Acute Effects of Dihydrocapsaicin and Capsaicin on the Distribution of White Blood Cells in Rats

Abstract

SummaryThe acute effects of dihydrocapsaicin (DHC) and capsaicin (CAP) on the number of white blood cells (WBCs), neutrophils, eosinophils, basophils, monocytes, lymphocytes, T lymphocytes, B lymphocytes and NK cells, and serum corticosterone levels were studied in rats. Male 7-wk-old SD rats were divided into DHC (3.0 mg/kg BW), CAP (3.0 mg/ kg BW) and control (CON) groups. The number of total WBCs was 1.30-1.42 times significantly higher in the DHC group than in the CON group at 6-12 h. The number of neutrophils was 1.62 times significantly higher in the DHC group than in the CON group at 12 h. The number of total WBCs and neutrophils, however, showed no significant changes between the CAP and CON groups. The number of lymphocytes was 0.61 and 0.70 times significantly lower in the DHC and CAP groups than in the CON group at 3 h. The number of T lymphocytes and B lymphocytes was 0.74 and 0.54 times lower in the DHC group than in the CON group, respectively. CAP, however, did not significantly change the number of T lymphocytes or B lymphocytes. No significant changes in the number of NK cells were observed among the three groups. CAP and DHC did not change the number of monocytes, eosinophils or basophils. No significant changes of the serum corticosterone levels were observed among the three groups. In conclusion, capsaicinoids decreased the number of acquired immunity cells, and increased the number of total WBCs and neutrophils without changing the number of monocytes, eosinophils or basophils. The magnitidue of these effects was relatively higher in DHC than in CAP.

Acute and Subacute Effects of Dexamethasone on the Number of White Blood Cells in Rats

“…The combination of lymphocytes, neutrophil and monocyte together accounts for more than 98% of the number of total peripheral WBCs in rats. 25) In the present study, dexamethasone administration markedly decreased the number of WBCs, lymphocytes and monocyte at 1-3 hr after injection ( Fig. 2A-2C).…”

“…1B). 5,[23][24][25][26] Statistical Analyses --Experimental data were presented as mean ± SEM. The effects of dexamethasone on the number of WBCs were evaluated by a two-way analysis of variance (ANOVA) for repeated measures.…”

“…1A). 5,17,[22][23][24][25] Plasma immunoglobulin G (IgG) concentrations were assayed by our routine method of enzyme-linked immunosorbent assay (ELISA) until 24 hr after dexamethasone administration. Subacute Effects of Dexamethasone on the Number of WBCs --After 5 days of prefeeding, the rats were divided into dexamethasone (dose = 1.0 mg/kg BW per day, n = 11, the initial BW = 259 ± 3 g, mean ± SEM) and control groups (n = 10, the initial BW = 260 ± 2 g, mean ± SEM).…”

Diallyl Disulfide Reduced Dose-Dependently the Number of Lymphocyte Subsets and Monocytes in Rats

“…Lighting was automatically provided from 8:30-20:30. Animal chow (CE-2 cubic type, CLEA Japan, Inc.) and distilled water were given to the rats ad libitum (17). After the adaptation period, the rats were randomly divided into the DADS-free and DADS-orally administered (doseϭ10, 20, and 40 mg/kg BW) groups (nϭ8-9/group).…”

Subacute Effects of Capsaicinoids on the Distribution of White Blood Cells in Rats

“…1) Recently, we reported that a single administration [dose = 3 mg/kg body weight (BW)] of capsaicinoids such as DHC and CAP to rats decreased significantly the number of total lymphocytes, T-lymphocyte and Blymphocyte without changing the number of natural killer (NK) cells. 8) However, the subacute and chronic effects of capsaicinoids on the number and distribution of white blood cells (WBCs) are still unknown. In the present study, we studied the subacute effects of DHC and CAP on the number of total WBCs, lymphocytes, monocyte, neutrophil, eosinophil and basophil in rats.…”