ADAMTS1 and ADAMTS5 metalloproteases produced by Sertoli cells: a potential diagnostic marker in azoospermia

Aydos, Oya Sena; Yükselten, Yunus; Özkavukçu, Sinan; Sunguroğlu, Asuman; Aydos, Kaan

doi:10.1080/19396368.2018.1467512

Cited by 17 publications

(9 citation statements)

References 41 publications

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“…ADAMTS5 protein expression in semen was significantly related with sperm production. [25] When compared with fertile control group, Aydos et al [26] have revealed the expression of ADAMTS5 was statistically significant lower in nonobstructive azoospermia. Researchers thought low ADAMTS expression might have an important role in the etiology of male infertility and might use as a predictive marker for azoospermic patients.…”

Section: Discussionmentioning

confidence: 99%

An in silico analysis of human sperm genes associated with asthenozoospermia and its implication in male infertility

Liu¹,

Cai

Liu³

2018

Medicine

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Section: Discussionmentioning

confidence: 99%

An in silico analysis of human sperm genes associated with asthenozoospermia and its implication in male infertility

Liu¹,

Cai

Liu³

2018

Medicine

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“…The extracted protein contents were separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis and then transferred onto polyvinylidene difluoride membranes. Afterward, the membrane was blocked with 5% StartingBlock Blocking Buffer (Thermo Scientific, Pittsburgh, PA, USA) for 24 h. When the blocking step was terminated, the membrane was incubated with the primary antibodies against anti-SF-1 (1:1000; Santa Cruz Biotechnology, Inc., Santa Cruz, California, USA), LHCGR (diluted at 1:500; Pro-teinTech, USA), FSHR (1:1000; Abcam, Cambridge, UK), and GAPDH (1:25,000; Abcam) at 4 C 21-23 for 24 h. The membrane was rinsed in phosphate-buffered saline (PBS)-Tween (005% Tween in PBS) three times (each time 5 min) and probed with horseradish peroxidase-conjugated antigoat or antirabbit secondary antibody (immunoglobulin G, diluted at 1:5000; Abcam) at room temperature for 1 h. 21 Finally, the protein bands were visualized using the enhanced chemiluminescence reagent kit (ECL-Plus; GE Healthcare), according to the manufacturer's instructions.…”

Section: Western Blot Analysismentioning

confidence: 99%

Evaluation of carvacrol on pituitary and sexual hormones and their receptors in the testicle of male diabetic rats

et al. 2020

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Diabetes mellitus (DM) is a complex metabolic disease and it is also closely associated with a reduction in fertility in male patients. The purpose of the present study was to investigate the antidiabetic effect of carvacrol (CRV), as a potent antioxidant, on the numbers of germ cells and Sertoli cells in testicular tissue, and the messenger RNA (mRNA) and protein expression of some genes involved in spermatogenesis, including luteinizing hormone/choriogonadotropin receptor ( LHCGR), follicle-stimulating hormone receptor ( FSHR), and steroidogenic factor 1 ( SF-1), as well as hormones such as luteinizing hormone (LH), follicle-stimulating hormone (FSH), testosterone (T), and insulin. Adult male Wistar rats ( n = 32) were randomly divided into four groups (eight animals per group), including healthy control that received 0.2% Tween 80, diabetic control group, the diabetic group treated orally with CRV (75 mg/kg), and CRV group that received orally CRV (75 mg/kg). The duration of the treatment period lasted 8 weeks. In the diabetic group, the numbers of Sertoli cells and germ cells were significantly decreased, while the treatment with CRV prevented the degree of the damage to the cells mentioned earlier. CRV administration elevated the concentrations of insulin, T, FSH, and LH. Moreover, treatment with CRV significantly enhanced the levels of the mRNA and protein expression of SF-1, LHCGR, and FSHR. According to the obtained results, CRV administration could prevent the deleterious effects of DM on testicular germ cells, and it increases the levels of hormones and some essential genes, such as SF-1, LHCGR, and FSHR, involved in the process of spermatogenesis.

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“…A disintegrin and metalloproteinase with thrombospondin motifs (ADAMTSs), which belong to the M12 metallopeptidase family, are responsible for the migration of differentiated germ cells through the seminiferous tubule wall by organizing the degradation and reformation of the ECM [ 31 ]. In fact, we recently demonstrated that ADAMTS1 and ADAMTS5 protein levels expressed in Sertoli cells were decreased approximately 2-fold in cases of NOA [ 32 ]. The effect of enzymatic digestion on the success of sperm retrieval in cases where these proteases are defective is a subject of further research.…”

Section: Processing and Selection Of Surgically Retrieved Sperm For Icsimentioning

confidence: 99%

Sperm Selection Procedures for Optimizing the Outcome of ICSI in Patients with NOA

Aydos

2021

JCM

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Retrieving spermatozoa from the testicles has been a great hope for patients with non-obstructive azoospermia (NOA), but relevant methods have not yet been developed to the level necessary to provide resolutions for all cases of NOA. Although performing testicular sperm extraction under microscopic magnification has increased sperm retrieval rates, in vitro selection and processing of quality sperm plays an essential role in the success of in vitro fertilization. Moreover, sperm cryopreservation is widely used in assisted reproductive technologies, whether for therapeutic purposes or for future fertility preservation. In recent years, there have been new developments using advanced technologies to freeze and preserve even very small numbers of sperm for which conventional techniques are inadequate. The present review provides an up-to-date summary of current strategies for maximizing sperm recovery from surgically obtained testicular samples and, as an extension, optimization of in vitro sperm processing techniques in the management of NOA.

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ADAMTS1 and ADAMTS5 metalloproteases produced by Sertoli cells: a potential diagnostic marker in azoospermia

Cited by 17 publications

References 41 publications

An in silico analysis of human sperm genes associated with asthenozoospermia and its implication in male infertility

An in silico analysis of human sperm genes associated with asthenozoospermia and its implication in male infertility

Evaluation of carvacrol on pituitary and sexual hormones and their receptors in the testicle of male diabetic rats

Sperm Selection Procedures for Optimizing the Outcome of ICSI in Patients with NOA

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