Acute toxicity and sub-chronic toxicity of steroidal saponins from Dioscorea zingiberensis C.H.Wright in rodents

Yuan, Qin; Wu, Xiaohua; Huang, Wen; Gong, Guohua; Li, Dan; He, Yang; Zhao, Yinlan

doi:10.1016/j.jep.2009.08.047

Cited by 104 publications

(60 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…To have an implication in the clinical setting, these concentrations should be attainable at the tissue level after administration of diosgenin. In the toxicity studies, oral administration of diosgenin was well tolerated [32, 33] and no evident toxicities were observed in mice given daily oral steroidal saponins (including 28 % diosgenin) extracted from rhizomes of Dioscorea zingiberensis at a dose of 127.5 mg/kg for 30 days [32]. In a pharmacokinetic study of a rat model, the peak plasma level of diosgenin was 23 ng/ml (55 nM) after oral administration of a dose of 10 mg/kg [34].…”

Section: Discussionmentioning

confidence: 99%

Diosgenin, an Activator of 1,25D3-MARRS Receptor/ERp57, Attenuates the Effects of TNF-α by Causing ADAM10-Dependent Ectodomain Shedding of TNF Receptor 1

Yang

Moon

Lee

et al. 2017

Cell Physiol Biochem

View full text Add to dashboard Cite

Background/Aims: We investigated how diosgenin, a steroidal sapogenin, has anti-tumor necrosis factor-α (TNF-α) effects in human aortic endothelial cells (HAECs). Methods: Tumor necrosis factor receptor 1 (TNFR1) was assessed by Western blot analysis. Intracellular Ca²⁺ was measured using Fluo-4 AM. Immunofluorescence staining was performed for a disintegrin and metalloprotease 10 (ADAM10). Results: Diosgenin (1 ∼ 100 nM) induced ectodomain shedding of TNFR1 within 30 min and attenuated TNF-α-induced intercellular adhesion molecule-1 (ICAM-1) expression. Upon treatment with diosgenin, extracellular Ca²⁺ entered into the cells via L-type calcium channels, whereas diosgenin-induced ectodomain shedding of TNFR1 was almost completely inhibited by BAPTA-AM (intracellular Ca²⁺ chelator), verapamil (L-type calcium channel antagonist) and the absence of extracellular Ca²⁺. Diosgenin caused translocation of ADAM10 to the cell surface, which was mediated by extracellular Ca²⁺ influx. Depletion of ADAM10 prevented diosgenin-induced ectodomain shedding of TNFR1 and abolished the inhibitory effect of diosgenin on TNF-α-induced ICAM-1 expression. Diosgenin did not induce extracellular Ca²⁺ influx and ectodomain shedding of TNFR1 in cells depleted of 1,25D₃-membrane associated rapid response steroid-binding receptor (1,25D₃-MARRS receptor/ERp57). Conclusion: Diosgenin elicits L-type calcium channel-mediated extracellular Ca²⁺ influx, and thereby induces ADAM10-mediated ectodomain shedding of TNFR1. This effect of diosgenin was exerted through 1,25D₃-MARRS receptor/ERp57.

show abstract

Section: Discussionmentioning

confidence: 99%

Diosgenin, an Activator of 1,25D3-MARRS Receptor/ERp57, Attenuates the Effects of TNF-α by Causing ADAM10-Dependent Ectodomain Shedding of TNF Receptor 1

Yang

Moon

Lee

et al. 2017

Cell Physiol Biochem

View full text Add to dashboard Cite

show abstract

“…Steroidal saponins obtained from Dioscorea zingiberensis are widely used for preventing cardiovascular diseases (Qin et al 2009). In addition to providing cardiovascular protection, steroidal saponin isolated from Ophiopogon japonicus plant also exhibited various other pharmacological activities, such as anticancer, immunomodulation, anti-oxidation, antiinflammation, cough relief, antimicrobial, and anti-diabetes .…”

Section: The Value Of Steroidal Saponinsmentioning

confidence: 99%

Recent advances in steroidal saponins biosynthesis and in vitro production

Upadhyay

Jeena

Shikha

et al. 2018

Planta

View full text Add to dashboard Cite

Main conclusion Steroidal saponins exhibited numerous pharmacological activities due to the modification of their backbone by different cytochrome P450s (P450) and UDP glycosyltransferases (UGTs). Plant-derived steroidal saponins are not sufficient for utilizing them for commercial purpose so in vitro production of saponin by tissue culture, root culture, embryo culture, etc, is necessary for its large-scale production.Saponin glycosides are the important class of plant secondary metabolites, which consists of either steroidal or terpenoidal backbone. Due to the existence of a wide range of medicinal properties, saponin glycosides are pharmacologically very important. This review is focused on important medicinal properties of steroidal saponin, its occurrence, and biosynthesis. In addition to this, some recently identified plants containing steroidal saponins in different parts were summarized. The high throughput transcriptome sequencing approach elaborates our understanding related to the secondary metabolic pathway and its regulation even in the absence of adequate genomic information of non-model plants. The aim of this review is to encapsulate the information related to applications of steroidal saponin and its biosynthetic enzymes specially P450s and UGTs that are involved at later stage modifications of saponin backbone. Lastly, we discussed the in vitro production of steroidal saponin as the plant-based production of saponin is time-consuming and yield a limited amount of saponins. A large amount of plant material has been used to increase the production of steroidal saponin by employing in vitro culture technique, which has received a lot of attention in past two decades and provides a way to conserve medicinal plants as well as to escape them for being endangered.

show abstract

“…Toxicology studies using relevant experimental models have established that even at an upper concentration of 3.5% (wt/wt), diosgenin was safe and failed to cause systemic toxicity, genotoxicity, or estrogenic activity (63). Qin et al (2009) reported that ethanol extracts of Dioscorea sp. containing 28.34% (wt/wt of lyophilized powder) did not cause any signs of acute toxicity in mice at an upper tested dose of 562.5 mg/kg/d, and did not significantly change toxicological parameters up to a dose of 255 mg/kg/d.…”

Section: Safetymentioning

confidence: 99%

Diosgenin, a Steroid Saponin Constituent of Yams and Fenugreek: Emerging Evidence for Applications in Medicine

Raju¹,

Rao²

2012

Bioactive Compounds in Phytomedicine

View full text Add to dashboard Cite

Acute toxicity and sub-chronic toxicity of steroidal saponins from Dioscorea zingiberensis C.H.Wright in rodents

Cited by 104 publications

References 26 publications

Diosgenin, an Activator of 1,25D3-MARRS Receptor/ERp57, Attenuates the Effects of TNF-α by Causing ADAM10-Dependent Ectodomain Shedding of TNF Receptor 1

Diosgenin, an Activator of 1,25D3-MARRS Receptor/ERp57, Attenuates the Effects of TNF-α by Causing ADAM10-Dependent Ectodomain Shedding of TNF Receptor 1

Recent advances in steroidal saponins biosynthesis and in vitro production

Diosgenin, a Steroid Saponin Constituent of Yams and Fenugreek: Emerging Evidence for Applications in Medicine

Contact Info

Product

Resources

About