Acute Exposure to Glyphosate Herbicide Affects Oxidative Parameters in Piava (Leporinus obtusidens)

Abstract

In recent years, commercial glyphosate herbicide formulations have been widely used in agriculture to control aquatic weeds. These pesticides may result in disruption of ecological balance, causing damage to nontarget organisms including fish. Teleostean fish (Leporinus obtusidens) were exposed to commercial glyphosate herbicide formulation at 0 (control), 3, 6, 10 or 20 mg L(-1) for 96 h. The effects of herbicide on plasmatic metabolic parameters, thiobarbituric acid reactive substances (TBARS), catalase activity, protein carbonyl, and mucus layer parameters were studied. Plasmatic glucose and lactate levels increased but protein levels showed reduction after herbicide exposure. TBARS levels in brain showed a reduction at all tested concentrations. However, liver demonstrated increased TBARS levels at all tested concentrations, whereas in white muscle TBARS production did not change after exposure to herbicide. Fish exposed to all concentrations of glyphosate showed increase in liver catalase activity and protein carbonyl. Herbicide exposure increased protein and carbohydrate levels of the mucus layer at all tested concentrations. The present results showed that, in 96 h, glyphosate changed toxicological parameters analyzed in piava. Parameters measured in this study may be useful in environmental biomonitoring.

Atrazine-Mediated Oxidative Stress Responses and Lipid Peroxidation in the Tissues of Clarias gariepinus

“…AChE activity was measured as described by [16] and modified [17]. Lipid peroxidation was estimated by a thiobarbituric acid reactive substance (TBARS) assay [18]. The second portion of the homogenates was added to 8.1% sodium dodecyl sulfate (SDS) and 2.5M acetic acid (pH 3.34).…”

Toxicity of Sublethal Concentrations of Glyphosate and Paraquat Herbicide in the African Catfish Clarias gariepinus

“…A similar study was reported on Oreochromis niloticus by Ayoola (2008). Oxidative parameters were reported by Glusczak et al (2011) to be impacted in Piava (Leporinus obtusidens) on exposure to acute concentrations of glyphosate. Genotoxicity and cytotoxicity arising from glyphosate exposure have also been reported in the blood of fish by some other researchers (Cavalcante et al, 2008;Moreno et al, 2014).…”

Metabolic and Behavior Changes in Surubim Acutely Exposed to a Glyphosate-Based Herbicide

“…However, some investigators have shown that aquatic organisms, particularly fish, could be more sensitive to glyphosate compared with mammals (Lushchak et al 2009). Roundup formulation affected energy metabolism, hematological parameters, histological morphology, freeradical processes, and acetylcholinesterase activity in several fish species (Langiano & Martinez 2008;Lushchak et al 2009;Glusczak et al 2006Glusczak et al , 2007Glusczak et al , 2011Menezes et al 2011).…”