Acute effect of phosphodiesterase type 5 inhibitor on serum oxidative status and prolidase activities in men with erectile dysfunction

Savaş, Murat; Yeni, Ercan; Verit, Ayhan; Gülüm, Mehmet; Aksoy, Nurten; Çiftçi, Halil; Çelik, Hakim; Altunkol, Adem; Öncel, Halil Ferat

doi:10.1590/s1807-59322010001200014

Cited by 16 publications

(15 citation statements)

References 26 publications

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“…Ozgur et al 68 reported that udenafil decreased the MDA level in ischemia-reperfusion injury caused by rat testicular torsion/detorsion. Savas¸et al 69 reported that tadalafil decreased the total oxidant status (TOS) and increased the total antioxidant status (TAS) in men with erectile dysfunction. In the present study, we determined the MDA levels in our groups, and our results were similar to previous studies.…”

Section: Discussionmentioning

confidence: 99%

The effect of PDE5 inhibitors on bone and oxidative damage in ovariectomy-induced osteoporosis

Hakan

Huyut

Yıldırım

et al. 2017

Exp Biol Med (Maywood)

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The results in our study appear to establish the osteoporosis model and provide evidence of the positive effects of three separate PDE5 inhibitors (vardenafil, udenafil, and tadalafil). The positive effects of these PDE5 inhibitors are investigated and demonstrated by the bone mass density and bone resorption markers. These effects are associated with significant demonstrated antioxidant activities. Osteoporosis is a significant major public health problem especially in more aged populations. Advances in identifying and understanding new potential therapeutic modalities for this disease are significant. This study provides such an advance. AbstractOsteoporosis is a major public health problem associated with many factors, and it affects more than 50% of women over 50 years old. In the current study, our purpose was to investigate the effects of phosphodiestarase-5 inhibitors on osteoporosis via the nitric oxide/3 0 ,5 0 -cyclic guanosine monophosphate/protein kinase G signalling pathway. A total of 50 female albino Wistar rats were separated into five groups. The first group was appointed as the healthy control group with no ovariectomy. All animals in the other groups underwent a bilateral ovariectomy. Six months after the ovariectomy, vardenafil, udenafil and tadalafil were given to the third, fourth and fifth groups, respectively, but were not administered to the positive control group (10 mg/kg per day for two months). The bone mineral density values were determined using a densitometry apparatus for all groups pre-and post-ovariectomy as well as after treatment. The levels of nitric oxide, endothelial nitric oxidesynthase, asymmetric dimethylarginine, 3 0 ,5 0 -cyclic guanosine monophosphate, protein kinase G, phosphodiestarase-5, pyridinoline, deoxypyridinoline, carboxyterminal telopeptide fragments and plasma carboxy terminal propeptide of type I collagen were determined using an enzyme linked immunosorbent assay. The levels of malondialdehyde, 8-hydroxy-2-deoxy guanosine, deoxyguanosine and coenzyme Q10 were determined by a high-performance liquid chromatography assay. Additionally, the right femoral trabecular bone density and the epiphyseal plate were measured in all groups. Angiogenesis was histologically observed in the bone tissue. In addition, we determined that the inhibitors may have caused a positive impact on the increased bone mass density and reduction of bone resorption markers. We also observed the positive effects of these inhibitors on oxidative stress. In conclusion, these phosphodiestarase-5 inhibitors increase angiogenesis in bone tissue and improve the re-formation rate of bone in rats with osteoporosis.

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Section: Discussionmentioning

confidence: 99%

The effect of PDE5 inhibitors on bone and oxidative damage in ovariectomy-induced osteoporosis

Hakan

Huyut

Yıldırım

et al. 2017

Exp Biol Med (Maywood)

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“…Prolidase activity has been shown in plasma erythrocytes, leucocytes, and fibroblasts and in organs like the kidney, the brain, the heart, the liver, the small intestines, the stomach, the lung, the spleen, the thymus, and the uterus [19]. While a higher prolidase activity is observed in patients with osteoporosis, osteoarthritis, hypertension, coronary artery disease, and erectile dysfunction [8, 9, 17, 18, 20, 21], a lower level is observed in renal insufficiency because the main source of prolidase is the kidney [19]. The severity of the antioxidant stress is directly correlated with the inhibition of collagen production in which prolidase is the main enzyme [19].…”

Section: Discussionmentioning

confidence: 99%

“…Increased levels of prolidase seem to be associated with increased levels of nitric oxide and oxidative stress along with decreased levels of antioxidant [7]. Also studies on vasculogenic erectile dysfunction have detected higher serum levels of prolidase [8, 9]. In this study we aimed to examine the effect of hCG on prolidase activity, oxidative stress, and levels of antioxidant enzyme in the testicles and penile tissue in a rat model.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Prolidase Activity, Oxidative Stress, and Antioxidant Enzyme Levels in Testicular and Penile Tissues after Human Chorionic Gonadotropin Treatment in Rats by Predicting Infertility and Erectile Dysfunction

et al. 2018

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Objectives: Prolidase plays a vital role in collagen turnover, matrix remodeling, and cell growth. We aimed to evaluate the association between treatment with chorionic gonadotropin and infertility and erectile dysfunction by investigating tissue prolidase activity, oxidative stress, and levels of antioxidant enzymes. Materials and Methods: The 16 male Wistar albino rats used in this study were randomly divided into 2 groups: rats treated with human chorionic gonadotropin (hCG) and control rats (n = 8 in each group). The rats in the hCG group were subcutaneously injected with 50 IU hCG daily for 15 days, while the rats in the control group were subcutaneously injected isotonic saline. All of the rats were sacrificed by a lethal overdose of sodium pentobarbital at the first month after hCG administration. Prolidase activity and levels of malonyl aldehyde, glutathione reductase, superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and catalase (CAT) were estimated in the testicular and penile tissue. The testicles and penis were transversely dissected and placed in formalin. Results: Levels of prolidase and malonyl aldehyde in the testicular and penile tissues were significantly higher in the hCG group than in the control group (p < 0.001), while levels of glutathione reductase, SOD, GSH-Px, and CAT were significantly lower in the hCG group than in the control group (p < 0.001). Conclusions: In this study, we observed that treatment with hCG increased prolidase activity and oxidative stress and decreased the antioxidant capacity of penile and testicular tissues; therefore, this may affect fertility and erectile function.

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“…Hücrede cGMP (siklik guanozin monofosfat) yıkımını engelleyen ve stoplazmik cGMP konsantrasyonunu arttırarak etkisini göste-ren bu grup ilaçların anti-oksidan etkilerini gösteren çalışmalar da vardır (14)(15)(16)(17)(18)(19)(20)(21)(22). Farklı dozlarda ve farklı sürelerde uygulanan PDE5 inhibitörlerinin; diyabet (14,15), iskemi-reperfüzyon (16)(17)(18), pulmoner hipertansiyon (19,20) ve kardiyomiyopati (21) gibi deneysel modellerde kalp, böbrek, over ve kavernöz dokularda oksidatif stresi azaltıp fonksiyonel iyileşme sağladığı gösterilmiştir.…”

Section: Introductionunclassified

“…Farklı dozlarda ve farklı sürelerde uygulanan PDE5 inhibitörlerinin; diyabet (14,15), iskemi-reperfüzyon (16)(17)(18), pulmoner hipertansiyon (19,20) ve kardiyomiyopati (21) gibi deneysel modellerde kalp, böbrek, over ve kavernöz dokularda oksidatif stresi azaltıp fonksiyonel iyileşme sağladığı gösterilmiştir. Bunun yanı sıra erektil disfonksiyonlu hastalarda PDE5 inhibitör-lerinin akut olarak serum oksidatif stres düzeyini azalttığı bildirilmiştir (22).…”

Section: Introductionunclassified

Oxidative Stress Induced by Ureteral Obstruction and Tadalafil

Cırrık¹,

Ayyıldız²,

Benli̇³

et al. 2017

JAREM

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Acute effect of phosphodiesterase type 5 inhibitor on serum oxidative status and prolidase activities in men with erectile dysfunction

Cited by 16 publications

References 26 publications

The effect of PDE5 inhibitors on bone and oxidative damage in ovariectomy-induced osteoporosis

The effect of PDE5 inhibitors on bone and oxidative damage in ovariectomy-induced osteoporosis

Evaluation of Prolidase Activity, Oxidative Stress, and Antioxidant Enzyme Levels in Testicular and Penile Tissues after Human Chorionic Gonadotropin Treatment in Rats by Predicting Infertility and Erectile Dysfunction

Oxidative Stress Induced by Ureteral Obstruction and Tadalafil

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