Acute and subchronic toxicity of the antitumor agent rhodium (II) citrate in Balb/c mice after intraperitoneal administration

Carneiro, Marcella Lemos Brettas; Lopes, C.A.P.; Miranda-Vilela, Ana Luisa; Joanitti, Graziella Anselmo; Silva, Izabel C.R. da; Mortari, Márcia Renata; Souza, Aparecido Ribeiro de; Báo, Sônia Nair

doi:10.1016/j.toxrep.2015.07.010

Cited by 10 publications

(6 citation statements)

References 37 publications

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“…2A shows a micrograph of sample Mag-RhCit obtained by Transmission Electron Microscopy (TEM). It was observed that NPs are spherical and have polydispersity of a size expected for syntheses by co-precipitation route and as also observed by other authors [7,[21][22][23][24]28]…”

supporting

confidence: 76%

“…Previous studies with maghemite NPs and maghemite NPs functionalized with rhodium (II) citrate (Magh-RhCit) showed progress and great potential for chemotherapeutic treatment [7,[20][21][22][23][24]. However, as yet there are no studies related to the identification of the protein corona of this NP nor about its interaction in cells of the immune system.…”

Section: Introductionmentioning

confidence: 99%

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Formation of Protein Corona on Rhodium Citrate-Functionalized Magnetic Nanoparticles and Their Interaction with Human Macrophages

Lago¹,

Carvalho²,

Chaves³

et al. 2019

J Nanomed Nanotechnol

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Nanoscience and Nanotechnology enable innovations for Medicine and for Biomedical Science. Among the current nanoparticles, maghemite associated with rhodium citrate (Magh-RhCit) has been shown to be promising, because it reduces side effects of drugs while maintaining cytotoxicity for tumor cells. However, NPs in contact with biological fluids are immediately coated by proteins (protein corona) that are unique to each nanomaterial. In this study, the adsorption of the most abundant binding proteins was studied in vitro using a three-step analysis: (1) characterization of the magnetic fluid (Magh-RhCit) before and after incubation with serum; (2) identification and physical and biochemical analysis of protein corona; and (3) the cellular internalization of these nanoparticles in human macrophages. Magh-RhCit was initially obtained in the magnetite phase (Fe 3 O 4) via alkaline coprecipitation of Fe 2+ and Fe 3+ ions and subsequently oxidized to maghemite by the bubbling of oxygen gas in the suspension. Later, rhodium citrate was associated with nanoparticles. Dynamic Light Scattering data were used for characterization of the hydrodynamic diameter and zeta potential. The morphological characterization and measurement of the particles were obtained from Scanning and Transmission Electron Microscopy, and the X-ray Diffraction technique. The identification of the proteins was performed by Liquid Chromatograph coupled to Mass Spectrometry. Human blood serum altered the characteristics of the nanoparticle, making it less polydisperse, larger and with less negative zeta potential, indicating the formation of the protein corona. Forty-nine proteins (which mostly promote opsonization, phagocytosis and endocytosis in cells of the immune system) were identified and characterized: albumin, IgGs, apoliproteins, serpins, complement (C5), kinases, haptoglobin, glycoproteins and transferrin. Nanoparticle characterization and mass spectrometric data of the digested protein corona suggest improved biocompatibility. Moreover, results regarding nanoparticles' interaction with macrophages suggest that the corona may have profoun implications for in vitro and in vivo extrapolations and will require some consideration in the future.

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supporting

confidence: 76%

Section: Introductionmentioning

confidence: 99%

Formation of Protein Corona on Rhodium Citrate-Functionalized Magnetic Nanoparticles and Their Interaction with Human Macrophages

Lago¹,

Carvalho²,

Chaves³

et al. 2019

J Nanomed Nanotechnol

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“…Daily observation was performed for 14 days. The weight and intake conditions of the mice were recorded, as well as the potential toxic and death phenomena [10][11][12][13].…”

Section: Acute Toxicity Experiments In Micementioning

confidence: 99%

Comparison of the acute toxicity, analgesic and anti-inflammatory activities and chemical composition changes in Rhizoma anemones Raddeanae caused by vinegar processing

Wang

Zhou

Xie

et al. 2020

BMC Complement Med Ther

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Background: As the dry rhizome of Anemone raddeana Regel, Rhizoma Anemones Raddeanae (RAR), which belongs to Ranunculaceae, is usually used to treat wind and cold symptoms, hand-foot disease and spasms, joint pain and ulcer pain in China. It is well known that the efficacy of RAR can be distinctly enhanced by processing with vinegar due to the reduced toxicity and side effects. However, the entry of vinegar into liver channels can cause a series of problems. In this paper, the differences in the acute toxicity, anti-inflammatory and analgesic effects between RAR and vinegar-processed RAR were compared in detail. The changes in the chemical compositions between RAR and vinegar-processed RAR were investigated, and the mechanism of vinegar processing was also explored. Methods: Acute toxicity experiments were used to examine the toxicity of vinegar-processed RAR. A series of studies, such as the writhing reaction, ear swelling experiment, complete Freund's adjuvant-induced rat foot swelling experiment and cotton granuloma, in experimental mice was conducted to observe the anti-inflammatory effect of vinegar-processed RAR. The inflammatory cytokines of model rats were determined by enzyme-linked immunosorbent assay (ELISA). Liquid Chromatography-Quadrupole-Time of Flight mass spectrometer Detector (LC-Q-TOF) was used to analyse the chemical compositions of the RARs before and after vinegar processing. Results: Neither obvious changes in mice nor death phenomena were observed as the amount of vinegar-processed RAR in crude drug was set at 2.1 g/kg. Vinegar-processed RAR could significantly prolong the latency, reduce the writhing reaction time to reduce the severity of ear swelling and foot swelling, and remarkably inhibit the secretion of Interleukin-1β(IL-1β), Interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) proinflammatory cytokines. The content of twelve saponins (e.g., Eleutheroside K) in RAR was decreased after vinegar processing, but six other types (e.g., RDA) were increased. Conclusions: These results revealed that vinegar processing could not only improve the analgesic and antiinflammatory effects of RAR but also reduce its own toxicity. Trial registration: Not applicable.

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“…The use of drugs in the treatment of thyroid cancer should be analyzed by efficiency in the healing process and side effects. In general, adverse effects related to drug toxicity depend on parameters, such as dose, routine, and period of medication administration (Carneiro et al 2015). In Traino and Di Martino (2006), the authors discuss from some mathematical models the quantities of 131 I for the ablation of thyroid remnants postsurgery or metastasis.…”

Section: Resultsmentioning

confidence: 99%

Mathematical models applied to thyroid cancer

et al. 2019

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Thyroid cancer is the most prevalent endocrine neoplasia in the world. The use of mathematical models on the development of tumors has yielded numerous results in this field and modeling with differential equations is present in many papers on cancer. In order to know the use of mathematical models with differential equations or similar in the study of thyroid cancer, studies since 2006 to date was reviewed. Systems with ordinary or partial differential equations were the means most frequently adopted by the authors. The models deal with tumor growth, effective half-life of radioiodine applied after thyroidectomy, the treatment with iodine-131, thyroid volume before thyroidectomy, and others. The variables usually employed in the models includes tumor volume, thyroid volume, amount of iodine, thyroglobulin and thyroxine hormone, radioiodine activity, and physical characteristics such as pressure, density, and displacement of the thyroid molecules. In conclusion, the mathematical models used so far with differential equations approach several aspects of thyroid cancer, including participation in methods of execution or follow-up of treatments. With the development of new models, an increase in the current understanding of the detection, evolution, and treatment of diseases is a step that should be considered.

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Acute and subchronic toxicity of the antitumor agent rhodium (II) citrate in Balb/c mice after intraperitoneal administration

Cited by 10 publications

References 37 publications

Formation of Protein Corona on Rhodium Citrate-Functionalized Magnetic Nanoparticles and Their Interaction with Human Macrophages

Formation of Protein Corona on Rhodium Citrate-Functionalized Magnetic Nanoparticles and Their Interaction with Human Macrophages

Comparison of the acute toxicity, analgesic and anti-inflammatory activities and chemical composition changes in Rhizoma anemones Raddeanae caused by vinegar processing

Mathematical models applied to thyroid cancer

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