Acute Toxicity of <em>Opuntia Ficus Indica</em> and <em>Pistacia Lentiscus</em> Seed Oils in Mice

Abstract

Opuntia ficus indica and Pistacia lentiscus L. seeds are used in traditional medicine. The objective of this study was to investigate the toxicity of the fixed oil of Opuntia ficus indica and Pistacia lentiscus L. seeds in mice through determination of LD 50 values, and also the physicochemical characteristics of the fixed oil of these oils. The acute toxicity of their fixed oil were also investigated in mice using the method of Kabba and Berhens. The fixed oil of Pistacia lentiscus and Opuntia ficus indica seeds were extracted and analyzed for its chemical and physical properties such as acid value, free fatty acid percentage (% FFA), iodine index, and saponification value as well as refractive index and density. LD 50 values obtained by single doses, orally and intraperitoneally administered in mice, were respectively 43 ± 0,8 ;[40.7-45.4 ] ml/kg body wt. p.o. and 2.72 ± 0,1 ;[2.52-2.92] ml/kg body wt. i.p. for Opuntia ficus indica ; and 37 ± 1 ; [34.4 -39.8 ] ml/kg body wt. p.o. and 2.52 ± 0,2 ;[2.22 -2.81 ] ml/kg body wt. i.p. for Pistacia lentiscus respectively. The yields of seed oil were respectively calculated as 20.25% and 10.41%. The acid and free fatty acid values indicated that the oil has a low acidity

Physicochemical Characteristics and Sub Chronic Oral Toxicity of <i>Pistacia lentiscus</i> L. Vegetable Oil in Rabbits

“…The value of the density measured for this oil was 0.9154 g/cm 3 . This value is consistent with the values reported by Boukeloua et al (2012), namely a density in the range of 0.918±0.02. The value is of the same order as those found in some vegetable oils; olive oil (0.910 to 0.916), palm oil (0.895 to 0.900), avocado oil (0.910 to 0.920) (Karleskind, 1992).…”

“…This value for our sample was 8.515 mg KOH g −1 . This value is closed to that reported by Boukeloua et al (2012) concerning this oil, which was 5.891 to 6.203. Charef et al (2008) have reported an acid values of 7.7±0.3 and 24±0.5 mg KOH g −1 , for lentisk oil respectively extracted from black and red berries.…”

“…The refractive index measured for the sample of this oil is 1.464. This value is closed to that reported by Boukeloua et al (2012) for the same oil (1.465±0.03) and those reported by Karleskind (1992), concerning olive oil, palm oil and avocado, which are respectively (1.468 to 1.470) (1.453 to 1.458) and (1.464 to 1.474).…”

Oxidative stress-related liver dysfunction by sodium arsenite: Alleviation byPistacia lentiscusoil

“…The dose of NaAsO 2 and the period of treatment were selected on the basis of previous studies (Pal & Chatterjee, 2004;Yousef et al, 2008), whereas Pistacia lentiscus oil dose was selected from previous studies on experimental animals (Boukeloua et al, 2012;Djerrou et al, 2010Djerrou et al, , 2011. All experimental procedures were carried out according to the National Institute of Health Guidelines for Animal Care and approved by the Ethics Committee of the University.…”

Effets of <i>Pistacia lentiscus</i> Virgin Fatty Oil on Lipidemic Profile and Carcass Characteristics in Hyperlipidemic <i>Oryctolagus cuniculus</i> Rabbits

“…Some pharmacological properties of this oil were recently investigated as cicatrizing activity (Djerrou et al, 2010), protection of mercury intoxication (Tounes et al, 2008), investigation of hepatoprotective effect against carbontetrachloride (Maameri et al, 2015). To ascertain the safety of this vegetable oil for its consumers, some studies were conducted as effects on glycemic index, liver and renal functions in rabbits (Djerrou et al, 2011), acute toxicity in mice (Boukeloua et al, 2012), irritancy potential and subacute dermal toxicity in rabbits (Djerrou et al, 2013), subchronic oral toxicity in rabbits (Maameri et al, 2016).…”