Acute Exposure to Bisphenol A Causes Oxidative Stress Induction with Mitochondrial Origin in Saccharomyces cerevisiae Cells

Abstract

Bisphenol A (BPA) is a major component of the most commonly used plastic products, such as disposable plastics, Tetra Paks, cans, sport protective equipment, or medical devices. Due to the accumulation of excessive amounts of plastic waste and the subsequent release of BPA into the environment, BPA is classified as a pollutant that is undesirable in the environment. To date, the most interesting finding is the ability of BPA to act as an endocrine disrupting compound due to its binding to estrogen receptors (ERs), and adverse physiological effects on living organisms may result from this action. Since evidence of the potential pro-oxidizing effects of BPA has accumulated over the last years, herein, we focus on the detection of oxidative stress and its origin following BPA exposure using pulsed-field gel electrophoresis, flow cytometry, fluorescent microscopy, and Western blot analysis. Saccharomyces cerevisiae cells served as a model system, as these cells lack ERs allowing us to dissect the ER-dependent and -independent effects of BPA. Our data show that high concentrations of BPA affect cell survival and cause increased intracellular oxidation in yeast, which is primarily generated in the mitochondrion. However, an acute BPA exposure does not lead to significant oxidative damage to DNA or proteins.

Cornus mas L. Extract-Mediated Modulations of the Redox State Induce Cytotoxicity in Schizosaccharomyces pombe

“…The analysis of total reactive oxygen species (ROS) formation was performed according to Ďurovcová et al [35], with some optimization. The cells cultivated overnight were diluted to OD 600 = 1 and exposed to different treatments (0%, 0.5%, and 2% of ethanol; 0.5% and 2% of Cornus mas L. fruit ethanol extract) for 6 h. After the treatment, the samples were washed with PBS.…”

Acrylamide-Derived Ionome, Metabolic, and Cell Cycle Alterations Are Alleviated by Ascorbic Acid in the Fission Yeast

“…Generation of the total ROS was performed as previously described by [56] with slight modifications. Control and AA-treated cell cultures with and w/o AsA (10 mM) preincubation were adjusted to OD 600 = 1, washed with PBS, and incubated with 10 µM H 2 DCFDA (Sigma-Aldrich) at 30 • C in the dark for 1 h without shaking.…”