This study was carried out to determine acute toxicity of two heavy metals (zinc and copper) to fingerlings Percocypris pingi by static bioassays. The experimental fish were subjected to a range of concentrations of heavy metals. At different exposure periods (24 h, 48 h, 72 h and 96 h), the mortality rate were separately investigated. The median lethal concentration (LC 50 ) was determined with Probit analysis. The LC 50 values of zinc for the Percocypris pingi at 24 h, 48 h, 72 h and 96 h were 3.504 mg/L, 2.933 mg/L, 2.852 mg/L and 2.852 mg/L, respectively. But the LC 50 values of copper at 24 h, 48 h, 72 h and 96 h were 1.730 mg/L, 1.389 mg/L, 1.340 mg/L and 1.340 mg/L, respectively. The heavy metals were ranked in order of toxicity: copper >zinc. The safe concentration of zinc and copper were 0.2852 mg/L and 0.1340 mg/L, respectively. Physicochemical parameters variables e.g. dissolve oxygen, total hardness, and ammonia nitrogen of aquaria were monitored under different concentrations of zinc (0, 1.60, 2.40, 3.20, 4.00, 4.80, 5.60) mg/L and copper (0, 0.60, 1.00, 1.40, 1.80, 2.20, 2.60) mg/L.