Acute Effects of Ethanol on Hippocampal Spatial Representation and Offline Reactivation

Miyake, Kosaku; Yagi, Saichiro; Aoki, Yuki; Shikano, Yu; Ikegaya, Yuji; Sasaki, Takuya

doi:10.3389/fncel.2020.571175

Cited by 7 publications

(7 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It is necessary for acquiring spatial information to have an intact hippocampus [ 20 ]. HE staining showed that compared with the control group, the model group showed the disordered arrangement of hippocampal neurons and infiltration of inflammatory cells.…”

Section: Resultsmentioning

confidence: 99%

Perillaldehyde improves cognitive function in vivo and in vitro by inhibiting neuronal damage via blocking TRPM2/NMDAR pathway

et al. 2021

View full text Add to dashboard Cite

Background Vascular cognitive dysfunction in patients with vascular dementia (VD) is a kind of severe cognitive dysfunction syndrome caused by cerebrovascular diseases. At present, effective drugs to improve the cognitive function of VD patients still need to be explored. Transient Receptor Potential Melastatin 2 (TRPM2) channel is a nonspecific cation channel that plays a key role in the toxic death of neurons. Perillaldehyde (PAE) has the protective effect of epilepsy and insomnia and other central nervous system diseases. The aim of this study is to explore whether PAE improves cognitive function in VD rats and to investigate the potential mechanisms in vivo and vitro. Methods VD rats were induced by bilateral common carotid arteries occlusion (2-vessel occlusion [2VO]) and treated with PAE for 4 weeks. The neuroprotective effects of PAE was subsequently assessed by the Morris water maze, hematoxylin–eosin (HE) staining, Golgi staining, electron microscopy, Neuron-specific nuclear protein (Neu N) staining, and TdT-mediated dUTP nick end labeling (TUNEL) staining. After primary hippocampal neurons were isolated, cell viability was detected by MTT assay and intracellular Ca2+ concentration was detected by calcium imaging assay. The content of Nitriteoxide (NO), Malondialdehyde (MDA) and Superoxide dismutase (SOD) activity in serum of rats were observed by Enzyme Linked Immunosorbent Assay (ELISA). Immunohistochemistry, Western blot, and Confocal laser scanning were used to detect the expression levels of N-methyl-d-asprtate receptor-2B (NR2B) and TRPM2. Results The results showed that PAE can improve the number and activity of neurons, increase the length and number of dendrites in hippocampus, decrease the Vv value and PE value of neuronal nucleus and mitochondrial structure significantly, increase the s value and L value in nucleus structure, decrease the s value and L value in mitochondrial structure, and improve the learning and memory ability of rats significantly. And PAE can strengthen the ability of antioxidant stress confirmed by increasing the activity of SOD and reducing the production of MDA. The results of western blot, immunohistochemistry and immunofluorescence showed that PAE could reduce the level of TRPM2 and increase the expression of NR2B. Conclusions Taken together, our findings provide evidence that the neuroprotective effects of PAE in VD rats maybe through TRPM2 inhibition and subsequent activation of NMDAR signaling pathway.

show abstract

Section: Resultsmentioning

confidence: 99%

Perillaldehyde improves cognitive function in vivo and in vitro by inhibiting neuronal damage via blocking TRPM2/NMDAR pathway

et al. 2021

View full text Add to dashboard Cite

show abstract

“…In a prior experiment conducted by Miyake et al, the effect of acute EtOH administration on the spike patterns of hippocampal cell populations was analysed. The results from their study suggested that EtOH does not significantly alter the SWRs frequency in the hippocampal region (Miyake et al, 2020). While these studies provide valuable insights into the effects of EtOH exposure on SWRs waveforms, further investigations are warranted to fully elucidate the complex relationship between chronic alcohol use and SWRs.…”

Section: Role Of Ripples On Memory Consolidationmentioning

confidence: 99%

“…To the best of the author's knowledge, the evaluation of SWRs in association with memory impairment induced by alcohol administration has been limited to a small number of articles. Specifically, one study examined the response to acute alcohol administration in adult rats (Miyake et al, 2020), while another study investigated the effects of prenatal alcohol exposure on SWR activity (Harvey et al, 2021). The study of the effect of alcohol on SWRs as a memory consolidation biomarker may provide an opportunity for new forms of management to restore and improve cognitive impairment in alcoholism.…”

Section: Perspectivesmentioning

confidence: 99%

“…They indicate the reactivation of neural sequences through the simultaneous presence of two connected yet distinct events: a pronounced deflection in the local field potential called sharp wave and a rapid oscillation referred to as ripple (Buzsáki et al, 1992; Ylinen et al, 1995). In addition, the effect of alcohol on memory has been studied previously (Abrahao et al, 2017; Hamel et al, 2022; Miyake et al, 2020); however, to the authors' knowledge, SWRs have not been evaluated in association with memory impairment by chronic alcohol administration.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

A scoping review of the relationship between alcohol, memory consolidation and ripple activity: An overview of common methodologies to analyse ripples

Ruelas,

Medina‐Ceja,

Fuentes‐Aguilar

2023

Eur J of Neuroscience

View full text Add to dashboard Cite

Alcohol abuse is not only responsible for 5.3% of the total deaths in the world but also has a substantial impact on neurological and memory disabilities throughout the population. One extensively studied brain area involved in cognitive functions is the hippocampus. Evidence in several rodent models has shown that ethanol produces cognitive impairment in hippocampal‐dependent tasks and that the damage is varied according to the stage of development at which the rodent was exposed to ethanol and the dose. To the authors' knowledge, there is a biomarker for cognitive processes in the hippocampus that remains relatively understudied in association with memory impairment by alcohol administration. This biomarker is called sharp wave–ripples (SWRs) which are synchronous neuronal population events that are well known to be involved in memory consolidation. Methodologies for facilitated or automatic identification of ripples and their analysis have been reported for a wider bandwidth than SWRs. This review is focused on communicating the state of the art about the relationship between alcohol, memory consolidation and ripple activity, as well as the use of the common methodologies to identify SWRs automatically.

show abstract

“…The effects of acute ethanol (EtOH) on learning and memory have been investigated in human and rodent students, and memory deficits are usually reported (Lister et al , 1987; Silvers et al , 2003; Matthews and Silvers, 2004; Zorumski et al , 2014; Miyake et al , 2020). Nevertheless, these effects are dependent on several factors such as behavioral task, dose, acquisition/retrieval interval, age, sex, and estrogen levels (Matthews et al, 1995; 1999; Kameda et al , 2007; Broadwater and Spear, 2013; Boissoneault et al , 2014; Hoffman et al , 2015; Hunt and Barnet, 2016; Lewis et al , 2019; Sircar, 2019).…”

Section: Introductionmentioning

confidence: 99%

Sex differences in the acute ethanol effects on object recognition memory: influence of estrous cycle

Paiva-Santos

Leão

Kurita

et al. 2022

Behavioural Pharmacology

View full text Add to dashboard Cite

Effects of acute ethanol (EtOH) on memory depend on several factors, including type of behavioral task. Sex differences in EtOH effects have been reported in humans and animals, and recognition memory can be influenced by circulating sex hormones. The aim of this study was to investigate the influence of sex and estrous cycle in the acute effects of EtOH on novel object recognition memory in rats. Male and female Wistar rats were part of one of the groups: control, 0.6-g/kg EtOH and 1.8-g/kg EtOH (administered intraperitoneally before the training session). The estrous cycle was evaluated by vaginal smear. The task was conducted in an open field arena.During training, animals were exposed to two identical objects, and test sessions were performed 1 h (short-term) and 24 h (long-term) later. One of the objects was changed in each test. Increased novel object exploration was shown by male and female controls in the short-and long-term tests, respectively. In the short-term test, females did not show preference for the novel object, and EtOH 1.8 g/ kg impaired performance in males. In the long-term test, both sexes showed object discrimination, and 1.8-g/kg EtOH reduced preference for the new object in male rats. The phase of the cycle, the performance on proestrus was worse compared with other phases, and EtOH failed to impair performance mainly on estrous. In conclusion, while male rats displayed ethanol-induced recognition memory deficit, female rats were unaffected by EtOH impairing effects. In addition, the performance of female rats was influenced by the estrous cycle phases. Behavioural

show abstract

Acute Effects of Ethanol on Hippocampal Spatial Representation and Offline Reactivation

Cited by 7 publications

References 36 publications

Perillaldehyde improves cognitive function in vivo and in vitro by inhibiting neuronal damage via blocking TRPM2/NMDAR pathway

Perillaldehyde improves cognitive function in vivo and in vitro by inhibiting neuronal damage via blocking TRPM2/NMDAR pathway

A scoping review of the relationship between alcohol, memory consolidation and ripple activity: An overview of common methodologies to analyse ripples

Sex differences in the acute ethanol effects on object recognition memory: influence of estrous cycle

Contact Info

Product

Resources

About