Adaptation of infectious bronchitis virus in primary cells of the chick embryo chorioallantoic membrane

Mohammed, M.H.; Hair-Bejo, M.; Zahid, A. H.; Alazawy, Amer; Ahad, E.A. Abdul; Hasoon, Mauida F.

doi:10.33899/ijvs.2013.82949

Cited by 3 publications

(1 citation statement)

References 10 publications

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“…Despite the fact that collective vaccination against infectious bronchitis has been practiced throughout the whole of the commercial chicken sector outbreaks of this illness have continued to occur up until the present day (14). Infectious bronchitis virus infections can also be identified by the identification of viral RNA by RT-PCR, as a result of which the diagnosis can be obtained quickly and reliably (15). The goal of this study is to detect any pathological changes in chicken embryos via allantoic sac inoculation and confirm with real-time RT-PCR.…”

Section: Introductionmentioning

confidence: 99%

The Effect of Infectious Bronchitis Virus In Embryonated Chicken Eggs

2023

JPTCP

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Specimens were collected from different farms in Iraq, then collected samples from (Baghdad, Babylon, Diyala, Wasit and Sallahulldin) were prepared by mortar and pestle and were inoculated into chicken embryos at age (9-11) days by allantoic sac inoculation. The death of embryos after 24 hr. post inoculation was ignored. There was no death of embryos on the first passage of the virus into chicken embryos and the allantoic fluid was harvested after 96 hours post inoculation. On second passage, one embryo was died and the allantoic fluid was harvested after 96 hr. post inoculation. On third passage, two embryos were died post inoculation and the allantoic fluid was harvested after 96 hr. The infected allantoic fluid was examined by RT-PCR to detect IBV. The result of RT-PCR was positive to detect IBV in infected harvested allantoic fluid.

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Section: Introductionmentioning

confidence: 99%

The Effect of Infectious Bronchitis Virus In Embryonated Chicken Eggs

2023

JPTCP

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Scanning electron microscopic exploration of intricate pollen morphology and antimicrobial potentials of gourd family

Alhomaidi

2024

Microscopy Res & Technique

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This study employs scanning electron microscopy (SEM) to delve into the intricate pollen morphology of Cucurbitaceae (Gourd Family) species, unraveling the nuanced details of their structural features. Concurrently, the research investigates the antimicrobial potentials encoded within these pollen grains, shedding light on their possible applications in the realm of natural antimicrobial agents. Cucurbitaceae plants showcases significant antibacterial and antifungal potentials, underscoring its potential as a source for novel antimicrobial compounds. This research endeavors to provide a comprehensive analysis of pollen morphometry in 12 Cucurbitaceous species, with the primary goal of identifying pollen morpho‐types to enrich the taxonomic understanding of the Cucurbitaceae. Following the Erdtman protocol, the study involved processing, measuring, and capturing pollen grains using SEM techniques. Qualitative data were analyzed to evaluate the variations in pollen types, size, and exine stratified sculptured layering. The pollen grains exhibit characteristics of being monads, ranging in size from medium to very large, with the prevailing shape being prolate‐spheroidal in six species. Notably, the maximum polar axis diameter was recorded for Cucurbita pepo (106.3 μm), while the minimum was observed in Zehneria anomala (32.6 μm). The grains of Cucurbitaceae exhibit diverse surface patterns, including reticulate columellae, reticulate, verrucate‐scabrate, verrucate‐gemmate, echinate baculate, reticulate‐perforate, and micro‐reticulate. This SEM investigation illuminates the intricacies of Cucurbitaceae pollen morphology while concurrently highlighting their noteworthy antimicrobial potentials.Highlights High‐resolution imaging reveals complex pollen morphology. Identification of unique antimicrobial structures on Cucurbitaceae pollen (gourd family). Exploring medicinal potential: Antimicrobial properties of cucurbitaceae species.

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