Adaptation of Saffold Virus 2 for High-Titer Growth in Mammalian Cells

Hertzler, Shannon; Liang, Zhiting; Tresó, Bálint; Lipton, Howard L.

doi:10.1128/jvi.00265-11

Cited by 15 publications

(19 citation statements)

References 28 publications

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“…The SafV are human viruses (4). They are yet to be linked to any particular human disease, although recent work suggests that they can persist in their hosts (5) and that SafV-2 has the potential to become neurotropic when inoculated intracerebrally into mice (6).…”

mentioning

confidence: 99%

Encephalomyocarditis Virus Leader Is Phosphorylated by CK2 and Syk as a Requirement for Subsequent Phosphorylation of Cellular Nucleoporins

Basta

Bacot‐Davis

Ciomperlik

et al. 2014

J Virol

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mentioning

confidence: 99%

Encephalomyocarditis Virus Leader Is Phosphorylated by CK2 and Syk as a Requirement for Subsequent Phosphorylation of Cellular Nucleoporins

Basta

Bacot‐Davis

Ciomperlik

et al. 2014

J Virol

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“…In general, whereas SAFV-3 has shown efficient growth and a clear cytopathic effect (CPE) in several cell lines, SAFV-2 strains are difficult to propagate and the detection of SAFV-2 has been mainly performed using molecular methods (5,9,12,(18)(19)(20). Chiu et al succeeded in isolating the SAFV-2 HTCV-UC6 strain only after the addition of monoclonal anti-cytokine antibodies (20).…”

Section: Communicated By Masayuki Saijomentioning

confidence: 99%

“…Although they analyzed viral tropism in vitro by using the HTCV-UC6 strain and 11 cell lines, they observed that viral replication was quite limited (20). Hertzler et al reported the successful adaptation of SAFV-2 to a limited number of cell lines, such as HeLa and U118MG, after repeated passaging in 14 human, non-human, and rodent cell lines (19). Galama et al and Blomqvist et al reported that the FIN2008 (or FIN08-13B) strain, which was the first SAFV-2 strain to grow well in green monkey kidney and HeLa cell lines, was needed to carry out seroepidemiological studies because most SAFV-2 isolates from their and other laboratories grew poorly in cell cultures, thus hampering the detection of neutralization (8,18).…”

Section: Communicated By Masayuki Saijomentioning

confidence: 99%

Isolation of Saffold Virus Type 2 from Children with Acute Respiratory Infections by Using the RD-18S-Niigata Cell Line

et al. 2015

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“…Hertzler et al found that high doses of SAFV-2 intracerebrally inoculated into FVB/n mice produced paralysis with neuropathological changes consistent with acute encephalomyelitis, particularly in the limbic system (25). Of special note was the presence of inflammation in the spinal cord white matter.…”

Section: The Pathogenicity Of Safv In Experimental Animalsmentioning

confidence: 99%

Saffold Virus, a Novel Human Cardiovirus with Unknown Pathogenicity

Himeda

Ohara

2012

J Virol

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Although cardioviruses have been thought to mainly infect rodents, a novel human cardiovirus, designated Saffold virus (SAFV), was identified in 2007. SAFV is grouped with Theiler-like rat virus and Theiler's murine encephalomyelitis virus (TMEV) in the species Theilovirus of the genus Cardiovirus of the family Picornaviridae. Eight genotypes of SAFV have now been identified. SAFV has been isolated from nasal and stool specimens from infants presenting with respiratory and gastrointestinal symptoms as well as from children with nonpolio acute flaccid paralysis; however, the relationship of SAFV to this symptomatology remains unclear. Of note, the virus has also been isolated from the cerebrospinal fluid specimens of patients with aseptic meningitis. This finding is of interest since TMEV is known to cause a multiple sclerosis-like syndrome in mice. The involvement of SAFV in various diseases (e.g., respiratory illness, gastrointestinal illness, neurological diseases, and type I diabetes) is presently under investigation. In order to clarify the pathogenicity of SAFV, additional epidemiological studies are required. Furthermore, identification of the SAFV cellular receptor will help establish an animal model for SAFV infection and help clarify the pathogenesis of SAFV-related diseases. In addition, investigation of the tissue-specific expression of the receptor may facilitate development of a novel picornavirus vector, which could be a useful tool in gene therapy for humans. The study of viral factors involved in viral pathogenicity using a reverse genetics technique will also be important.

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Adaptation of Saffold Virus 2 for High-Titer Growth in Mammalian Cells

Cited by 15 publications

References 28 publications

Encephalomyocarditis Virus Leader Is Phosphorylated by CK2 and Syk as a Requirement for Subsequent Phosphorylation of Cellular Nucleoporins

Encephalomyocarditis Virus Leader Is Phosphorylated by CK2 and Syk as a Requirement for Subsequent Phosphorylation of Cellular Nucleoporins

Isolation of Saffold Virus Type 2 from Children with Acute Respiratory Infections by Using the RD-18S-Niigata Cell Line

Saffold Virus, a Novel Human Cardiovirus with Unknown Pathogenicity

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