Acute Skin Toxicity Characteristics of the Drug Based on Moxidectin

Abstract

The acute skin toxicity characteristics of the drug for veterinary use “Inspector Mini” were studied at mice and rats. The active ingredient of the drug is moxidectin which belongs to the group of macrocyclic lactones of the milbemycin class. The studies were carried out in the vivarium of VNIIP – FSC VIEV (Moscow, Russia) on 2 experimental and 1 control groups of white outbred male mice of 19–21 g, 10 animals in each group and male rats of 200–230 g, 6 individuals in each. The mass of animals was indicated during application of the drug. The drug was used once without dilution in the form of the provided solution with single-channel mechanical dispensers with a dosing volume of 10–100 μl for mice and 100–1000 μl for rats. The animals in the experimental group 1 were treated at a dose of 10 400 mg/kg (100 μl per 10 g of mouse body weight or 1000 μl per 100 g of rat body weight), animals in the experimental group 2 – at a dose of 5 200 mg/kg (50 μl per 10 g of mouse body weight or 500 μl per 100 g of rat body weight). The animals in control group were not treat with the drug. As a result of the study, it was found that the LD50 of the drug “Inspector Mini” applied to the skin of mice and rats was more than 10 400 mg/kg per animal weight. During clinical examination of laboratory animals from the experimental groups, no signs of intoxication were observed. During the experiment, there was no significant difference (p ≥ 0.05) in the animals weight from the experimental groups within all periods of weighing compared with the control group of analogues.