Adaptation of Fusarium oxysporum and Fusarium dimerum to the specific aquatic environment provided by the water systems of hospitals

Steinberg, Christian; Laurent, Julie; Edel-Hermann, Véronique; Barbezant, Marie; Sixt, Nathalie; Dalle, Frédéric; Aho, Serge; Bonnin, Alain; Hartemann, Philippe; Sautour, Marc

doi:10.1016/j.watres.2015.02.036

Cited by 20 publications

(17 citation statements)

References 35 publications

(55 reference statements)

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“…In addition, Fusarium can be found in aquatic habitats, including seawater, river water (20), and drinking water sources (21), and some populations seem to be particularly adapted to complex water distribution systems (22). Moreover, many Fusarium species are of clinical importance, causing, e.g., serious corneal infections (23) and invasive infections in immunocompromised patients (24).…”

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confidence: 99%

Genus-Specific Primers for Study of Fusarium Communities in Field Samples

Karlsson

Edel-Hermann

Gautheron

et al. 2016

Appl Environ Microbiol

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Fusarium is a large and diverse genus of fungi of great agricultural and economic importance, containing many plant pathogens and mycotoxin producers. To date, high-throughput sequencing of Fusarium communities has been limited by the lack of genusspecific primers targeting regions with high discriminatory power at the species level. In the present study, we evaluated two Fusarium-specific primer pairs targeting translation elongation factor 1 (TEF1). We also present the new primer pair Fa؉7/Ra؉6. Mock Fusarium communities reflecting phylogenetic diversity were used to evaluate the accuracy of the primers in reflecting the relative abundance of the species. TEF1 amplicons were subjected to 454 high-throughput sequencing to characterize Fusarium communities. Field samples from soil and wheat kernels were included to test the method on more-complex material. For kernel samples, a single PCR was sufficient, while for soil samples, nested PCR was necessary. The newly developed primer pairs Fa؉7/Ra؉6 and Fa/Ra accurately reflected Fusarium species composition in mock DNA communities. In field samples, 47 Fusarium operational taxonomic units were identified, with the highest Fusarium diversity in soil. The Fusarium community in soil was dominated by members of the Fusarium incarnatum-Fusarium equiseti species complex, contradicting findings in previous studies. The method was successfully applied to analyze Fusarium communities in soil and plant material and can facilitate further studies of Fusarium ecology.

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confidence: 99%

Genus-Specific Primers for Study of Fusarium Communities in Field Samples

Karlsson

Edel-Hermann

Gautheron

et al. 2016

Appl Environ Microbiol

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“…Fusaria in water may be aerosolized into the air when the water passes through taps and showers and then inhaled by immunocompromised patients (14). In two previous studies, we detected F. oxysporum in the water distribution systems of different French hospitals, with high concentrations of up to 10 5 propagules liter Ϫ1 in some units (19,20). Heterogeneous distributions according to building and variability over time were explained by episodic detachments of parts of colonies from biofilms in the pipes.…”

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confidence: 70%

“…With the exception of three previously analyzed isolates, all of the isolates displayed identical TEF1 sequences (19,20). The isolates were further characterized using microsatellite markers that were developed by Cheng-Hua Huang for populations of F. oxysporum f. sp.…”

Section: Methodsmentioning

confidence: 99%

“…Heterogeneous distributions according to building and variability over time were explained by episodic detachments of parts of colonies from biofilms in the pipes. Strains of F. oxysporum were shown to be particularly adapted to the specific conditions offered by the complex water systems of hospitals (20). Analysis of partial sequences of translation elongation factor 1␣ (TEF1) showed low genetic diversity within these strains.…”

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“…Analysis of partial sequences of translation elongation factor 1␣ (TEF1) showed low genetic diversity within these strains. Most of the isolates collected from water displayed the same sequence, even those from hospitals in two distant cities, suggesting the existence of a widespread clonal lineage in the water networks of hospitals (19,20). The question then arose as to the origin of this lineage.…”

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A Clonal Lineage of Fusarium oxysporum Circulates in the Tap Water of Different French Hospitals

Edel-Hermann

Sautour

Gautheron

et al. 2016

Appl Environ Microbiol

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Fusarium oxysporum is typically a soilborne fungus but can also be found in aquatic environments. In hospitals, water distribution systems may be reservoirs for the fungi responsible for nosocomial infections. F. oxysporum was previously detected in the water distribution systems of five French hospitals. Sixty-eight isolates from water representative of all hospital units that were previously sampled and characterized by translation elongation factor 1␣ sequence typing were subjected to microsatellite analysis and full-length ribosomal intergenic spacer (IGS) sequence typing. All but three isolates shared common microsatellite loci and a common two-locus sequence type (ST). This ST has an international geographical distribution in both the water networks of hospitals and among clinical isolates. The ST dominant in water was not detected among 300 isolates of F. oxysporum that originated from surrounding soils. Further characterization of 15 isolates by vegetative compatibility testing allowed us to conclude that a clonal lineage of F. oxysporum circulates in the tap water of the different hospitals. IMPORTANCEWe demonstrated that a clonal lineage of Fusarium oxysporum inhabits the water distribution systems of several French hospitals. This clonal lineage, which appears to be particularly adapted to water networks, represents a potential risk for human infection and raises questions about its worldwide distribution. N osocomial infections caused by members of the genus Fusarium have emerged over the past 2 decades (1, 2). Infections caused by Fusarium isolates may be superficial, such as keratitis and onychomycosis, but Fusarium spp. can also cause life-threatening disseminated infections in immunocompromised patients (3). The Fusarium species most often involved are the morphological species Fusarium solani and Fusarium oxysporum (4, 5). These morphological species are now recognized as species complexes due to their high level of phylogenetic diversity (6, 7).Members of the genus Fusarium have a diverse ecology that is linked to an impressive metabolic diversity together with a high genetic diversity. They are primarily known as saprophytes in the environment and are usually found in all types of soils (8-10), and they are frequently associated with plants as saprophytes, endophytes, or pathogens (9, 11). Fusaria may also be found in aquatic habitats, including natural water environments (12, 13) and drinking water distribution systems (14-17).Water distribution systems in hospitals were identified as potential reservoirs for species of Fusarium, where they are thought to be responsible for nosocomial infections (14, 18). Fusaria in water may be aerosolized into the air when the water passes through taps and showers and then inhaled by immunocompromised patients (14). In two previous studies, we detected F. oxysporum in the water distribution systems of different French hospitals, with high concentrations of up to 10 5 propagules liter Ϫ1 in some units (19,20). Heterogeneous distributions according to...

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Fusarium: Biodiversity, Ecological Significances, and Industrial Applications

Abdel-Azeem

Darwish

et al. 2019

Fungal Biology

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Adaptation of Fusarium oxysporum and Fusarium dimerum to the specific aquatic environment provided by the water systems of hospitals

Cited by 20 publications

References 35 publications

Genus-Specific Primers for Study of Fusarium Communities in Field Samples

Genus-Specific Primers for Study of Fusarium Communities in Field Samples

A Clonal Lineage of Fusarium oxysporum Circulates in the Tap Water of Different French Hospitals

Fusarium: Biodiversity, Ecological Significances, and Industrial Applications

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