Adalimumab ameliorates OVA-induced airway inflammation in mice: Role of CD4 + CD25 + FOXP3 + regulatory T-cells

Elsakkar, Mohamed G.; Sharaki, Olla A.; Abdallah, Dina; Mostafa, Dalia K.; Shekondali, Fadia T.

doi:10.1016/j.ejphar.2016.06.002

Cited by 11 publications

(8 citation statements)

References 54 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It was also found that CD4 + T cells were downregulated in the wild-type MLR, but the expression of CD4 + T cells in MLR with CNR2 knock-out did not change, suggesting a promoting role of the CNR2 on the proportion and activity of Treg cells. In their study of experimental autoimmune encephalomyelitis (EAE), Kong et al (47) found that the CNR2 selective agonist GP1a treatment of EAE mice inhibited the expression of RORγt, and IL-17 and Th17 differentiation, and enhanced the expression of FOXP3 and IL-10 and Treg differentiation (48,49), which suggested that CNR2 agonist activation may directly promote Treg differentiation, reduce Th17 differentiation, and affect the ratio and activity of Treg/Th17.…”

Section: Discussionmentioning

confidence: 99%

Selective activation of cannabinoid receptor 2 regulates Treg/Th17 balance to ameliorate neutrophilic asthma in mice

Wei¹,

Huang²,

Zheng³

et al. 2021

Ann Transl Med

View full text Add to dashboard Cite

Background: The cannabinoid receptor 2 (CNR2) plays a critical role in relieving asthma, with the mechanism still unclear. We aimed to investigate the mechanism of the CNR2 agonist (β-caryophyllene, β-Car) in regulating the balance of regulatory T cells (Treg) and T helper cell 17 (Th17) and thus its role in asthma. Methods:The study group of 50 pathogen-free female BALB/c mice were randomly divided at 6-8 weeks old into five groups of Control, Asthma, Asthma + β-Car (10 mg/kg), Asthma + β-Car + SR144528 (specific CNR2 antagonist, 3 mg/kg), and Asthma + β-Car + CMD178 (inhibitor of Treg cell, 10 mg/kg). ELISA was conducted to evaluate the main inflammatory cytokines [interleukin (IL)-6, IL-8, and tumor necrosis factor-α], and those secreted by Treg (transforming growth factor-β and IL-10), and Th17 (IL-17A and IL-22).Markers of Treg and Th17 cells were assessed by flow cytometry. In vitro, the CD4 + T cells were sorted and directed to differentiate to Treg and Th17 cells. The expression levels of CNR2, STAT5 and JNK1/2 were investigated by western blot and immunofluorescence assay.Results: β-Car relieved neutrophilic asthma severity in mice by elevating the marker genes' expression of Treg and inhibiting those of Th17, causing an increased proportion of Treg to Th17. β-Car also promoted the directed differentiation of CD4 + T cells into Treg, but not Th17. Activation of the CNR2 regulated the Treg/Th17 balance and relieved neutrophilic asthma possibly through promotion of phosphorylation of STAT5 and JNK1/2. Conclusions:The effect of the selective CNR2 agonist activating STAT5 and JNK1/2 signaling was to change the Treg/Th17 balance and reduce the inflammatory reaction, thus ameliorating neutrophilic asthma in a mouse model.

show abstract

Section: Discussionmentioning

confidence: 99%

Selective activation of cannabinoid receptor 2 regulates Treg/Th17 balance to ameliorate neutrophilic asthma in mice

Wei¹,

Huang²,

Zheng³

et al. 2021

Ann Transl Med

View full text Add to dashboard Cite

show abstract

“…The pro-inflammatory cytokine IL-6 is one of the key biomarkers in both ARDS patients and animal models that can predict the morbidity and mortality of ARDS patients [30, 31]. IFN-γ, a Th1 associated pro-inflammatory cytokine, is known to be essential in airway inflammation and inducing the influx of neutrophils [32, 33]. As a major inducer of CXCL10, excessive production of IFN-γ and CXCL10 contributed to injury progression in ARDS [34].…”

Section: Discussionmentioning

confidence: 99%

CXCL10/IP-10 Neutralization Can Ameliorate Lipopolysaccharide-Induced Acute Respiratory Distress Syndrome in Rats

Lang

Wang

et al. 2017

PLoS ONE

View full text Add to dashboard Cite

The role of C-X-C motif chemokine 10 (CXCL10), a pro-inflammatory factor, in the development of acute respiratory distress syndrome (ARDS) remains unclear. In this study, we explored the role of CXCL10 and the effect of CXCL10 neutralization in lipopolysaccharide (LPS)-induced ARDS in rats. The expression of CXCL10 and its receptor chemokine receptor 3(CXCR3) increased after LPS induction. Moreover, neutralization of CXCL10 ameliorated the severity of ARDS by reducing pulmonary edema, inhibiting the release of inflammatory mediators (IFN-γ, IL-6 and ICAM-1) and limiting inflammatory cells (neutrophils, macrophages, CD8+ T cells) influx into the lung, with a reduction in CXCR3 expression in neutrophils and macrophages. Therefore, CXCL10 could be a potential therapeutic target in LPS-induced ARDS.

show abstract

“…However, the IFN-γ levels were enhanced by the LED treatment in the BAL of the LPS-injected mice. IFN-γ is an important Th1 associated pro-inflammatory cytokine that inhibits the leukocyte influx into the lungs and it is mainly secreted by lymphocytes 35 , 36 . Moreover, IFN-γ secretion secretion is also important during a resolution of inflammation and it is responsible for a limitation of inflammation in a tissue injury 37 .…”

Section: Discussionmentioning

confidence: 99%

Beneficial effects of Red Light-Emitting Diode treatment in experimental model of acute lung injury induced by sepsis

Costa

Barioni

Ignácio

et al. 2017

Sci Rep

View full text Add to dashboard Cite

Sepsis is a severe disease with a high mortality index and it is responsible for the development of acute lung injury (ALI). We evaluated the effects of light-emitting diode (LED) on ALI induced by sepsis. Balb-c mice were injected with lipopolysaccharide or saline and then irradiated or not with red LED on their tracheas and lungs for 150 s, 2 and 6 h after LPS injections. The parameters were investigated 24 h after the LPS injections. Red LED treatment reduced neutrophil influx and the levels of interleukins 1β, 17 A and, tumor necrosis factor-α; in addition to enhanced levels of interferon γ in the bronchoalveolar fluid. Moreover, red LED treatment enhanced the RNAm levels of IL-10 and IFN-γ. It also partially reduced the elevated oxidative burst and enhanced apoptosis, but it did not alter the translocation of nuclear factor κB, the expression of toll-like receptor 4 (TLR4), as well as, oedema or mucus production in their lung tissues. Together, our data has shown the beneficial effects of short treatment with LED on ALI that are caused by gram negative bacterial infections. It is suggested that LED applications are an inexpensive and non-invasive additional treatment for sepsis.

show abstract

Adalimumab ameliorates OVA-induced airway inflammation in mice: Role of CD4 + CD25 + FOXP3 + regulatory T-cells

Cited by 11 publications

References 54 publications

Selective activation of cannabinoid receptor 2 regulates Treg/Th17 balance to ameliorate neutrophilic asthma in mice

Selective activation of cannabinoid receptor 2 regulates Treg/Th17 balance to ameliorate neutrophilic asthma in mice

CXCL10/IP-10 Neutralization Can Ameliorate Lipopolysaccharide-Induced Acute Respiratory Distress Syndrome in Rats

Beneficial effects of Red Light-Emitting Diode treatment in experimental model of acute lung injury induced by sepsis

Contact Info

Product

Resources

About