Acute stress disrupts intestinal homeostasis via GDNF‐RET

Lin, Lin; Feng, Bing-cheng; Zhou, Ran; Liu, Yi; Li, Lixiang; Wang, Kairuo; Yu, Yanbo; Liu, Chao; Long, Xin; Gu, Xiang; Li, Bing; Wang, Xiaojie; Yang, Xiaoyun; Cong, Yingzi; Zuo, Xiuli; Li, Yanqing

doi:10.1111/cpr.12889

Cited by 14 publications

(12 citation statements)

References 47 publications

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“…In contrast to our finding, Lin and colleagues 58 showed increased GDNF protein levels in colonic biopsies from patients with IBS-D.…”

Section: Ibs Symptoms Nevertheless the Present Study Shows Novel Resultscontrasting

confidence: 99%

“…These apparent contradictions may rely on several reasons such as number of study population and methods employed. Lin et al 58 studied a specific subgroup of patients with IBS-D, including both males and females. Lee and colleagues 59 also included both male and female patients in their study; however, they assessed mRNA levels only, which often show poor correlation with protein levels.…”

Section: Ibs Symptoms Nevertheless the Present Study Shows Novel Resultsmentioning

confidence: 99%

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Altered interaction between enteric glial cells and mast cells in the colon of women with irritable bowel syndrome

de-Faria

Casado‐Bedmar

Lindqvist

et al. 2021

Neurogastroenterology Motil

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Background: Enteric glial cells (EGC) and mast cells (MC) are intimately associated with gastrointestinal physiological functions. We aimed to investigate EGC-MC interaction in irritable bowel syndrome (IBS), a gut-brain disorder linked to increased intestinal permeability, and MC.Methods: Parallel approaches were used to quantify EGC markers in colonic biopsies from healthy controls (HC) and patients with IBS. Data were correlated with MC, vasoactive intestinal polypeptide (VIP) and VIP receptors (VPAC1/VPAC2) expressions, and bacterial translocation through biopsies mounted in Ussing chambers. In addition, we investigated the effects of EGC mediators on colonic permeability and the pharmacological-induced responses of EGC and MC cell lines.

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“…In contrast to our finding, Lin and colleagues 58 showed increased GDNF protein levels in colonic biopsies from patients with IBS-D.…”

Section: Ibs Symptoms Nevertheless the Present Study Shows Novel Resultscontrasting

confidence: 99%

Section: Ibs Symptoms Nevertheless the Present Study Shows Novel Resultsmentioning

confidence: 99%

Altered interaction between enteric glial cells and mast cells in the colon of women with irritable bowel syndrome

de-Faria

Casado‐Bedmar

Lindqvist

et al. 2021

Neurogastroenterology Motil

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“…A recent study showed that ELS triggers nerve growth factor (NGF) elevation, which directly targets ISC, stimulating their expansion and differentiation by trans-activating Wnt/β-catenin signaling, thus leading to intestinal ECs hyperplasia and 5-HT increase ( Wong et al, 2019 ). Furthermore, Lin et al (2020) showed that glial cell-derived neurotrophic factor (GDNF) rearranged during transfection is crucial for regulating ISC and ECs differentiation in wrap-restraint stress mice and in vitro . GDNF treatment amplifies Wnt signaling and increases 5-HT levels in colonic organoids in a dose-dependent manner.…”

Section: Factors Affecting Enterochromaffin Cells Synthesis Of 5-hydr...mentioning

confidence: 99%

Potential Roles of Enterochromaffin Cells in Early Life Stress-Induced Irritable Bowel Syndrome

Tao

Zhu

et al. 2022

Front. Cell. Neurosci.

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Irritable bowel syndrome (IBS) is one of the most common functional gastrointestinal disorders, also known as disorders of the gut–brain interaction; however, the pathophysiology of IBS remains unclear. Early life stress (ELS) is one of the most common risk factors for IBS development. However, the molecular mechanisms by which ELS induces IBS remain unclear. Enterochromaffin cells (ECs), as a prime source of peripheral serotonin (5-HT), play a pivotal role in intestinal motility, secretion, proinflammatory and anti-inflammatory effects, and visceral sensation. ECs can sense various stimuli and microbiota metabolites such as short-chain fatty acids (SCFAs) and secondary bile acids. ECs can sense the luminal environment and transmit signals to the brain via exogenous vagal and spinal nerve afferents. Increasing evidence suggests that an ECs-5-HT signaling imbalance plays a crucial role in the pathogenesis of ELS-induced IBS. A recent study using a maternal separation (MS) animal model mimicking ELS showed that MS induced expansion of intestinal stem cells and their differentiation toward secretory lineages, including ECs, leading to ECs hyperplasia, increased 5-HT production, and visceral hyperalgesia. This suggests that ELS-induced IBS may be associated with increased ECs-5-HT signaling. Furthermore, ECs are closely related to corticotropin-releasing hormone, mast cells, neuron growth factor, bile acids, and SCFAs, all of which contribute to the pathogenesis of IBS. Collectively, ECs may play a role in the pathogenesis of ELS-induced IBS. Therefore, this review summarizes the physiological function of ECs and focuses on their potential role in the pathogenesis of IBS based on clinical and pre-clinical evidence.

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“…WRS is an accepted method of creating an acute stress-induced IBS model [24]. In this study, we observed that treatment of Bi co signi cantly alleviated intestinal visceral hypersensitivity and reduced weight loss.…”

Section: Discussionmentioning

confidence: 67%

“…The IBS group and the IBS + Bi co group were induced by WRS procedures between 8 am and 10 am for 14 days. During forcing immobilization, they were placed in 50 mL tubes with a small hole for air and cotton ball were used to ll the extra space as described previously [24]. Subsequently, the IBS group was given an intragastric administration of PBS (10ml/kg) once a day and the IBS + Bi co group was given an intragastric administration of Bi co (0.78g/kg) Shanghai Sinepharm, Shanghai, China) once a day.…”

Section: Animalsmentioning

confidence: 99%

Bifico Relieves Irritable Bowel Syndrome By Regulating Gut Microbiota Dysbiosis and Inflammatory Cytokines in an Animal Model

Zhou

Zhang

Mao

et al. 2021

Preprint

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Gut microbiota dysbiosis, a core pathophysiology of irritable bowel syndrome (IBS), is closely related to immunological and metabolic functions. Gut microbiota-based therapeutics have been recently explored in several studies. Bifico is a probiotic cocktail widely used in gastrointestinal disorders which relate to the imbalance of gut microbiota. However, the efficacy and potential mechanisms of Bifico treatment in IBS remains incompletely understood. In this animal experiment, IBS mice received Bifico by intragastric administration. Subsequently, abdominal withdrawal reflex (AWR) scores showed a protective effect of Bifico in IBS mice. Then 16S rDNA, 1H nuclear magnetic resonance (1H-NMR) and western blot assays were performed to analyze alterations of gut microbiota, microbiome metabolites and inflammatory cytokines, respectively. Results suggested that while Bifico did not increase gut microbial diversity, it could change the composition of gut microbiota which were characterized by an increase of Proteobacteria phylum and Actinobacteria phylum, Muribaculum genera, Bifidobacterium genera and a decrease of Parabacteroides genera, Sutterella genera and Lactobacillus genera. Moreover, Bifico elevated the concentration of short-chain fatty acids (SCFAs) and reduced protein levels of interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α). From further Spearman's correlation analysis, Bifidobacterium genera were positively correlated with SCFAs including propionate, butyrate, valerate and negatively correlated with IL-6 and TNF-α. In conclusion, this study demonstrated that Bifico could alleviate symptoms of IBS mice through regulation of the gut microbiota, elevating production of SCFAs and reducing the colonic inflammatory response. Therefore, Bifico may have utility in clinical practice.

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Acute stress disrupts intestinal homeostasis via GDNF‐RET

Abstract: This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

Cited by 14 publications

References 47 publications

Altered interaction between enteric glial cells and mast cells in the colon of women with irritable bowel syndrome

Altered interaction between enteric glial cells and mast cells in the colon of women with irritable bowel syndrome

Potential Roles of Enterochromaffin Cells in Early Life Stress-Induced Irritable Bowel Syndrome

Bifico Relieves Irritable Bowel Syndrome By Regulating Gut Microbiota Dysbiosis and Inflammatory Cytokines in an Animal Model

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