Acute urinary retention and subsequent catheterization cause lipid peroxidation and oxidative DNA damage in the bladder: preventive effect of edaravone, a free‐radical scavenger

Abstract

30 min and then the bladder was allowed to drain with a catheter in place for 60 min as the studies continued. After killing the rats the function of the bladder was assessed, with carbachol and 100 m M KCl, and the levels of malondialdehyde (MDA, a marker of lipid peroxidation), 8-hydroxydeoxyguanosine (8-OHdG; a marker of oxidative DNA damage), heat-shock protein 70 (HSP 70) and its mRNA were measured. RESULTSAUR increased the intravesical pressure and decreased blood flow, and subsequent catheterization decreased the intravesical pressure and increased blood flow. Edaravone induced a decrease in blood flow in the bladder during the urinary retention and subsequent catheterization compared to the blood flow in the AUR group. Edaravone resulted in protection of the contractile responses to both carbachol and KCl in a dose-dependent manner. The MDA concentration, 8-OHdG content and expressions of HSP-70 and its mRNA in the AUR group were significantly larger than those of the control group. Edaravone markedly suppressed the accumulations of MDA and 8-OHdG in the bladder, and reduced the expressions of HSP 70 and its mRNA. CONCLUSIONThese results indicate that edaravone reduces the oxidative stress and prevents the bladder dysfunction caused by AUR and subsequent catheterization.

Beyond neurological disease: New targets for edaravone (Review)

“…Clinically, bladder dysfunction is sustained after acute urinary retention (AUR) (84). In turn, AUR and subsequent catheterization may enhance lipid peroxidation and oxidative DNA damage in the rat bladder.…”

Edaravone protects human peripheral blood lymphocytes from γ-irradiation-induced apoptosis and DNA damage

“…Previous research has shown that edaravone plays an important role as a direct and indirect free radical scavenger by neutralizing toxic ROS and reactive nitrogen species (RNS) (Roh et al 2011). Importantly, edaravone has been found to protect DNA from oxidative stress (Shimizu et al 2009). These mechanisms require the presence of edaravone at the time of radiation in cells and tissues.…”

Beyond free radical scavenging: Beneficial effects of edaravone (Radicut) in various diseases (Review)

“…Edaravone reduced the levels of HSP 70 (a marker of stress) and its mRNA in an I/R-induced rat testicular torsion model (31). Edaravone also markedly reduced the expression levels of HSP 70 and its mRNA and prevented bladder dysfunction caused by AUR and subsequent catheterization in rats (60).…”

“…Acute urinary retention (AUR) and subsequent catheterization caused lipid peroxidation and oxidative DNA damage in the rat bladder (60). Edaravone induced a decrease in blood flow in the bladder during urinary retention and subsequent catheterization (60).…”

“…Acute urinary retention (AUR) and subsequent catheterization caused lipid peroxidation and oxidative DNA damage in the rat bladder (60). Edaravone induced a decrease in blood flow in the bladder during urinary retention and subsequent catheterization (60). Edaravone protected the contractile responses to both carbachol and KCl and reduced the levels of MDA and 8-OHdG after AUR and subsequent catheterization-induced bladder dysfunction in rats (60).…”