Adaptation of human embryonic stem cells to feeder-free and matrix-free culture conditions directly on plastic surfaces

Bigdeli, Narmin; Andersson, Maria; Strehl, Raimund; Emanuelsson, Katarina; Kilmare, Eva; Hyllner, Johan; Lindahl, Anders

doi:10.1016/j.jbiotec.2007.08.045

Cited by 62 publications

(53 citation statements)

References 28 publications

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“…16 Cell culturing was performed at the Department of Clinical Chemistry and Transfusion Medicine, Institute of Biomedicine, University of Gothenburg. Note that the stem cells were used adhere to plastic surfaces and can be cultured in polystyrene dishes.…”

Section: B Human Embryological Stem Cell Culturementioning

confidence: 99%

“…In brief, cells were expanded in conditioned hES medium as described earlier 16 containing 80% KnockOut TM DMEM (Gibco-BRL/Invitrogen, pnr 10829018), 20% KnockOut serum replacement (Gibco-BRL/Invitrogen, 10828028), 2 mM L-glutamine (Gibco-BRL/ Invitrogen, 25030-024), 0.1 mM b-mercaptoethanol (Gibco-BRL/Invitrogen 31350-010), and 1% nonessential amino acids (Gibco-BRL/Invitrogen, 11140-035) on Primaria V R dishes (Falcon, surface modified polystyrene nonpyrogenic; Becton Dickinson, Franklin Lakes) and were incubated in a humidified atmosphere at 37 C and 5% CO 2 (Heraeus BBD6220). The SA167MFG-hESC and AS034.1MFG-hESC were passaged every 4-6 days, and the medium was changed every second day.…”

Section: Expansion Of Hescsmentioning

confidence: 99%

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Mg-corrosion, hydroxyapatite, and bone healing

et al. 2017

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The different capacities of magnesium in the metallic form (Mg-metal) and magnesium oxide (MgO) to stimulate bone healing are possible clues in the search for products that may promote bone healing. Since both Mg-metal and MgO can be assumed to release comparable amounts of Mg2+ ions during their reactions in the tissue where they have been implanted, it is of some importance to follow this process and analyze the resulting mineral formation in the tissue at the implantation site. Implants of MgO were inserted into rat tibia, and the bone healing was compared with sham-operated controls. Samples were taken after 1 week of healing and analyzed by histology, environmental scanning electron microscopy equipped with an energy dispersive x-ray spectroscopy analyzer, and time-of-flight secondary ion mass spectrometry (ToF-SIMS). Callus bone was seen in sham-operated controls after 1 week of healing. Implantation of MgO impaired the callus bone formation by replacing bone with apparently mineralized areas, lacking osteocytes and were denoted, amorphous bodies. Elemental analysis showed increased levels of Ca (7.1%), P (3.7%), and Mg (0.2%) in the bone marrow of MgO-treated animals versus sham-operated controls Ca (2.4%), P (2.3%), and Mg (0.1%). The Ca content of the cortical bone was also significantly increased (Ca, 29% increase) in MgO-treated animals compared to sham-operated controls. The Ca content of the cortical bone of sham-operated animals was also significantly (p < 0.05) higher than the corresponding value of untreated animals, which means that the surgical trauma induces an altered composition of the bone mineral. The Ca/P ratio was 1.26–1.68, which is compatible with that of mineralized bone with different contents of organic materials. Analysis of bone sections using ToF-SIMS showed the presence of hydroxyapatite (HA) and MgCO3 in the bone marrow and in cortical bone. Analysis using x-ray photoelectron spectroscopy of Mg, MgO, and MgCO3 after incubation with cell culture medium (DMEM), in vitro, showed binding of CaPO4 at the Mg and MgO samples. The Ca/P ratio was 0.8, indicating a higher P content than that expected for HA. Exposure of human embryonic stem cells to Mg species preincubated in DMEM resulted in HA production by the cells. Thus, two sources of CaPO4 in the bone marrow of MgO-treated bone were defined, catalytic formation on Mg-species and synthesis from activated stem-cells. The presented data suggest that bone healing near Mg implants is congruent with the fracture healing of bone, boosted by high HA levels in the bone marrow. In this context, the different capacities of Mg-metal and MgO to catalyse the formation of HA can be important clues to their different bone promoting effects.

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Section: B Human Embryological Stem Cell Culturementioning

confidence: 99%

Section: Expansion Of Hescsmentioning

confidence: 99%

Mg-corrosion, hydroxyapatite, and bone healing

et al. 2017

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“…Although they reported that the hES-MPs were more buffered against this diminishing capacity, it brings attention to the problem with serial passages, which are inexorably tied to the requirements of tissue engineering, and their resulting potential to undergo osteogenesis. The apparent discrepancy in relative Alk Phos activity was also found by Bigdeli and others (2010) when they compared the osteogenic capacity of human MSCs and a derived human ESC line (Bigdeli et al, 2008), which could be expanded on culture plastic without the support of feeder layers or other dish coatings such as Matrigel. Utilizing this cell line allowed the investigators to perform more direct comparison of the two cell types, since the typical differences between culture conditions were eliminated.…”

Section: Esc-derived Mscsmentioning

confidence: 63%

Osteogenesis from Pluripotent Stem Cells: Neural Crest or Mesodermal Origin?

Keller¹,

Nieden²

2011

Embryonic Stem Cells - Differentiation and Pluripotent Alternatives

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“…Because iPSCs are generated from one reprogrammed somatic cell, xeno-free methods to efficiently promote the clonal growth of single human ESCs are necessary. Bigdeli et al [85] reported that human ESC lines can be adapted to matrix-independent growth, even on plastic plates, by using a specified conditioned medium derived from human embryonic fibroblasts. This finding implies that it may be possible to develop a more effective defined culture medium that eliminates the need for a ESCs embryonic stem cells, iPSCs induced pluripotent stem cells, MEF-CM mouse embryonic fibroblast-derived conditioned medium, hES medium standard human ESC culture medium, mTeSR1 chemically defined xeno-free human ESC culture medium (Stemcell Technologies) substrate and thus achieves a feeder-free and xeno-free culture system for iPSCs.…”

Section: Discussionmentioning

confidence: 99%

Feeder Cell Sources and Feeder-Free Methods for Human iPS Cell Culture

Kamano

Wang

et al. 2015

Interface Oral Health Science 2014

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Induced pluripotent stem cells (iPSCs) hold great promise for regenerative medicine and disease modeling. The original methods to grow human iPSCs utilized methods developed for human embryonic cells (ESCs), in which mitotically inactivated mouse-derived fibroblasts are mainly used as a "feeder" cell layer to maintain the undifferentiated status of pluripotent stem cells. However, these methods still require further consideration to facilitate cell expansion and to maintain the undifferentiated state of human iPSCs and/or ESCs for a longer period of time. In addition, the use of animal-derived feeders should be avoided for eventual clinical application of iPSC therapies. Therefore, human-derived feeder culture systems or feeder-free culture systems are currently being developed to prevent exposure to animal pathogens. In this review, existing mouse and human feeder culture systems for human ESCs and iPSCs are first introduced, and then previously reported feeder-free culture methods using extracellular matrixassociated products or synthetic biomaterials are outlined to discuss an appropriate culture system for clinical application of iPSCs.

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Adaptation of human embryonic stem cells to feeder-free and matrix-free culture conditions directly on plastic surfaces

Cited by 62 publications

References 28 publications

Mg-corrosion, hydroxyapatite, and bone healing

Mg-corrosion, hydroxyapatite, and bone healing

Osteogenesis from Pluripotent Stem Cells: Neural Crest or Mesodermal Origin?

Feeder Cell Sources and Feeder-Free Methods for Human iPS Cell Culture

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