Acute and subchronic 90-days toxicity assessment of propyl-propane-thiosulfinate (PTS) in rats

Cascajosa-Lira, Antonio; Sánchez, Sílvia; Baños, Alberto; Guillamón, Enrique; Molina-Hernández, Verónica; Moyano, Rosario; Jos, Ángeles; Cameán, Ana M.

doi:10.1016/j.fct.2022.112827

Cited by 8 publications

(5 citation statements)

References 34 publications

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“…Further attention will also be important to determine the most effective means of reaching the levels of PTS-PTSO intake that are necessary to show biological effects. Whilst a moderately high level of garlic consumption in humans has been shown to result in PTS intake approaching 0.1 mg/kg body weight [ 42 ], this is still somewhat lower than the dosages used in our current experiments. Thus, it may be more feasible to use dietary supplementation with purified forms of PTS-PTSO, as is increasingly being performed, with some success, in livestock production [ 43 ].…”

Section: Discussionmentioning

confidence: 68%

Garlic-Derived Metabolites Exert Antioxidant Activity, Modulate Gut Microbiota Composition and Limit Citrobacter rodentium Infection in Mice

et al. 2022

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The garlic-derived compounds propyl propane thiosulfinate (PTS) and propyl propane thiosulfonate (PTSO) are metabolites with putative health benefits against intestinal inflammation that may be related to their antioxidant activity. However, the underlying mechanisms remain unclear, and whether PTS-PTSO can promote gut health by altering the microbiota and exert protection against enteric pathogens needs further investigation. Here, we explored the antioxidant activity of PTS-PTSO in murine macrophages in vitro, and in an in vivo model of bacterial infection with the bacterial pathogen Citrobacter rodentium. PTS-PTSO attenuated reactive oxygen species in lipopolysaccharide-stimulated macrophages in a nuclear factor erythroid factor 2-related factor 2 (Nrf2)-dependent manner, decreased nitric oxide levels both in macrophages in vitro and in the sera of mice fed PTS-PTSO, and had putatively beneficial effects on the commensal gut microbiota. Importantly, PTS-PTSO decreased faecal C. rodentium counts, concomitant with upregulation of Nrf2-related genes in colon tissue. Thus, PTS-PTSO mediates Nrf2-mediated antioxidant activity and modulates gut microbiota, which may protect the host against C. rodentium colonization. Our results provide further insight into how PTS-PTSO and related bioactive dietary compounds may reduce enteric infections.

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Section: Discussionmentioning

confidence: 68%

Garlic-Derived Metabolites Exert Antioxidant Activity, Modulate Gut Microbiota Composition and Limit Citrobacter rodentium Infection in Mice

et al. 2022

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“…In addition, the weight and blood pressure of the volunteers were followed every four weeks, and no significant changes were detected ( Figure 3 ). In fact, the safety of similar extracts [ 17 , 18 ] and compounds present in Alliaceae [ 19 , 20 , 21 ] has been previously analyzed in several studies, obtaining similar conclusions. In relation to weight, opposite effects were seen during the study.…”

Section: Discussionmentioning

confidence: 68%

Beneficial Effects of Daily Consumption of Garlic and Onion Extract Concentrate on Infectious Respiratory Diseases in Elderly Resident Volunteers

García-García

Gracián²,

Baños³

et al. 2023

Nutrients

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Aging is a biological process with high susceptibility to several infections. This risk increases in older patients in residential care facilities (RCF). Thus, there is a clear demand for developing preventive interventions with new therapeutic compounds that combine efficacy and safety. This could be the case of compounds derived from plants of the genus Allium spp. The purpose of this study was to evaluate the impact of a combination of a garlic and onion extract concentrate standardized in organosulfur compounds derived from propiin on the incidence of respiratory tract infections in elderly patients of RCF. Sixty-five volunteers were selected at random to receive a placebo or a single daily dose of the extract for thirty-six weeks. Different clinical visits were performed to evaluate the main respiratory diseases with an infectious origin, as well as the associated symptoms and their duration. The extract showed a clinical safety profile and significantly reduced the incidence of respiratory infections. Moreover, the treatment decreased the number and duration of the associated symptoms compared with the placebo group. For the first time, we demonstrated the protective effect of Alliaceae extract in respiratory infectious diseases in elderly healthy volunteers, which could be used prophylactically against the most common infectious respiratory diseases.

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“…Hematological and serum biochemical parameters are a comprehensive reflection of the degree of damage to an organism after systemic exposure to toxicants. Consistent with other toxicity assessment studies, we examined hematological parameters and serum enzymes that reflect liver and kidney function in mice [ 38 , 39 ]. In addition, cardiac enzymes and lipoprotein levels in serum were measured.…”

Section: Discussionmentioning

confidence: 99%

Toxicity assessment of Cucurbita pepo cv Dayangua and its effects on gut microbiota in mice

Zhang,

Zhou,

Pan

et al. 2024

BMC Complement Med Ther

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Background Cucurbita pepo cv Dayangua (CPD) is an edible plant with diverse pharmacological properties. The current research on CPD has primarily focused on initial investigations of its chemical composition and pharmacological effects, and no comprehensive toxicity assessment has been conducted to date. Methods In the present study, the toxicity of CPD was evaluated through both acute and sub-chronic oral toxicity tests in mice. 16S rDNA sequencing was used to analyze the composition of the gut microbiota of mice at different time points to observe the effect of CPD on these microbial communities. Results In the acute toxicity test, CPD exhibited low toxicity, with a median lethal dose (LD50) > 2000 mg/kg. The sub-chronic toxicity test indicated that CPD administration at doses of 200, 400, and 600 mg/kg did not cause mortality or significant organ damage in mice. Furthermore, analysis of the gut microbiota after gavage administration of CPD at 400 and 600 mg/kg revealed an improved abundance of some beneficial gut bacteria. Conclusions In summary, no acute or sub-chronic toxic effects were observed in mice following the oral administration of CPD. CPD did not affect the structure and diversity of the gut microbiota and may contribute to an increase in the number of beneficial gut bacteria.

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Acute and subchronic 90-days toxicity assessment of propyl-propane-thiosulfinate (PTS) in rats

Cited by 8 publications

References 34 publications

Garlic-Derived Metabolites Exert Antioxidant Activity, Modulate Gut Microbiota Composition and Limit Citrobacter rodentium Infection in Mice

Garlic-Derived Metabolites Exert Antioxidant Activity, Modulate Gut Microbiota Composition and Limit Citrobacter rodentium Infection in Mice

Beneficial Effects of Daily Consumption of Garlic and Onion Extract Concentrate on Infectious Respiratory Diseases in Elderly Resident Volunteers

Toxicity assessment of Cucurbita pepo cv Dayangua and its effects on gut microbiota in mice

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