Acute and subacute toxicity evaluation of hydroalcoholic extract from the stem bark of Bois Bande (Parinari campestris Aubl.1772) in rats

Abstract

Introduction The bark of Bois Bande (Parinari campestris) is a popular aphrodisiac in the Caribbean that has been traditionally used for many years to restore sexual vitality, increase sperm count, and treat erectile dysfunction, without valid scientific data. Acute and 28-day subacute toxicity studies were conducted to evaluate the safety of the hydroalcoholic extract of P.campestris bark and to find a safe dose for human use in conventional medicine. Methods The acute toxicity study used a single oral dose of P.campestris extract at four separate doses, 5, 50, 300, and 2,000 mg/kg, and was seen for 14 days, while the subacute toxicity study used a daily oral dose of P.campestris extract at 3 different doses, 100, 300, and 1000 mg/kg/day for 28 days. Results The LD50 of P.campestris extract was found to be greater than 2000 mg/kg in the acute toxicity study. P.campestris extract did not show toxicity at 1000 mg/kg/day in subacute toxicity trial; NOAEL was 1000 mg/kg/day in rats. However, the body weight was increased in males. Conclusion In conclusion, 1000 mg/kg P.campestris extract can be considered safe and non-toxic in males.

Investigation and Biological Assessment of Rumex vesicarius L. Extract: Characterization of the Chemical Components and Antioxidant, Antimicrobial, Cytotoxic, and Anti-Dengue Vector Activity

“…The IC 50 of the R. vesicarius extract was estimated by plotting the sample absorbance against the log of doses at different concentrations of the serial dilution ( Figure 5 ). The response increased upon increasing the extract concentration, with the vertical point of the curve revealing the EC 50 value [ 69 , 70 ]. The data analysis showed that a dose of 500 µg/mL had a cytotoxic influence on HepG2 cell lines.…”

Bioactive Chemical Constituents of Matthiola longipetala Extract Showed Antioxidant, Antibacterial, and Cytotoxic Potency

“…The absorbance of the sample versus the log of extract dosages at various concentrations was plotted in order to determine the EC 50 value of the M. longipetala extract. While high concentrations of the plant extract produce a maximum reaction, low dosages of the extract are insufficient to elicit a response [61]; the vertical point of the curve revealed the EC 50 value. Regarding its cytotoxic impact on HepG2 cell lines, the extract of M. longipetala had an EC 50 value of 2.36 g/mL.…”