum methanolic leaf extract.…”

Evaluation of Anti-Tumorigenic Effects of Diosmetin against Human Colon Cancer Xenografts in Athymic Nude Mice

“…Oral acute toxicity test was performed according to OECD guideline No 423 [25,26]. All animal experimental procedures were carried out according to the approval of the institutional Animal Care and Use Committee, Faculty of Medicine (FOM-IACUC), (Ethics reference No.…”

“…Absence or presence of compound-related mortality of the animals dosed at each step determined the following step of either dosing of three additional animals with the same dose or dosing of three additional animals at the next higher or the next lower dose levels. Since there is no information on the toxicity of Dis, 300 mg/kg body weight was used as the starting dose according to OECD 423 guideline [25,26]. In total, a maximum of 18 female ICR mice (4–6 weeks old, 20–24 g) were used in this experiment and the grouping was as follows: Group 1: normal control, which received distilled water orally, Group 2: 300 mg/kg Dis was administered as a single dose orally, Group 3: next higher dose level of Dis (2000 mg/kg) was administered as a single dose orally.…”