Acute Effect of High‐dose Isoflavones from Pueraria lobata (Willd.) Ohwi on Lipid and Bone Metabolism in Ovariectomized Mice

Abstract

We investigated the acute metabolic effects of isoflavones from Pueraria lobata (Willd.) Ohwi (IPL) in ovariectomized (OVX) mice. After 4 weeks of IPL feeding at 500 mg/day/kg body weight (OVX500), plasma 17β-estradiol concentrations were significantly higher (+25%, p < 0.05), whereas plasma triglyceride levels were significantly lower in OVX mice (-15%, p < 0.05) compared with controls. Abdominal adipose tissue weight was marginally reduced in IPL-fed groups compared with OVX controls and the plasma levels of liver enzymes were unchanged. In addition, IPL significantly inhibited the reduction of bone mineral density in the femurs of OVX mice (OVX200, +22%; OVX500, +26%; p < 0.05) compared with controls after 4 weeks of IPL feeding. In quantitative polymerase chain reaction analysis the expression of aromatase was significantly suppressed and SULT1E1 was increased by IPL feeding, showing that IPL feeding may not alter the risk for breast cancer in mice. Our results suggest that IPL could ameliorate menopausal symptoms in mice. Further studies will confirm the effects of IPL in humans.

Evidence for estrogeno-mimetic effects of a mixture of low-dose pollutants in a model of ovariectomized mice

“…However, EST mRNA levels were reduced to their nadir after ovariectomy with no change in the pollutant-exposed mice along with circulating estrogen levels reduced to low levels (data not shown). These findings are consistent with the literature indicating that EST gene expression depends on estrogen levels and that its involvement in the regulation of estrogen bioavaibility is lost in ovariectomized animals (Cho et al, 2012).…”

Development of a colloidal gold nanoparticle‐based immunochromatographic strip for the one‐step detection of miroestrol and puerarin

“…From previously reports, the average amount of miroestrol in P. candollei was 8–27 μg/g dry weight (Yusakul et al, ; Yusakul, Putalun, Udomsin, Juengwatanatrakul, & Chaichantipyuth, ), while the concentration of puerarin was found to vary among plant species, e.g. P. candollei, 120–4,000 μg/g dry weight (Cherdshewasart et al, ; Cherdshewasart & Sriwatcharakul, ; Manosroi & Manosroi, ; Pongkitwitoon et al, ) and P. lobata , 75–13,000 μg/g dry weight (Cho et al, ; Udomsin et al, ). Therefore, when compared with previous reports, our developed method is the optimal sensitivity for the detection of both miroestrol and puerarin in the samples.…”

Novel Insights into the Pharmacology of Flavonoids

“…A number of recent reports have shown that isoflavones can ameliorate experimental menopausal symptoms. For example, Cho and Jun reported that isoflavones from Pueraria lobata inhibited the reduction of bone mineral density in the femurs of ovariectomized mice (Cho et al ., ). Another study showed that two isoflavones, i.e.…”