Acute testicular toxicity of 1,3-dinitrobenzene and ethylene glycol monomethyl ether in the rat: Evaluation of biochemical effect markers and hormonal responses

Reader, S. C. J.; Shingles, C.; Stonard, Michael D.

doi:10.1016/0272-0590(91)90135-q

Cited by 55 publications

(15 citation statements)

References 20 publications

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“…Elevated level of FSH in EGEE-treated rats indicates that the normal restricting feedback from the gonad is absent, leading to an unrestricted pituitary FSH production, which is associated with subfertility and/or infertil-ity. Our data are in agreement with previous studies on insecticide methomyl (Mahgoub & El-Medany, 2001), ethylene glycol monomethyl ether, a member of glycol ethers family (Reader et al, 1991) and bisphenol A (Atanassova et al, 2000). The apparent reduction in EGEEmediated increase in FSH by kolaviron suggests its ability to preserve the intricate regulation between the testis and pituitary gland.…”

Section: Discussionsupporting

confidence: 92%

Chemoprotective effects of kolaviron on ethylene glycol monoethyl ether-induced pituitary-thyroid axis toxicity in male rats

Adedara

Farombi

2012

Andrologia

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Endocrine disrupting chemicals cause reproductive dysfunction by interacting with intricate regulation and cellular processes involve in spermatogenesis. This study investigated the probable mechanism of action of ethylene glycol monoethyl ether (EGEE) as an antiandrogenic compound as well as the effects of kolaviron upon co-administration with EGEE in rats. Adult male rats were exposed to EGEE (200 mg kg(-1) bw) separately or in combination with either kolaviron [100 (KV1) and 200 (KV2) mg kg(-1) bw] or vitamin E (50 mg kg(-1) bw) for 14 days. Western blot analysis revealed that the administration of EGEE adversely affected steroidogenesis in experimental rats by decreasing the expression of steroid acute regulatory (StAR) protein and androgen-binding protein (ABP). EGEE significantly decreased the activities of 3β-hydroxysteroid dehydrogenase (3β-HSD) and 17β-hydroxysteroid dehydrogenase (17β-HSD) but markedly increased sialic acid concentration in rat testes. EGEE-treated rats showed significant decreases in plasma levels of luteinising hormone (31%), testosterone (57.1%), prolactin (80.9%), triiodothyronine (65.3%) and thyroxine (41.4%), whereas follicle-stimulating hormone was significantly elevated by 76.9% compared to the control. However, co-administration of kolaviron or vitamin E significantly reversed the EGEE-induced steroidogenic dysfunction in rats. This study suggests that kolaviron may prove promising as a chemoprotective agent against endocrine pathology resulting from EGEE exposure.

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Section: Discussionsupporting

confidence: 92%

Chemoprotective effects of kolaviron on ethylene glycol monoethyl ether-induced pituitary-thyroid axis toxicity in male rats

Adedara

Farombi

2012

Andrologia

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“…Thus an infertile man with a high LDH-C4/sperm ratio would be suspected of having abnormally high rates of germ-cell degeneration during spermatogenesis. Studies in rats in which germ-cell degeneration was induced by administration of testicular toxicants has also shown that this leads to increased levels of LDH-C4 in peripheral blood for up to 14 days (Reader et al, 1991). Whilst this is an encouraging finding it is presumed that abnormally high levels of LDH-C4 are only detectable in semen or blood when active germ-cell degeneration is occurring (see also Eliasson & Virji, 1985).…”

Section: Results To Date On the Monitoring Of Spermatogenesis In Manmentioning

confidence: 99%

Monitoring of spermatogenesis in man‐measurement of Sertoli cell‐ or germ cell‐secreted proteins in semen or blood

Sharpe

1992

Int J of Andrology

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“…The ABP produced by SCs has also been proposed as a biomarker of the normal occurrence of spermatogenesis (Rehnberg et al, 1989). However, plasma concentration of ABP is not always directly associated with the concentrations detected in the testis and interstitial fluid (Reader et al, 1991;Rehnberg et al, 1989). Finally, the metabolic cooperation established between SC-germ cells has also been shown to serve as an indicator for the success of spermatogenesis.…”

Section: Discussionmentioning

confidence: 99%

Sertoli cell as a model in male reproductive toxicology: Advantages and disadvantages

Reis

Moreira

Sousa

et al. 2015

J of Applied Toxicology

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Pressure towards population aging in the demographic pyramid is not only due to sociological/personal choices but also due to subfertility or infertility. There are several chemicals and mixtures that impair male fertility. While experimental animal models are crucial to identify compounds that affect male fertility, it is essential to use reliable in vitro models to determine cellular targets and intracellular pathways that mediate chemical toxicity in the male reproductive system. In this review, we focused on the somatic Sertoli cell (SC) that, within the testis, is a major target for hormonal signaling and provides physical and nutritional support to developing germ cells. The different outcomes possible in each type of study: in vivo versus in vitro (either in primary or immortalized cell cultures) are analyzed. Herein, we intend to clarify the unique features that render SCs as excellent candidates for a robust in vitro model to study the deleterious effects of chemicals on male reproductive health. The sensitivity of SCs to toxicants/pharmaceuticals is discussed and, based on the literature reviewed we propose the in vitro study of SC physiology as a model to disclose deleterious effects of substances to male fertility.

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Acute testicular toxicity of 1,3-dinitrobenzene and ethylene glycol monomethyl ether in the rat: Evaluation of biochemical effect markers and hormonal responses

Cited by 55 publications

References 20 publications

Chemoprotective effects of kolaviron on ethylene glycol monoethyl ether-induced pituitary-thyroid axis toxicity in male rats

Chemoprotective effects of kolaviron on ethylene glycol monoethyl ether-induced pituitary-thyroid axis toxicity in male rats

Monitoring of spermatogenesis in man‐measurement of Sertoli cell‐ or germ cell‐secreted proteins in semen or blood

Sertoli cell as a model in male reproductive toxicology: Advantages and disadvantages

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