Adaptation of subpopulations of the Norway spruce needle endophyte Lophodermium piceae to the temperature regime

Müller, Michael M.; Hamberg, Leena; Морозова, Т. П.; Sizykh, Alexander; Sieber, Thomas N.

doi:10.1016/j.funbio.2019.09.008

Cited by 3 publications

(2 citation statements)

References 31 publications

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“…One challenge in these experiments is rearing of their larvae for application to the experimental trees. Relevant species include sawflies P. abietina and G. hercyniae and mites like N. haarlovi because they are native in northern Europe and their moderate to low occurrence could be a sign of sensitivity to L. piceae which is so common in needles of Norway spruce (Sieber 1988;Müller et al 2019). The needle-feeding nun moth L. monacha can cause large outbreaks in spruce forests (Bejer 1988;Liska and Srutka 1998) and could therefore be expected to be restricted by other factors than occurrence of L. piceae in the spruce needles.…”

Section: Discussionmentioning

confidence: 99%

Palatability of Norway spruce needles infected with Lophodermium piceae to larvae of two sawfly species

Müller,

Hamberg,

Varama

2023

Silva Fenn

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Unequivocal evidence on the antagonistic effects of endophytic fungi associated with woody plants against insect herbivores has been documented in only a few cases so far. Experimental evidence of the significance of needle endophytes to coniferous trees has remained scant because it is difficult to obtain trees with needles free of endophytes that could be used as comparable controls for trees infected with endophytes. Previously we reported a new methodology to get Norway spruce (Picea abies (L.) Karst.) saplings without needle endophytes and to inoculate them with a needle endophyte Lophodermium piceae (Fuckel) Höhn. Here we describe the first trial where spruce saplings with and without needle endophytes were provided as substrate for insect larvae. We transferred larvae of two sawfly species, Neodiprion sertifer Geoffroy and Gilpina pallida Klug, to the seedlings. Even though the main host of these sawfly species is not Norway spruce, but Scots pine (Pinus sylvestris L.), they are also known to occasionally feed on spruce. In this experiment the larvae did not develop to pupae with the provided spruce substrate but consumed measurable amounts of needles. No significant difference was found between the extent of needles consumed by either of the two sawfly species in inoculated and uninoculated saplings.

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Section: Discussionmentioning

confidence: 99%

Palatability of Norway spruce needles infected with Lophodermium piceae to larvae of two sawfly species

Müller,

Hamberg,

Varama

2023

Silva Fenn

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“…L. piceae has been found also in needles of Abies alba (Gourbière 1988;Minter 1994) and needles of Scottish P. sitchensis (Stewart et al, 2019). This fungus probably occurs in the whole distribution area of Norway spruce throughout Eurasia (Müller et al, 2019).…”

Section: Introductionmentioning

confidence: 96%

Eradication of endophytes from needles, their inoculation with Lophodermium piceae and persistence of this fungus in needles of Norway spruce

Müller

Hamberg

2021

Mycol Progress

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Lophodermium piceae is the most common endophyte of Norway spruce (Picea abies) needles, and it probably occurs in the total distribution area of Norway spruce. Its significance to the host tree is still largely unknown mainly because no methodology is known for generating endophyte-free saplings nor for inoculation of intact needles with this fungus. We report here how a daily heat treatment at 40 °C for 8 h eradicates endophytes of Norway spruce needles within a few days without causing visible harm to the plants. We compared various methods for inoculation of endophyte-free saplings. In order to obtain effective inocula, we investigated what factors activate dormant L. piceae infections in the needle tissue resulting in ascomata formation. Best inoculation success was achieved by suspending naturally shed, highly infected needles above the saplings. Infection frequencies of up to 70% of needles were achieved with repeated inoculations in the course of 3 years. Once established in needles, individual infections by L. piceae can persist for at least 5 years. Individual infections remain small in young needles, but at a needle age of 6 years, they start to grow and spread resulting in presence all over the needle from base to tip at a needle age of 7 years, but still without causing visible symptoms on the needles. The presented methodology for generating both endophyte-free and endophyte-infected saplings of Norway spruce provides a new approach for testing the significance of infections by L. piceae to insect herbivory and diseases of Norway spruce needles.

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The Large-Scale Investigation and Analysis of Lophodermium piceae in Subalpine Areas Based on Satellite Multi-Spectral Remote Sensing

et al. 2022

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Picea asperata, a common tree species in the subalpine areas of Li County, Sichuan Province, China, is susceptible to Lophodermium piceae. Remote sensing has the advantages of large-scale, fast information acquisition, and low cost, which can overcome the shortcomings of ground survey. Hence, we used Landsat 8 satellite multi-spectral images and forest resource distribution data to investigate and analyze this forest disease at a large scale. Firstly, we extracted the spatial distribution information of Picea asperata and chose a temporal sequence indicator to establish a regression model and obtained a significantly negative correlation between the damage degree of plants and the change rate of normalized difference vegetation index (NDVI). Accordingly, the investigation results of the disease have good consistency with the ground survey data in spatial distribution and damage degree. On this basis, a temporal regression analysis was performed by combining the remote sensing investigation results with climate variables, and canonical correspondence analysis (CCA) was utilized in the spatial comprehensive analysis of Lophodermium piceae with terrain, soil and forest stand factors. Conclusively, this study effectively coped with the difficulties in full investigation and analysis of Lophodermium piceae in ecologically fragile subalpine areas of Western Sichuan. It is of important reference value in the early warning and monitoring of this disease, and also provides objective and reliable information support for ecological restoration and management planning in the Wenchuan earthquake-stricken areas.

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Adaptation of subpopulations of the Norway spruce needle endophyte Lophodermium piceae to the temperature regime

Cited by 3 publications

References 31 publications

Palatability of Norway spruce needles infected with Lophodermium piceae to larvae of two sawfly species

Palatability of Norway spruce needles infected with Lophodermium piceae to larvae of two sawfly species

Eradication of endophytes from needles, their inoculation with Lophodermium piceae and persistence of this fungus in needles of Norway spruce

The Large-Scale Investigation and Analysis of Lophodermium piceae in Subalpine Areas Based on Satellite Multi-Spectral Remote Sensing

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