Acyl-ACP thioesterases from castor (Ricinus communis L.): An enzymatic system appropriate for high rates of oil synthesis and accumulation

Sánchez‐García, Alicia; Moreno‐Pérez, Antonio J.; Muro‐Pastor, Alicia M.; Salas, Joaquı́n J.; Garcés, Rafael; Martı́nez-Force, Enrique

doi:10.1016/j.phytochem.2010.03.015

Cited by 53 publications

(60 citation statements)

References 60 publications

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“…Indeed, stronger expression of CsFatB in leaves was also reported in Ricinus communis (Sánchez-García, et al, 2010). Nevertheless, the expression patterns observed in this analysis suggests that Camelina sativa acyl-ACP thioesterases are important for oil deposition in the seed.…”

Section: Expression Profiles Of Camelina Sativa Acyl-acp Thioesterasesmentioning

confidence: 46%

“…WoLF PSORT was used to identify the signal peptide and the cleavage site where the mature protein begins, a program that predicts protein subcellular localization (Horton et al, 2007), with the information available for acyl-ACP thioesterases from Arabidopsis thaliana (Dörmann et al, 1995), Helianthus annuus (Martínez-Force et., al, 2000;Serrano-Vega et al, 2005) Gossypium hirsutum (Huynh et al, 2002), Diploknema butyracea (Jha et al, 2006), Madhuca longifolia (Ghosh and Sen, 2007), Ricinus communis (Sánchez-García et al, 2010) and Macadamia tetraphylla (Moreno-Pérez et al, 2011). Val-55 was the best candidate to represent the N-terminal amino acid of the mature CsFatA protein, corresponding to a signal peptide of 58 amino acid residues (Fig.…”

Section: Isolation and Sequence Analysis Of Acyl-acp Thioesterases Frmentioning

confidence: 99%

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Acyl-ACP thioesterases from Camelina sativa: Cloning, enzymatic characterization and implication in seed oil fatty acid composition

et al. 2014

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Acyl-acyl carrier protein (ACP) thioesterases are intraplastidial enzymes that terminate de novo fatty acid biosynthesis in the plastids of higher plants by hydrolyzing the thioester bond between ACP and the fatty acid synthesized. Free fatty acids are then esterified with coenzyme A prior to being incorporated into glycerolipids. Acyl-ACP thioesterases are classified into two families, FatA and FatB, which differ in their amino acid sequence and substrate specificity. Here, the FatA and FatB thioesterases from Camelina sativa seeds, a crop of interest in plant biotechnology, were cloned, sequenced and characterized. The mature proteins encoded by these genes were characterized biochemically after they were heterologously expressed in Escherichia coli and purified. Camelina sativa contained three different alleles of both the FatA and FatB genes. These genes were expressed most strongly in expanding tissues in which lipids are very actively synthesized, such as developing seed endosperm. The CsFatA enzyme displayed high catalytic efficiency on oleoyl-ACP and CsFatB acted efficiently on palmitoyl-ACP. The contribution of these two enzymes to the synthesis of Camelina sativa oil was discussed in the light of these results.

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Section: Expression Profiles Of Camelina Sativa Acyl-acp Thioesterasesmentioning

confidence: 46%

Section: Isolation and Sequence Analysis Of Acyl-acp Thioesterases Frmentioning

confidence: 99%

Acyl-ACP thioesterases from Camelina sativa: Cloning, enzymatic characterization and implication in seed oil fatty acid composition

et al. 2014

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“…Based on amino acid sequence and substrate specificities, Fat genes are classified into FatA and FatB. [12] In contrast to a reduction of C16:0 and C18:0 FAs, FatB from Chimonanthus praecox (CpFatB) and Lindera communis (LcFatB) increase accumulation of C14:0 and C16:1 FAs in transgenic lines of tobacco and L. communis, respectively. [13,14] Down-regulation of GmFatB through RNAi enabled soybeans to produce high levels of oleic acid (85%) and to reduce the palmitic acid content (<5%), which are regarded as desirable for agronomy and biofuel performance.…”

Section: Fa Biosynthesismentioning

confidence: 99%

Transport and transcriptional regulation of oil production in plants

Manan

Chen

She

et al. 2016

Critical Reviews in Biotechnology

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Triacylglycerol (TAG) serves as an energy reservoir and phospholipids as build blocks of biomembrane to support plant life. They also provide human with foods and nutrients. Multi-compartmentalized biosynthesis, trafficking or cross-membrane transport of lipid intermediates or precursors and their regulatory mechanisms are not fully understood. Recent progress has aided our understanding of how fatty acids (FAs) and phospholipids are transported between the chloroplast, the cytoplasm, and the endoplasmic reticulum (ER), and how the ins and outs of lipids take place in the peroxisome and other organelles for lipid metabolism and function. In addition, information regarding the transcriptional regulation network associated with FA and TAG biosynthesis has been further enriched. Recent breakthroughs made in lipid transport and transcriptional regulation has provided significant insights into our comprehensive understanding of plant lipid biology. This review attempts to highlight the recent progress made on lipid synthesis, transport, degradation, and their regulatory mechanisms. Metabolic engineering, based on these knowledge-powered technologies for production of edible oils or biofuels, is reviewed. The biotechnological application of metabolic enzymes, transcription factors and transporters, for oil production and composition improvement, are discussed in a broad context in order to provide a fresh scenario for researchers and to guide future research and applications.ARTICLE HISTORY

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“…Thioesterases play a pivotal role in fatty acid synthesis owing to their role in catalysing the terminal reaction of fatty acid biosynthesis, which regulates the fatty acid composition of storage lipids, especially in plant seeds (Brown et al 2010;Jing et al 2011). The expression of thioesterase genes displayed the highest levels in expanding tissues that are typically very active in lipid biosynthesis, such as developing seed endosperm and young expanding leaves (Oo and Stumpf 1979;Sánchez-García et al 2010).…”

Section: Discussionmentioning

confidence: 99%

“…Based on amino acid sequence alignments, these enzymes have been functionally characterised and classified into two general families, termed FatA and FatB (Jones et al 1995). All FatAs are orthologous in different species, with the highest activities towards oleoyl-ACP (18 : 1~9-ACP) (Hitr and Yadav 1992;Sánchez-García et al 2010). In contrast with the high level of conservation in the specificity of FatAs, FatBs primarily hydrolyse saturated acylACPs with chain lengths that contain between 8 and 18 carbons (Voelker and Davies 1994;Jones et al 1995;Jha et al 2010).…”

Section: Introductionmentioning

confidence: 99%

Molecular cloning and characterisation of an acyl carrier protein thioesterase gene (CocoFatB1) expressed in the endosperm of coconut (Cocos nucifera) and its heterologous expression in Nicotiana tabacum to engineer the accumulation of different fatty acids

Yang

Chen

Liang

et al. 2014

Functional Plant Biol.

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Abstract. Coconut (Cocos nucifera L.) contains large amounts of medium chain fatty acids, which mostly recognise acylacyl carrier protein (ACP) thioesterases that hydrolyse acyl-ACP into free fatty acids to terminate acyl chain elongation during fatty acid biosynthesis. A full-length cDNA of an acyl-ACP thioesterase, designated CocoFatB1, was isolated from cDNA libraries prepared from coconut endosperm during fruit development. The gene contained an open reading frame of 1254 bp, encoding a 417-amino acid protein. The amino acid sequence of the CocoFatB1 protein showed 100% and 95% sequence similarity to CnFatB1 and oil palm (Elaeis guineensis Jacq.) acyl-ACP thioesterases, respectively. Real-time fluorescent quantitative PCR analysis indicated that the CocoFatB1 transcript was most abundant in the endosperm from 8-month-old coconuts; the leaves and endosperm from 15-month-old coconuts had~80% and~10% of this level. The CocoFatB1 coding region was overexpressed in tobacco (Nicotiana tabacum L.) under the control of the seed-specific napin promoter following Agrobacterium tumefaciens-mediated transformation. CocoFatB1 transcript expression varied 20-fold between different transgenic plants, with 21 plants exhibiting detectable levels of CocoFatB1 expression. Analysis of the fatty acid composition of transgenic tobacco seeds showed that the levels of myristic acid (14 : 0), palmitic acid (16 : 0) and stearic acid (18 : 0) were increased by 25%, 34% and 17%, respectively, compared with untransformed plants. These results indicated that CocoFatB1 acts specifically on 14 : 0-ACP, 16 : 0-ACP and 18 : 0-ACP, and can increase medium chain saturated fatty acids. The gene may valuable for engineering fatty acid metabolism in crop improvement programmes.

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Acyl-ACP thioesterases from castor (Ricinus communis L.): An enzymatic system appropriate for high rates of oil synthesis and accumulation

Cited by 53 publications

References 60 publications

Acyl-ACP thioesterases from Camelina sativa: Cloning, enzymatic characterization and implication in seed oil fatty acid composition

Acyl-ACP thioesterases from Camelina sativa: Cloning, enzymatic characterization and implication in seed oil fatty acid composition

Transport and transcriptional regulation of oil production in plants

Molecular cloning and characterisation of an acyl carrier protein thioesterase gene (CocoFatB1) expressed in the endosperm of coconut (Cocos nucifera) and its heterologous expression in Nicotiana tabacum to engineer the accumulation of different fatty acids

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