Acute phase proteins and antioxidant responses in queens with pyometra

Vilhena, Hugo; Figueiredo, Marta; Cerón, José J.; Pastor, Josep; Miranda, Sónia; Craveiro, Hélder; Pires, M.A.; Tecles, Fernando; Rubio, Camila Peres; Dąbrowski, Roman; Duarte, Sofia; Silvestre‐Ferreira, Ana C.; Tvarijonaviciute, Asta

doi:10.1016/j.theriogenology.2018.04.010

Cited by 25 publications

(42 citation statements)

References 68 publications

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“…Both TEAC assays evaluate the capacity of antioxidants in a sample, mainly albumin, ascorbic acid, urate, α‐tocopherol and bilirubin to reduce the radical 2,2′‐azino‐bis(3‐ethylbenzthiazoline‐6‐sulfonic acid) (ABTS) in the cation form (ABTS + ), however, diverge in the method of its synthesis; in the TEAC1 method, the ABTS is oxidized to ABTS + using hydrogen peroxide (H 2 O 2 ) in an acidic medium, while in the TEAC2 method, ABTS is oxidized through the use of the enzyme horse radish peroxidase . Overall the results obtained in the two assays are complementary …”

Section: Discussionsupporting

confidence: 77%

“…APP and biomarkers of oxidative status have proved to be clinically useful biomarkers in monitoring evolution and/or response to treatment of different feline diseases . However, the evolution of these analytes during the course of feline malignant mammary tumours has not been studied.…”

Section: Discussionmentioning

confidence: 99%

“…Serum GPox concentrations were determined by a commercial kit (Ransel, Randox Laboratories Limited, Crumlin, UK) previously validated for use in cats . Total serum thiol concentrations were determined by a method that uses aromatic disulfides as reagents, previously validated for use in cats . The FRAP assay uses the ferric‐tripyridyltriazine complex (Fe 3+ ‐TPTZ) as reagent, through a method previously validated for cats .…”

Section: Methodsmentioning

confidence: 99%

“…The FRAP assay uses the ferric‐tripyridyltriazine complex (Fe 3+ ‐TPTZ) as reagent, through a method previously validated for cats . The CUPRAC was determined by a method that uses bathocuproinedisulfonic acid disodium salt as chelating agent, previously validated for use in feline serum . The TEAC was determined by two different methodologies, namely TEAC using an acidic medium (TEAC1) and TEAC using the horse radish peroxidase (TEAC2), both methods previously validated for cats …”

Section: Methodsmentioning

confidence: 99%

“…35 Total serum thiol concentrations were determined by a method that uses aromatic disulfides as reagents, previously validated for use in cats. 36 The FRAP assay uses the ferric-tripyridyltriazine complex (Fe 3+ -TPTZ) as reagent, 32,37 through a method previously validated for cats. 36 The CUPRAC was determined by a method that uses bathocuproinedisulfonic acid disodium salt as chelating agent, 16,38 previously validated for use in feline serum.…”

Section: Antioxidants Analysismentioning

confidence: 99%

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Acute phase proteins and biomarkers of oxidative status in feline spontaneous malignant mammary tumours

Vilhena

Tvarijonaviciute

Cerón

et al. 2019

Vet Comparative Oncology

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Acute phase proteins (APP) and biomarkers of oxidative status change in human and canine mammary tumours, however, they have not been studied in feline mammary tumours. The aims of this study were to investigate the APP and antioxidant responses in feline malignant mammary tumours, to evaluate their relation with tumour features, and to assess their prognostic value. Serum amyloid A (SAA), haptoglobin (Hp), albumin, butyrylcholinesterase (BChE), insulin‐like growth factor1 (IGF1), paraoxonase1 (PON1), total serum thiols (Thiol), glutathione peroxidase (GPox) and total antioxidant capacity determined by different assays, including trolox equivalent antioxidant capacity assessed by two different methodologies (TEAC1/2), ferric reducing ability of plasma (FRAP), and cupric reducing antioxidant capacity (CUPRAC), were determined in serum of 50 queens with spontaneous mammary carcinomas and of 12 healthy female cats. At diagnosis, diseased queens presented significantly higher SAA and Hp, and lower albumin, BChE, GPox, TEAC1, TEAC2 and CUPRAC than controls. Different tumour features influenced concentrations of APP and antioxidants. Increases in serum Hp, and decreases in albumin, Thiol and FRAP were significantly associated with neoplastic vascular emboli, metastasis in regional lymph nodes and/or in distant organs. Distant metastasis development during the course of the disease was associated with increases in SAA and TEAC1. At diagnosis, decreased albumin was associated with a longer survival, and BChE <1.15 μmoL/mL.minute was associated with a shorter survival time on multivariate analysis. Feline malignant mammary tumours are associated with an APP response and oxidative stress, and different tumour features influence the inflammatory response and the oxidative damage. Furthermore, some of these analytes proved to have prognostic value.

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Section: Discussionsupporting

confidence: 77%

Section: Discussionmentioning

confidence: 99%