Acute Ethanol Exposure Augments Low-Dose UVB-Mediated Systemic Immunosuppression via Enhanced Production of Platelet-Activating Factor Receptor Agonists

Travers, Jeffrey B.; Weyerbacher, Jonathan; Ocaña, Jesus A.; Borchers, Christina E.; Rapp, Christine M.; Sahu, Ravi P.

doi:10.1016/j.jid.2018.11.034

Cited by 8 publications

(6 citation statements)

References 16 publications

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“…UVB source was a Philips F20T12/UVB lamp. The intensity of the UVB source was measured before each experiment using an IL1700 radiometer and a SED240 UVB detector (International Light) (30). The mice were then returned to their home cages.…”

Section: Methodsmentioning

confidence: 99%

“…All mice were randomly divided into three groups ( n = 4/group): non‐UVB, low‐UVB fluence (2500 J m −2 ), high‐UVB fluence (7500 J m −2 ). UVB irradiation was applied for 0, 13 min 45 s and 41 min 15 s on the shaved dorsal skin, corresponding to the low‐UVB (2500 J m −2 ) (30) and high‐UVB (7500 J m −2 ) (18). UVB source was a Philips F20T12/UVB lamp.…”

Section: Methodsmentioning

confidence: 99%

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Ultraviolet B Irradiation Alters the Level and miR Contents of Exosomes Released by Keratinocytes in Diabetic Condition

Wang

Pothana

Chen

et al. 2022

Photochem & Photobiology

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Ultraviolet B (UVB) stimulates the generation of extracellular vesicles, which elicit systemic effects. Here, we studied whether UVB affects the release and microRNA (miR) content of keratinocyte exosomes (EXs) in diabetic conditions. In vitro, we examined the UVB effects on affecting EX release from keratinocyte HaCaT cells (HaCaT-EX) pretreated with high glucose. HaCaT-EX functions were evaluated on Schwann cells (SCs). In vivo, UVB-induced miR change in skin EXs of diabetic db/db mice was analyzed. The miRs of interest were validated in HaCaT-EXs. We found that: (1) UVB promoted HaCaT-EX generation in dose-and timedependent manners; 100 and 1800 J m −2 of UVB had the most prominent effect and were selected as effective low-and high-fluence UVB in vitro. (2) A total of 13 miRs were differentially expressed >3-fold in skin EXs in UVB-treated db/db mice; miR-126 was the most up-regulated by low-fluence UVB. (3) Functional studies revealed that the SC viability was improved by low-fluence UVB HaCaT-EXs, while worsened by high-fluence UVB HaCaT-EXs. (4) MiR-126 inhibitor attenuated the effects induced by low-fluence UVB HaCaT-EXs. Our data have demonstrated that low-and high-fluence UVBs promote HaCaT-EX generation but differentially affect exosomal miR levels and functions under diabetic conditions.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Ultraviolet B Irradiation Alters the Level and miR Contents of Exosomes Released by Keratinocytes in Diabetic Condition

Wang

Pothana

Chen

et al. 2022

Photochem & Photobiology

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“…Combination of ethanol exposure and low dose UVB radiation resulted in increased PAFR agonist production 39 . Similarly, combination of ethanol with TBI likewise enhanced PAF and proinflammatory cytokine production 15,43 . Ethanol exposure increased PAFR agonist activity when combined with UVB radiation in HaCaT cell culture, human explants, and murine models 43 .…”

Section: Mvps and Itbimentioning

confidence: 96%

“…39 Similarly, combination of ethanol with TBI likewise enhanced PAF and proinflammatory cytokine production. 15,43 Ethanol exposure increased PAFR agonist activity when combined with UVB radiation in HaCaT cell culture, human explants, and murine models. 43 Additionally, immunosuppression has been found to be exacerbated by ethanol and TBI combination injury, where T-cell and dendrite cell counts were decreased by the ethanol exposure in rats.…”

Section: Mvps and Itbimentioning

confidence: 99%

“…15,43 Ethanol exposure increased PAFR agonist activity when combined with UVB radiation in HaCaT cell culture, human explants, and murine models. 43 Additionally, immunosuppression has been found to be exacerbated by ethanol and TBI combination injury, where T-cell and dendrite cell counts were decreased by the ethanol exposure in rats. 44 Additionally, another study which confirms the increase in MVP levels due to ethanol exposure and intoxicated TBI, found that imipramine is also effective at decreasing MVP levels solely due to ethanol exposure, not just in the case of the combination injuries.…”

Section: Mvps and Itbimentioning

confidence: 99%

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Platelet‐activating factor and microvesicle particles as potential mediators for the toxicity associated with intoxicated thermal burn injury

et al. 2022

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Thermal burn injuries (TBIs) in patients who are alcohol-intoxicated result in greater morbidity and mortality. The systemic toxicity found in human patients, which includes both immediate systemic cytokine generation with multiple organ failure and a delayed systemic immunosuppression, has previously been replicated in mouse models combining ethanol and localized TBI. Though considerable insights have been provided with these models, the exact mechanisms for these pathologic effects are unclear. In this review, we highlight the roles of the lipid mediator platelet-activating factor (PAF) and subcellular microvesicle particle (MVP) release in response to intoxicated thermal burn injury (ITBI) as effectors in the pathology. Particularly, MVP is released from keratinocytes in response to PAF receptor (PAFR) activation due to excess PAF produced by ITBI. These subcellular particles carry and thus protect the metabolically labile PAF which enable binding of this potent lipid mediator to several key sites. We hypothesize that PAF carried by MVP can bind to PAFR within the gut, activating myosin light chain kinase (MLCK). The subsequent gut barrier dysfunction in response to MLCK activation then allows bacteria to invade the lymphatic system and, eventually, the bloodstream, resulting in sepsis and resultant dysregulated inflammation in multiple organs. PAF in MVP also activate the skin mast cell PAFR resulting in migration of this key effector cell to the lymph nodes to induce immunosuppression. This review thus provides a mechanism and potential therapeutic approaches for the increased toxicity and immunosuppressive outcomes of TBI in the presence of acute ethanol exposure.

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Ethanol consumption synergistically increases ultraviolet radiation induced skin damage and immune dysfunction

Brand

Stottlemyer

Paglia

et al. 2021

Journal of Dermatological Science

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Acute Ethanol Exposure Augments Low-Dose UVB-Mediated Systemic Immunosuppression via Enhanced Production of Platelet-Activating Factor Receptor Agonists

Cited by 8 publications

References 16 publications

Ultraviolet B Irradiation Alters the Level and miR Contents of Exosomes Released by Keratinocytes in Diabetic Condition

Ultraviolet B Irradiation Alters the Level and miR Contents of Exosomes Released by Keratinocytes in Diabetic Condition

Platelet‐activating factor and microvesicle particles as potential mediators for the toxicity associated with intoxicated thermal burn injury

Ethanol consumption synergistically increases ultraviolet radiation induced skin damage and immune dysfunction

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