Acute Renal Failure and Toxic Nephropathy

Humes, H. David; Messana, Joseph M.

doi:10.1007/978-1-4613-0829-4_8

Cited by 2 publications

(2 citation statements)

References 125 publications

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“…The pathogenesis of ischemic renal tubular cell injury involves a complex interaction of various processes, including phospholipid alterations, depletion of high-energy phosphate stores, accumulation of oxygen free radicals, and calcium al-terations (1). Most evidence favors the idea that the critical events determining the course of cellular injury develop from damage to plasma and subcellular membranes (1).…”

Section: Introductionmentioning

confidence: 99%

“…Most evidence favors the idea that the critical events determining the course of cellular injury develop from damage to plasma and subcellular membranes (1). Phospholipids provide the major structural framework for cell membranes (2) and also participate in the regulation of membrane enzyme activity, permeability, and hormone activation (3)(4)(5)(6).…”

Section: Introductionmentioning

confidence: 99%

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Importance of adenosine triphosphate in phospholipase A2-induced rabbit renal proximal tubule cell injury.

Nguyen¹,

Cieslinski²,

Humes³

1988

J. Clin. Invest.

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The pathogenesis of ischemic renal tubular cell injury involves a complex interaction of different processes, including membrane phospholipid alterations and depletion of high-energy phosphate stores. To assess the role of membrane phospholipid changes due to activation of phospholipases in renal tubule cell injury, suspensions enriched in rabbit renal proximal tubule segments were incubated with exogenous phospholipase A2 (PLA2). Exogenous PLA2 did not produce any significant change in various metabolic parameters reflective of cell injury in control nonhypoxic preparations despite a significant decrease in phosphatidylethanolamine (PE) and moderate increases in lysophosphatidylcholine (LPC) and lysophosphatidylethanolamine (LPE). In contrast, exogenous PLA2 treatment of hypoxic tubules resulted in a severe degree of cell injury, as demonstrated by marked declines in tubule K+ and ATP contents and significant decreases in tubule uncoupled respiratory rates, and was associated with significant phospholipid alterations, including marked declines in phosphatidylcholine (PC) and PE and significant rises in LPC, LPE, and free fatty acids (FFA). The injurious metabolic effects of exogenous PLA2 on hypoxic tubules were reversed by addition of ATP-MgCI2 to the tubules. The protective effect of ATPMgCl2 was associated with increases in tubule PC and PE contents and declines in LPC, LPE, and FFA contents. These experiments thus indicate that an increase in exogenous PLA2 activity produces renal proximal tubule cell injury when cell ATP levels decline, at which point phospholipid resynthesis cannot keep pace with phospholipid degradation with resulting depletion of phospholipids and accumulation of lipid by-products. High-energy phosphate store depletion appears to be an important condition for exogenous PLA2 activity to induce renal tubule cell injury.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Importance of adenosine triphosphate in phospholipase A2-induced rabbit renal proximal tubule cell injury.

Nguyen¹,

Cieslinski²,

Humes³

1988

J. Clin. Invest.

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The Preclinical Study of Hyeolbuchugeo-tang (Xuefuzhuyu-tang) on Bone Healing in Rats with Rib Fracture

Huh

Oh²

2020

J Korean Med Rehabil

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Objectives The purpose of this study is to evaluate the healing effect of Hyeolbuchugeotang (HC) in rats with rib fracture. Methods Rats were randomly divided into 5 groups (naive, control, positive control, HC-L and HC-H). All groups except naive group were subjected to bone fracture of rib. Naive group received no treatment at all. Control group was fed with phosphate buffered saline. Positive control group was orally medicated with tramadol. Experimental group was orally medicated with HC extract (50 mg/kg for low concentration [HC-L], 100 mg/kg for high concentration [HC-H]). X-ray and micro-computed tomography (micro-CT) were conducted to assess the effect of HC. We analysed the level of 2) transforming growth factor-β1 (TGF-β1), Ki67, alkaline phosphatase (ALP), receptor activator of nuclear factor kappa-β, runt-related transcription factor 2 (Runx2) and tartrate resistant acid phosphatase (TRAP) on 7 and 14 days after fracture. ALP, alanine aminotransferase, aspartate aminotransferase, blood urea nitrogen, creatinine was measured for safety assessment. Results X-ray and micro-CT, showed HC enhance bone repair process. Callus formation was increased in experimental group at 7 days after fracture, but decreased at 14 days after fracture. 7 days after fracture, the level of TGF-β1 in experimental group was decreased. The level of Ki67, Runx2 in HC-H, TRAP in HC-L was increased. 14 days after fracture, the level of Ki67 in HC-L and HC-H was decreased. The level of ALP, Runx2, BUN in HC-L, TRAP in HC-L and HC-H was increased. Conclusions Taken together the results, HC promoted healing of bone fracture. In conclusion, HC has a potential to promote healing of bone fracture.

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Acute Renal Failure and Toxic Nephropathy

Cited by 2 publications

References 125 publications

Importance of adenosine triphosphate in phospholipase A2-induced rabbit renal proximal tubule cell injury.

Importance of adenosine triphosphate in phospholipase A2-induced rabbit renal proximal tubule cell injury.

The Preclinical Study of Hyeolbuchugeo-tang (Xuefuzhuyu-tang) on Bone Healing in Rats with Rib Fracture

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